Osedax symbiont Rs1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Oceanospirillaceae; unclassified Oceanospirillaceae

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4383 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|S6GFF0|S6GFF0_9GAMM RDD domain-containing protein OS=Osedax symbiont Rs1 OX=1330036 GN=OFPII_21660 PE=4 SV=1
MM1 pKa = 7.53TSQHH5 pKa = 6.75HH6 pKa = 6.25IAQALDD12 pKa = 3.33FTTVRR17 pKa = 11.84KK18 pKa = 9.94IPAEE22 pKa = 3.71PTGAAKK28 pKa = 10.27IFQFHH33 pKa = 7.08CKK35 pKa = 9.0TNILHH40 pKa = 6.98SVFTLKK46 pKa = 10.71SRR48 pKa = 11.84IQVLVNCPFGFSLNNTEE65 pKa = 4.22VKK67 pKa = 10.39SMRR70 pKa = 11.84IGIGIVSLQTGIVVATGSAGVSRR93 pKa = 11.84VLHH96 pKa = 5.93LGEE99 pKa = 5.17TIFEE103 pKa = 5.18DD104 pKa = 4.91DD105 pKa = 5.85DD106 pKa = 5.08IDD108 pKa = 5.09LGADD112 pKa = 3.18STLEE116 pKa = 3.82VKK118 pKa = 10.45LYY120 pKa = 10.8NGEE123 pKa = 4.16TLTFKK128 pKa = 10.86QSLHH132 pKa = 5.35QVLLSKK138 pKa = 10.9YY139 pKa = 7.55VTSDD143 pKa = 3.02TSSHH147 pKa = 6.73FDD149 pKa = 3.49FLHH152 pKa = 5.96TDD154 pKa = 3.4AVASLTYY161 pKa = 10.61HH162 pKa = 7.13LDD164 pKa = 3.47PYY166 pKa = 11.59DD167 pKa = 3.57FLNPSRR173 pKa = 11.84EE174 pKa = 3.95HH175 pKa = 6.46HH176 pKa = 7.07SNFTFIVRR184 pKa = 11.84SAQEE188 pKa = 3.73IDD190 pKa = 3.22PTAGFDD196 pKa = 4.0PLSQSDD202 pKa = 4.55SIRR205 pKa = 11.84LEE207 pKa = 4.23PGLLSDD213 pKa = 4.42PAKK216 pKa = 10.73APPTTEE222 pKa = 3.87ASISGIFKK230 pKa = 10.28NPHH233 pKa = 6.01PASLHH238 pKa = 5.85IINNGFSNDD247 pKa = 3.32TSPVLRR253 pKa = 11.84GILSQKK259 pKa = 8.7LTAIEE264 pKa = 3.98EE265 pKa = 4.4LVVLRR270 pKa = 11.84NGIPIGKK277 pKa = 8.39ATLSNTTWNYY287 pKa = 9.87QDD289 pKa = 3.66SALEE293 pKa = 4.14TGHH296 pKa = 6.06QYY298 pKa = 10.83HH299 pKa = 6.03YY300 pKa = 9.25TVQVVSTDD308 pKa = 3.24GRR310 pKa = 11.84LSPLSAVYY318 pKa = 10.3SINIDD323 pKa = 3.76TIDD326 pKa = 3.81PAQIINITDD335 pKa = 3.22ITDD338 pKa = 4.7DD339 pKa = 3.52IAPKK343 pKa = 9.97IGSVADD349 pKa = 4.03GDD351 pKa = 4.53DD352 pKa = 4.41SNDD355 pKa = 3.6LAPKK359 pKa = 10.25IIGTLSSSLGTGEE372 pKa = 3.92VVNIYY377 pKa = 10.45RR378 pKa = 11.84DD379 pKa = 3.58GTLLGKK385 pKa = 9.09ATTVGLTWSFNDD397 pKa = 4.36SGLSDD402 pKa = 3.5ATSYY406 pKa = 11.58NYY408 pKa = 8.16TAQVIDD414 pKa = 3.61LAGNIGGLSNSYY426 pKa = 10.62NFNTDD431 pKa = 1.95ATAPMQTVIITDD443 pKa = 3.54ILDD446 pKa = 5.12DD447 pKa = 4.17ILPSTGNLAHH457 pKa = 7.62DD458 pKa = 5.0DD459 pKa = 4.07SSNDD463 pKa = 3.45TTPEE467 pKa = 3.9LLGTLSTALAANEE480 pKa = 3.99VLNILRR486 pKa = 11.84DD487 pKa = 3.74GVVIGQATVTGTNWRR502 pKa = 11.84YY503 pKa = 8.07TDD505 pKa = 4.18SGLLDD510 pKa = 4.13GNSYY514 pKa = 10.92DD515 pKa = 3.92YY516 pKa = 10.86SAQIIDD522 pKa = 3.78PASNLGPLSNLYY534 pKa = 9.24TINIDD539 pKa = 3.37TTAPTQTGNITDD551 pKa = 3.56IQDD554 pKa = 3.3NVAPLLGAILNNGFSNDD571 pKa = 4.19LSPEE575 pKa = 4.12LNGTLNATLASGEE588 pKa = 4.2VLNILRR594 pKa = 11.84DD595 pKa = 4.13GIIIGQATITGNNWSFNDD613 pKa = 3.48SALVDD618 pKa = 4.1GNSYY622 pKa = 11.13SYY624 pKa = 7.81TTQVSDD630 pKa = 3.26AAGNLGPLSTSHH642 pKa = 7.49TINIDD647 pKa = 3.33TSSPTQVISITDD659 pKa = 3.6INDD662 pKa = 4.4DD663 pKa = 3.46IAPNTGSVANGDD675 pKa = 3.98YY676 pKa = 11.28SNDD679 pKa = 3.5LAPEE683 pKa = 4.6IIGTLSNSLSAGEE696 pKa = 4.02VLNIYY701 pKa = 10.33RR702 pKa = 11.84DD703 pKa = 3.8GTLLGQATIAGLTWSFNDD721 pKa = 4.36SGLSDD726 pKa = 3.5ATSYY730 pKa = 11.58NYY732 pKa = 8.16TAQVIDD738 pKa = 3.75LAGNTGGLSNSYY750 pKa = 10.54NFNTNATAPTQMVVITDD767 pKa = 3.66ILDD770 pKa = 4.16DD771 pKa = 4.91LLPSTNNLAHH781 pKa = 6.87NDD783 pKa = 3.62SSNDD787 pKa = 3.47TTPEE791 pKa = 4.5LIGTLSTALAANEE804 pKa = 3.99VLNILRR810 pKa = 11.84DD811 pKa = 3.74GVVIGQAIVTGTNWRR826 pKa = 11.84YY827 pKa = 8.14TDD829 pKa = 4.46AGLLDD834 pKa = 4.42GNSYY838 pKa = 10.92DD839 pKa = 3.92YY840 pKa = 11.08SAQIIDD846 pKa = 3.54AASNLGTLSNLYY858 pKa = 9.21TINIDD863 pKa = 3.35TTAPTQIGNITDD875 pKa = 3.34IQDD878 pKa = 3.3NVAPLLGAILNNGFSNDD895 pKa = 4.19LSPEE899 pKa = 4.13LNGTLTAVLAAGEE912 pKa = 4.19VLNILRR918 pKa = 11.84DD919 pKa = 3.75GVIIGQATITGNNWSFNDD937 pKa = 3.47SALIDD942 pKa = 4.2GNSYY946 pKa = 11.15SYY948 pKa = 7.8TTQVSDD954 pKa = 3.02AAGNVGPLSTSHH966 pKa = 7.44TINIDD971 pKa = 3.33TSSPTQVISITDD983 pKa = 3.6INDD986 pKa = 4.4DD987 pKa = 3.46IAPNTGSVANGDD999 pKa = 3.98YY1000 pKa = 11.28SNDD1003 pKa = 3.5LAPEE1007 pKa = 4.59IIGTLSSSLSADD1019 pKa = 3.29EE1020 pKa = 4.66LVNIYY1025 pKa = 10.45RR1026 pKa = 11.84DD1027 pKa = 3.75GTLLGQATITGLTWSFNDD1045 pKa = 4.36SGLSDD1050 pKa = 3.5ATSYY1054 pKa = 11.58NYY1056 pKa = 8.16TAQVIDD1062 pKa = 3.75LAGNTGGLSNSYY1074 pKa = 10.52NFNTDD1079 pKa = 2.27TSTPTQTVIITDD1091 pKa = 3.59ILDD1094 pKa = 4.28DD1095 pKa = 5.07LLPSTNNLAHH1105 pKa = 6.87NDD1107 pKa = 3.62SSNDD1111 pKa = 3.47TTPEE1115 pKa = 4.5LIGTLSAALATNEE1128 pKa = 4.0VLNILRR1134 pKa = 11.84DD1135 pKa = 3.74GVVIGQAIVTGTNWRR1150 pKa = 11.84YY1151 pKa = 8.07TDD1153 pKa = 4.18SGLLDD1158 pKa = 4.13GNSYY1162 pKa = 11.0DD1163 pKa = 4.34YY1164 pKa = 11.16SAQINDD1170 pKa = 3.42AAGNLGTLSNLYY1182 pKa = 9.21TINIDD1187 pKa = 3.35TTAPTQIGNITDD1199 pKa = 3.34IQDD1202 pKa = 3.3NVAPLLGAILNNGFSNDD1219 pKa = 4.19LSPEE1223 pKa = 4.13LNGTLTAALAAGEE1236 pKa = 4.18VLNILRR1242 pKa = 11.84DD1243 pKa = 3.75GVIIGQATITGNNWNFDD1260 pKa = 3.52DD1261 pKa = 4.88SALVDD1266 pKa = 4.01GNSYY1270 pKa = 11.13SYY1272 pKa = 7.81TTQVSDD1278 pKa = 3.26AAGNLGPLSTSHH1290 pKa = 7.49TINIDD1295 pKa = 3.31TSNPTQIISITDD1307 pKa = 3.36INDD1310 pKa = 4.27DD1311 pKa = 3.34IAPYY1315 pKa = 8.85TGSVADD1321 pKa = 4.79GGDD1324 pKa = 3.96SNDD1327 pKa = 4.01LAPEE1331 pKa = 3.8ITGTLSSSLSTGEE1344 pKa = 3.78VVNIYY1349 pKa = 10.45RR1350 pKa = 11.84DD1351 pKa = 3.75GTLLGQATIAGLTWSFNDD1369 pKa = 4.36SGLSDD1374 pKa = 3.5ATSYY1378 pKa = 11.57NYY1380 pKa = 8.25TAQIIDD1386 pKa = 3.57LAGNLGGLSNSYY1398 pKa = 10.64NFNTDD1403 pKa = 2.53TTAPTQTVTITDD1415 pKa = 3.41IQDD1418 pKa = 3.47DD1419 pKa = 4.2VTPITGTISNGGISNDD1435 pKa = 4.48DD1436 pKa = 3.52APLLNGALSALLQVGEE1452 pKa = 4.41SVQILRR1458 pKa = 11.84NSTVIGTATVTGLSWSFNDD1477 pKa = 4.06SALVDD1482 pKa = 4.06GNSYY1486 pKa = 10.74SYY1488 pKa = 10.07QAQVIDD1494 pKa = 3.8SAGNLAALSANYY1506 pKa = 8.57TVTIDD1511 pKa = 3.63TSAPTQTVNIINITDD1526 pKa = 3.68DD1527 pKa = 3.31VSPFLGPVGHH1537 pKa = 6.89NGTTNDD1543 pKa = 3.88TAPLLSGTLSSLLNAGEE1560 pKa = 4.25LLDD1563 pKa = 4.68IIRR1566 pKa = 11.84DD1567 pKa = 3.74STVIGQAIVTGLTWSFNDD1585 pKa = 3.41SALSNGTSYY1594 pKa = 11.34NYY1596 pKa = 9.75RR1597 pKa = 11.84AQVRR1601 pKa = 11.84DD1602 pKa = 3.27NSGNLGPLANTFTLNIDD1619 pKa = 3.74TLAPAQTVTITDD1631 pKa = 3.16ILDD1634 pKa = 3.7NVLPSINNVAHH1645 pKa = 6.8NDD1647 pKa = 3.48SSNDD1651 pKa = 3.47TTPEE1655 pKa = 4.5LIGTLSTALATNEE1668 pKa = 3.87VLNILRR1674 pKa = 11.84DD1675 pKa = 3.74GVVIGQAIVTGTNWRR1690 pKa = 11.84YY1691 pKa = 8.14TDD1693 pKa = 4.46AGLLDD1698 pKa = 4.42GNSYY1702 pKa = 10.92DD1703 pKa = 3.92YY1704 pKa = 11.08SAQIIDD1710 pKa = 3.54AASNLGTLSNLYY1722 pKa = 9.21TINIDD1727 pKa = 3.37TTAPTQTGNITDD1739 pKa = 3.56IQDD1742 pKa = 3.3NVAPLLGAILNNSFSNDD1759 pKa = 3.25LSPEE1763 pKa = 4.13LNGTLTAALAAGEE1776 pKa = 4.18VLNILRR1782 pKa = 11.84DD1783 pKa = 3.75GVIIGQATITGNNWSFDD1800 pKa = 3.52DD1801 pKa = 4.13SALVDD1806 pKa = 4.01GNSYY1810 pKa = 11.13SYY1812 pKa = 7.81TTQVSDD1818 pKa = 3.26AAGNLGPLSTSHH1830 pKa = 7.49TINIDD1835 pKa = 3.31TSNPTQIISITDD1847 pKa = 3.36INDD1850 pKa = 4.27DD1851 pKa = 3.34IAPYY1855 pKa = 8.85TGSVADD1861 pKa = 4.79GGDD1864 pKa = 3.96SNDD1867 pKa = 4.01LAPEE1871 pKa = 3.8ITGTLSSSLSTGEE1884 pKa = 3.78VVNIYY1889 pKa = 10.45RR1890 pKa = 11.84DD1891 pKa = 3.75GTLLGQATIAGLTWSFNDD1909 pKa = 4.36SGLSDD1914 pKa = 3.5ATSYY1918 pKa = 11.57NYY1920 pKa = 8.25TAQIIDD1926 pKa = 3.57LAGNLGGLSNSYY1938 pKa = 10.64NFNTDD1943 pKa = 2.53TTAPTQTVTITDD1955 pKa = 3.32IQDD1958 pKa = 3.08NVAPITGTISNGGISNDD1975 pKa = 3.28NAPVLNGTLNAPLNTGEE1992 pKa = 4.24VVNILRR1998 pKa = 11.84FGVVIGQAIVTGTNWSYY2015 pKa = 12.06SDD2017 pKa = 4.28LGLVDD2022 pKa = 4.05GNNYY2026 pKa = 9.56IYY2028 pKa = 10.61NAQVADD2034 pKa = 3.71AAANLGVISASYY2046 pKa = 10.14AINIDD2051 pKa = 3.29TSSPVQTSNIVSIQDD2066 pKa = 3.4DD2067 pKa = 4.2LAPIISGIANGGITNDD2083 pKa = 3.72TSPLLNGTLSAILAAGEE2100 pKa = 3.96FLNVLRR2106 pKa = 11.84NGTVIGQAVVASTSWSFSDD2125 pKa = 4.62NALVDD2130 pKa = 4.18ANTYY2134 pKa = 9.8TYY2136 pKa = 7.38TTQVIDD2142 pKa = 3.32AAGNSGVLSNAYY2154 pKa = 10.26SITIDD2159 pKa = 3.27TTAPSQVVTITDD2171 pKa = 3.25IQDD2174 pKa = 3.41DD2175 pKa = 4.21VAPNTGSIASGGSSNDD2191 pKa = 3.74LAPQLIGILDD2201 pKa = 3.71SGLAGSEE2208 pKa = 4.27VINILRR2214 pKa = 11.84DD2215 pKa = 3.63GVIVGQASVSGLNWTFNDD2233 pKa = 4.12IGLADD2238 pKa = 3.83QSSFTYY2244 pKa = 9.47TAVIVDD2250 pKa = 3.96GAGNTGTVSNSYY2262 pKa = 10.6NFNTDD2267 pKa = 2.78AVATAAPTLGTVQDD2281 pKa = 3.98NVGAGQGSLNNGATTDD2297 pKa = 3.81DD2298 pKa = 3.94AKK2300 pKa = 10.43PTVNVLLPAGAVAGDD2315 pKa = 3.56TLALYY2320 pKa = 10.64NGATLIGTHH2329 pKa = 7.12TITAGEE2335 pKa = 4.26ITSGTASVEE2344 pKa = 3.85PSGNLTDD2351 pKa = 3.81ATHH2354 pKa = 6.48TLSTAIIDD2362 pKa = 3.76AAGNRR2367 pKa = 11.84SPNSASISLTVDD2379 pKa = 3.88TYY2381 pKa = 11.8NEE2383 pKa = 3.69AAAFLAAVTAGAARR2397 pKa = 11.84WGGHH2401 pKa = 5.61GGSHH2405 pKa = 5.61ITDD2408 pKa = 4.3PYY2410 pKa = 10.69VNVLALPAGSVVKK2423 pKa = 10.26LYY2425 pKa = 10.41VANNKK2430 pKa = 9.51VGEE2433 pKa = 4.19TSISVDD2439 pKa = 2.63GWSAHH2444 pKa = 4.87LTVSGSFAVSSGQYY2458 pKa = 8.23VHH2460 pKa = 6.95ATVTYY2465 pKa = 9.37NGVTRR2470 pKa = 11.84TVHH2473 pKa = 5.6TGHH2476 pKa = 7.15VFMNPGNTDD2485 pKa = 2.67ISFSSPLILDD2495 pKa = 3.96LGGNGIEE2502 pKa = 4.16TLNYY2506 pKa = 9.94SSGVLFDD2513 pKa = 4.45INADD2517 pKa = 3.33GKK2519 pKa = 8.82TEE2521 pKa = 3.78QTGWVGASDD2530 pKa = 3.93ALLVLDD2536 pKa = 5.26LNQDD2540 pKa = 3.52GLINDD2545 pKa = 3.48ASEE2548 pKa = 4.37LFGEE2552 pKa = 5.01HH2553 pKa = 6.15MLKK2556 pKa = 10.05QDD2558 pKa = 3.3GSKK2561 pKa = 11.0ASDD2564 pKa = 3.6GFDD2567 pKa = 4.01ALAQYY2572 pKa = 10.76DD2573 pKa = 4.24SNQDD2577 pKa = 3.35GVIDD2581 pKa = 4.11NQDD2584 pKa = 3.18QIYY2587 pKa = 10.04SQLLLWVDD2595 pKa = 3.97SNSDD2599 pKa = 3.87GISQADD2605 pKa = 3.6EE2606 pKa = 4.59LFTLEE2611 pKa = 4.57QRR2613 pKa = 11.84GITQLNLFSEE2623 pKa = 4.75RR2624 pKa = 11.84VNQNNNGNLTALEE2637 pKa = 4.86GYY2639 pKa = 10.57YY2640 pKa = 10.42LDD2642 pKa = 4.62NDD2644 pKa = 4.17GNRR2647 pKa = 11.84QLLSDD2652 pKa = 3.35VWFSYY2657 pKa = 10.69IEE2659 pKa = 4.06NNSAANQSDD2668 pKa = 3.92NLILDD2673 pKa = 3.88AADD2676 pKa = 3.25NVLAISEE2683 pKa = 5.24AIDD2686 pKa = 3.18KK2687 pKa = 10.33LQFSGIDD2694 pKa = 3.31ISPLQSFDD2702 pKa = 3.32YY2703 pKa = 10.55LHH2705 pKa = 6.93YY2706 pKa = 9.48YY2707 pKa = 9.81NKK2709 pKa = 10.24DD2710 pKa = 3.06ASISLDD2716 pKa = 3.54DD2717 pKa = 4.7LLEE2720 pKa = 4.41SSSNSEE2726 pKa = 3.83INADD2730 pKa = 3.12SAAAIFRR2737 pKa = 11.84YY2738 pKa = 10.3VFTHH2742 pKa = 5.99YY2743 pKa = 10.48SANSLLSSDD2752 pKa = 4.59DD2753 pKa = 4.25DD2754 pKa = 4.06NLVSQTSEE2762 pKa = 3.8TRR2764 pKa = 11.84PLYY2767 pKa = 10.1LQNLALFSTEE2777 pKa = 3.34QHH2779 pKa = 6.01QIIDD2783 pKa = 3.52ALLAEE2788 pKa = 4.78VASEE2792 pKa = 4.21SS2793 pKa = 3.62

Molecular weight:
291.51 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|S6HAH4|S6HAH4_9GAMM Valine-pyruvate aminotransferase OS=Osedax symbiont Rs1 OX=1330036 GN=OFPII_28940 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.49RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.91GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.31SGRR28 pKa = 11.84AVINRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.1GRR39 pKa = 11.84KK40 pKa = 8.79KK41 pKa = 10.63LSAA44 pKa = 3.95

Molecular weight:
5.08 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4383

0

4383

1210298

37

4414

276.1

30.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.003 ± 0.045

1.121 ± 0.016

5.26 ± 0.038

5.497 ± 0.034

4.082 ± 0.031

6.434 ± 0.046

2.163 ± 0.017

7.227 ± 0.032

5.626 ± 0.037

10.844 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.481 ± 0.025

4.294 ± 0.03

3.662 ± 0.023

4.943 ± 0.036

4.459 ± 0.029

7.153 ± 0.041

5.11 ± 0.036

6.527 ± 0.031

1.145 ± 0.013

2.964 ± 0.02

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski