Terfezia boudieri ATCC MYA-4762

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; Pezizomycetes; Pezizales; Pezizaceae; Terfezia; Terfezia boudieri

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12026 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A3N4LIW4|A0A3N4LIW4_9PEZI Kinase-like protein OS=Terfezia boudieri ATCC MYA-4762 OX=1051890 GN=L211DRAFT_841522 PE=4 SV=1
MM1 pKa = 7.5TPPIPPQEE9 pKa = 4.49SQNSEE14 pKa = 3.82QPEE17 pKa = 4.18FQSLSLTDD25 pKa = 3.89SKK27 pKa = 11.45SSPKK31 pKa = 10.24LLPRR35 pKa = 11.84VLQDD39 pKa = 3.0TDD41 pKa = 3.95LVQPSGGAVSEE52 pKa = 4.21ISNSGEE58 pKa = 3.79SDD60 pKa = 3.59SEE62 pKa = 4.24SDD64 pKa = 3.92SEE66 pKa = 5.21SDD68 pKa = 3.61TEE70 pKa = 4.85SYY72 pKa = 10.36SADD75 pKa = 3.57YY76 pKa = 10.11EE77 pKa = 4.44SHH79 pKa = 6.0YY80 pKa = 10.74EE81 pKa = 3.91SYY83 pKa = 10.83YY84 pKa = 10.88EE85 pKa = 3.99SDD87 pKa = 3.43EE88 pKa = 4.34VSDD91 pKa = 4.7DD92 pKa = 3.7EE93 pKa = 4.98SSGVSDD99 pKa = 3.94YY100 pKa = 11.54EE101 pKa = 4.05SDD103 pKa = 3.93EE104 pKa = 4.53EE105 pKa = 4.5SDD107 pKa = 4.04CLSEE111 pKa = 4.04EE112 pKa = 4.06LGYY115 pKa = 9.64TSTEE119 pKa = 4.21SEE121 pKa = 4.57GEE123 pKa = 4.25SHH125 pKa = 6.92SDD127 pKa = 4.28SGDD130 pKa = 3.39TSDD133 pKa = 5.01DD134 pKa = 3.68TEE136 pKa = 4.54SDD138 pKa = 3.95YY139 pKa = 11.6EE140 pKa = 4.22SDD142 pKa = 4.0SEE144 pKa = 4.35YY145 pKa = 10.64GTLGSSEE152 pKa = 4.39DD153 pKa = 3.81SEE155 pKa = 5.51LEE157 pKa = 3.75QSGSEE162 pKa = 4.03EE163 pKa = 4.27SEE165 pKa = 4.16SGSEE169 pKa = 5.02DD170 pKa = 3.46GRR172 pKa = 11.84THH174 pKa = 5.67QQLAEE179 pKa = 3.99QTKK182 pKa = 10.57SIVTLEE188 pKa = 4.68CSPQLDD194 pKa = 3.34KK195 pKa = 11.47GDD197 pKa = 3.99RR198 pKa = 11.84EE199 pKa = 4.5GCRR202 pKa = 11.84RR203 pKa = 11.84ASGTFHH209 pKa = 7.24EE210 pKa = 4.89RR211 pKa = 11.84QQQ213 pKa = 3.28

Molecular weight:
23.19 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A3N4LER0|A0A3N4LER0_9PEZI Uncharacterized protein OS=Terfezia boudieri ATCC MYA-4762 OX=1051890 GN=L211DRAFT_852802 PE=4 SV=1
MM1 pKa = 7.59LLLRR5 pKa = 11.84LFRR8 pKa = 11.84MRR10 pKa = 11.84QMQLRR15 pKa = 11.84LHH17 pKa = 6.21KK18 pKa = 8.68TQLMLQHH25 pKa = 6.07KK26 pKa = 6.52TQLRR30 pKa = 11.84LQHH33 pKa = 5.62KK34 pKa = 6.32TQLRR38 pKa = 11.84LQHH41 pKa = 5.57KK42 pKa = 5.72TQHH45 pKa = 5.79RR46 pKa = 11.84MQHH49 pKa = 4.36KK50 pKa = 7.6TKK52 pKa = 10.81LRR54 pKa = 11.84MQHH57 pKa = 5.08KK58 pKa = 7.47TQLRR62 pKa = 11.84MQHH65 pKa = 4.94YY66 pKa = 9.5HH67 pKa = 6.28YY68 pKa = 10.21LTIFIFLLRR77 pKa = 11.84FLLKK81 pKa = 10.59HH82 pKa = 5.39SLTRR86 pKa = 11.84LLMQLSPTLRR96 pKa = 11.84LPRR99 pKa = 11.84LLRR102 pKa = 11.84TLRR105 pKa = 11.84LPRR108 pKa = 11.84LLRR111 pKa = 11.84TLRR114 pKa = 11.84LPRR117 pKa = 11.84LLRR120 pKa = 11.84LPRR123 pKa = 11.84LLRR126 pKa = 11.84PRR128 pKa = 11.84LLRR131 pKa = 11.84KK132 pKa = 9.65LRR134 pKa = 11.84TTKK137 pKa = 10.75

Molecular weight:
17.27 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12026

0

12026

4669809

49

5616

388.3

43.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.657 ± 0.021

1.308 ± 0.011

5.16 ± 0.015

6.773 ± 0.031

3.422 ± 0.014

7.083 ± 0.025

2.417 ± 0.012

5.117 ± 0.017

5.415 ± 0.026

8.899 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.197 ± 0.009

3.7 ± 0.014

6.3 ± 0.027

3.946 ± 0.02

6.18 ± 0.021

8.023 ± 0.025

6.096 ± 0.02

6.16 ± 0.018

1.385 ± 0.009

2.763 ± 0.014

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski