Epulopiscium sp. SCG-B10WGA-EpuloA2

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Epulopiscium; unclassified Epulopiscium

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 477 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1V2MBA2|A0A1V2MBA2_9FIRM Uncharacterized protein OS=Epulopiscium sp. SCG-B10WGA-EpuloA2 OX=1712379 GN=AN642_00145 PE=4 SV=1
MM1 pKa = 7.23VFALDD6 pKa = 3.72RR7 pKa = 11.84CSQLYY12 pKa = 10.24ASNIVAVNSYY22 pKa = 11.38ASGFDD27 pKa = 3.13KK28 pKa = 11.15VLIGITCSITVISEE42 pKa = 4.3KK43 pKa = 10.57IGDD46 pKa = 4.02GYY48 pKa = 11.07SINNDD53 pKa = 2.94VAHH56 pKa = 6.91EE57 pKa = 4.28KK58 pKa = 10.14LPSMGYY64 pKa = 9.86IIEE67 pKa = 4.99DD68 pKa = 3.6LANTDD73 pKa = 4.22FSADD77 pKa = 3.47EE78 pKa = 4.67RR79 pKa = 11.84IEE81 pKa = 4.31EE82 pKa = 4.31YY83 pKa = 11.04LEE85 pKa = 4.2QDD87 pKa = 3.75DD88 pKa = 4.2STEE91 pKa = 4.09EE92 pKa = 3.74ITFEE96 pKa = 4.01EE97 pKa = 4.33QTEE100 pKa = 3.83QVYY103 pKa = 10.22ISKK106 pKa = 10.17EE107 pKa = 3.55AATFEE112 pKa = 4.35EE113 pKa = 4.55QTEE116 pKa = 3.84QVYY119 pKa = 10.24ISKK122 pKa = 10.18EE123 pKa = 3.63AVAFEE128 pKa = 4.24EE129 pKa = 4.22QTEE132 pKa = 3.92QVYY135 pKa = 10.24ISKK138 pKa = 10.18EE139 pKa = 3.63AVAFEE144 pKa = 4.24EE145 pKa = 4.22QTEE148 pKa = 3.92QVYY151 pKa = 10.21ISKK154 pKa = 10.2EE155 pKa = 3.92AACEE159 pKa = 3.67EE160 pKa = 4.19EE161 pKa = 4.86MYY163 pKa = 10.92QEE165 pKa = 4.35ILDD168 pKa = 4.35DD169 pKa = 4.16EE170 pKa = 4.67LFKK173 pKa = 10.78EE174 pKa = 4.1IQEE177 pKa = 4.48LIEE180 pKa = 5.0DD181 pKa = 4.31LFEE184 pKa = 4.4EE185 pKa = 4.78KK186 pKa = 10.19PIEE189 pKa = 4.14EE190 pKa = 4.16EE191 pKa = 4.81PILDD195 pKa = 3.79EE196 pKa = 5.62LIIEE200 pKa = 4.63IPDD203 pKa = 3.62LDD205 pKa = 3.66IVLEE209 pKa = 4.18

Molecular weight: 23.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1V2M9N7|A0A1V2M9N7_9FIRM DNA (cytosine-5-)-methyltransferase OS=Epulopiscium sp. SCG-B10WGA-EpuloA2 OX=1712379 GN=AN642_00265 PE=3 SV=1
MM1 pKa = 7.61AKK3 pKa = 10.41KK4 pKa = 10.4SMKK7 pKa = 10.03VKK9 pKa = 9.22QQRR12 pKa = 11.84PAKK15 pKa = 9.97FSTQAYY21 pKa = 8.08SRR23 pKa = 11.84CRR25 pKa = 11.84ICGRR29 pKa = 11.84PHH31 pKa = 7.05AYY33 pKa = 8.69IRR35 pKa = 11.84KK36 pKa = 8.79FGICRR41 pKa = 11.84VCFRR45 pKa = 11.84EE46 pKa = 4.01LAYY49 pKa = 9.83KK50 pKa = 10.19GQIPGVKK57 pKa = 9.25KK58 pKa = 10.9ASWW61 pKa = 3.03

Molecular weight: 7.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

477

0

477

138868

58

3624

291.1

32.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.05 ± 0.147

1.1 ± 0.052

5.705 ± 0.106

7.204 ± 0.121

4.475 ± 0.1

6.236 ± 0.127

1.448 ± 0.058

9.923 ± 0.126

7.809 ± 0.128

8.88 ± 0.134

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.528 ± 0.075

6.33 ± 0.118

3.08 ± 0.081

3.164 ± 0.082

2.997 ± 0.094

5.963 ± 0.088

5.997 ± 0.189

6.256 ± 0.091

0.801 ± 0.039

4.053 ± 0.094
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski