Acetobacter sp. DsW_54

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Acetobacteraceae; Acetobacter; unclassified Acetobacter

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2200 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A252B1G3|A0A252B1G3_9PROT Glutathione S-transferase OS=Acetobacter sp. DsW_54 OX=1670660 GN=HK20_08005 PE=4 SV=1
MM1 pKa = 7.7SIFNSLTTAVSGINAQSTAFTNLSNNIANSQTVGYY36 pKa = 9.79KK37 pKa = 10.26ADD39 pKa = 3.43TTAFQDD45 pKa = 4.32FVAGAGSTSGSFAQGVANSVAAVTVQHH72 pKa = 6.3VNSQGTASSSTDD84 pKa = 3.09GLAMSISGNGLFNVSKK100 pKa = 9.57KK101 pKa = 9.1TGAVTSAGADD111 pKa = 3.59FASEE115 pKa = 3.86QYY117 pKa = 8.4YY118 pKa = 9.05TRR120 pKa = 11.84NGEE123 pKa = 3.96FYY125 pKa = 10.28EE126 pKa = 5.04DD127 pKa = 3.38KK128 pKa = 10.88DD129 pKa = 4.01GYY131 pKa = 11.26LEE133 pKa = 4.17NTSGYY138 pKa = 9.93YY139 pKa = 10.19LDD141 pKa = 5.32GYY143 pKa = 9.91MVDD146 pKa = 3.67ATTGALDD153 pKa = 3.78TSSVTQINVANVTFRR168 pKa = 11.84PTQTTTITATGTLSSVTGASAVTTNTTVYY197 pKa = 10.83DD198 pKa = 3.58GTYY201 pKa = 10.46NQAKK205 pKa = 9.76PKK207 pKa = 10.82DD208 pKa = 3.68NTGDD212 pKa = 3.47VTLTWTQSSATEE224 pKa = 3.97WTVQASSSTTGMSVTPTTAYY244 pKa = 9.43TVDD247 pKa = 3.98FNTDD251 pKa = 2.86GSLKK255 pKa = 10.66SVTDD259 pKa = 3.45SSGNNVTSTLNGAAANLPISITYY282 pKa = 9.83PDD284 pKa = 4.65GNTQNVNVNIGTIGGTSGMSLSTSSTTPKK313 pKa = 10.38AVSDD317 pKa = 4.03SVTSGTYY324 pKa = 8.99QSASIEE330 pKa = 4.19SDD332 pKa = 3.31GSVMATFDD340 pKa = 3.95NGDD343 pKa = 3.37TQLIGKK349 pKa = 8.62VALSNFANVNGLLAQDD365 pKa = 3.95GQAYY369 pKa = 6.66TATAASGSAKK379 pKa = 9.82TGLVGEE385 pKa = 4.48NATGSLTVGYY395 pKa = 9.77VEE397 pKa = 6.08SSTTDD402 pKa = 3.29LTSDD406 pKa = 3.78LSALIVAQEE415 pKa = 4.3AYY417 pKa = 8.3TANTKK422 pKa = 9.9IVTTADD428 pKa = 3.12QLLQATIAMKK438 pKa = 10.36QQ439 pKa = 3.01

Molecular weight: 44.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A252B6A1|A0A252B6A1_9PROT Histidinol phosphate phosphatase OS=Acetobacter sp. DsW_54 OX=1670660 GN=HK20_01425 PE=4 SV=1
MM1 pKa = 7.49KK2 pKa = 10.22RR3 pKa = 11.84RR4 pKa = 11.84LARR7 pKa = 11.84FVEE10 pKa = 4.46LASKK14 pKa = 10.28GRR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84HH19 pKa = 4.8VAANDD24 pKa = 3.75TPGVRR29 pKa = 11.84IISWNLLRR37 pKa = 11.84RR38 pKa = 11.84TGATVHH44 pKa = 6.92DD45 pKa = 4.17VAALIEE51 pKa = 4.42AEE53 pKa = 4.18QPDD56 pKa = 4.19ILLMQEE62 pKa = 3.82ATVEE66 pKa = 4.02IDD68 pKa = 3.34VLPDD72 pKa = 3.41VIGGHH77 pKa = 5.71YY78 pKa = 10.16ARR80 pKa = 11.84SPLPGRR86 pKa = 11.84IHH88 pKa = 7.24GVACWSRR95 pKa = 11.84MPFARR100 pKa = 11.84PPRR103 pKa = 11.84ACTIPSGPIVKK114 pKa = 10.03RR115 pKa = 11.84HH116 pKa = 5.16AQIIDD121 pKa = 3.45YY122 pKa = 9.2PQFSLANVHH131 pKa = 6.64LSHH134 pKa = 6.68GQMLNRR140 pKa = 11.84RR141 pKa = 11.84QLRR144 pKa = 11.84RR145 pKa = 11.84IASLLSAPCAILGDD159 pKa = 4.17FNLVGPTLVPGFADD173 pKa = 3.87VGPKK177 pKa = 10.41APTHH181 pKa = 6.29RR182 pKa = 11.84MVDD185 pKa = 3.87LLPIRR190 pKa = 11.84LDD192 pKa = 3.3RR193 pKa = 11.84CLVEE197 pKa = 6.27GMVCSDD203 pKa = 3.5AQVLPVFASDD213 pKa = 3.5HH214 pKa = 6.24RR215 pKa = 11.84PIAVTLQPEE224 pKa = 4.19IGRR227 pKa = 11.84RR228 pKa = 11.84TLASAYY234 pKa = 10.07RR235 pKa = 3.68

Molecular weight: 25.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2200

0

2200

707855

52

1555

321.8

34.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.086 ± 0.067

1.087 ± 0.019

5.201 ± 0.037

5.109 ± 0.062

3.497 ± 0.034

8.367 ± 0.05

2.468 ± 0.023

4.706 ± 0.038

3.062 ± 0.035

10.462 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.661 ± 0.022

2.824 ± 0.034

5.546 ± 0.043

3.95 ± 0.035

6.597 ± 0.05

5.512 ± 0.047

5.826 ± 0.045

7.51 ± 0.047

1.325 ± 0.019

2.203 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski