Pseudomonas phage vB_PaeP_130_113

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Krylovirinae; Phikmvvirus; Pseudomonas virus 130-113

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2R4P9G0|A0A2R4P9G0_9CAUD Uncharacterized protein OS=Pseudomonas phage vB_PaeP_130_113 OX=2161784 GN=C5022_000059 PE=4 SV=1
MM1 pKa = 7.32LCEE4 pKa = 4.98HH5 pKa = 7.13PLIDD9 pKa = 3.9PTTQAGLIRR18 pKa = 11.84AVAAYY23 pKa = 10.08QDD25 pKa = 5.01ALDD28 pKa = 4.47LCNALNQGDD37 pKa = 3.63

Molecular weight: 3.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2R4P9B8|A0A2R4P9B8_9CAUD Uncharacterized protein OS=Pseudomonas phage vB_PaeP_130_113 OX=2161784 GN=C5022_000020 PE=4 SV=1
MM1 pKa = 6.95TWLIIAVSPSGGCAFVWSRR20 pKa = 11.84KK21 pKa = 9.46RR22 pKa = 11.84PVRR25 pKa = 11.84PLRR28 pKa = 11.84FYY30 pKa = 11.01SRR32 pKa = 11.84KK33 pKa = 9.12AAKK36 pKa = 9.51RR37 pKa = 11.84WLRR40 pKa = 11.84KK41 pKa = 9.23HH42 pKa = 6.11RR43 pKa = 11.84RR44 pKa = 11.84AALLGSRR51 pKa = 11.84FLIVNWSKK59 pKa = 11.19RR60 pKa = 11.84II61 pKa = 3.62

Molecular weight: 7.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

62

0

62

14007

30

1337

225.9

25.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.087 ± 0.487

1.107 ± 0.195

5.897 ± 0.209

5.983 ± 0.232

3.248 ± 0.174

7.91 ± 0.278

2.056 ± 0.161

4.219 ± 0.178

4.498 ± 0.239

9.195 ± 0.259

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.963 ± 0.117

3.555 ± 0.226

4.655 ± 0.344

4.598 ± 0.311

6.939 ± 0.325

4.998 ± 0.227

5.433 ± 0.251

6.839 ± 0.326

1.699 ± 0.142

3.12 ± 0.23
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski