Litoreibacter roseus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Litoreibacter

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4462 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6N6JDK3|A0A6N6JDK3_9RHOB Uncharacterized protein OS=Litoreibacter roseus OX=2601869 GN=KIN_12880 PE=4 SV=1
MM1 pKa = 7.23KK2 pKa = 10.13RR3 pKa = 11.84RR4 pKa = 11.84YY5 pKa = 8.69FAGAAALALVAGMAQADD22 pKa = 3.77GHH24 pKa = 5.73QPFAVGEE31 pKa = 4.26GDD33 pKa = 4.79FNWDD37 pKa = 3.11SYY39 pKa = 11.92NAFAEE44 pKa = 4.48KK45 pKa = 10.61YY46 pKa = 8.98DD47 pKa = 4.78LSGQTITITGPWTGLDD63 pKa = 3.6NDD65 pKa = 4.14NVTQVLSYY73 pKa = 10.83FEE75 pKa = 4.58SATGATVNYY84 pKa = 9.53SGSDD88 pKa = 3.17SFEE91 pKa = 3.75QDD93 pKa = 2.48IVISARR99 pKa = 11.84AGSAPNVAVFPQPGLAADD117 pKa = 4.72LASQGLLTPLPEE129 pKa = 4.44GTGDD133 pKa = 3.28WVQSNYY139 pKa = 10.71AAGQSWVDD147 pKa = 3.39LGTYY151 pKa = 9.37PGQDD155 pKa = 3.23GTEE158 pKa = 3.84DD159 pKa = 3.64LYY161 pKa = 11.69GFFYY165 pKa = 10.78KK166 pKa = 10.4VDD168 pKa = 3.56VKK170 pKa = 11.32SLVWYY175 pKa = 10.28SPEE178 pKa = 3.97AFDD181 pKa = 4.71EE182 pKa = 4.48AGYY185 pKa = 10.18DD186 pKa = 3.16IPEE189 pKa = 4.12TMEE192 pKa = 3.89EE193 pKa = 4.3LKK195 pKa = 11.02ALTDD199 pKa = 3.66QIVEE203 pKa = 4.16EE204 pKa = 5.8GGTPWCIGLGSGAATGWPATDD225 pKa = 3.12WVEE228 pKa = 5.29DD229 pKa = 3.51MMLRR233 pKa = 11.84TQSPEE238 pKa = 5.21DD239 pKa = 3.51YY240 pKa = 10.48DD241 pKa = 3.8AWVSNEE247 pKa = 4.14MKK249 pKa = 10.47FDD251 pKa = 4.02DD252 pKa = 4.88PKK254 pKa = 10.76VVAAIEE260 pKa = 4.34EE261 pKa = 4.21YY262 pKa = 10.86GYY264 pKa = 10.28FARR267 pKa = 11.84NNDD270 pKa = 3.71YY271 pKa = 11.43VDD273 pKa = 4.12GGASAVANTDD283 pKa = 3.85FRR285 pKa = 11.84DD286 pKa = 3.78SPTGLFDD293 pKa = 5.06IPPKK297 pKa = 10.57CYY299 pKa = 8.49MHH301 pKa = 6.78RR302 pKa = 11.84QASFIPTFFPEE313 pKa = 4.08DD314 pKa = 3.43AEE316 pKa = 4.3FDD318 pKa = 3.86FFYY321 pKa = 10.97FPAYY325 pKa = 9.54EE326 pKa = 4.4GKK328 pKa = 10.23DD329 pKa = 3.07LGKK332 pKa = 9.53PVLGAGTLWAITNPSDD348 pKa = 3.64GANALMEE355 pKa = 4.1YY356 pKa = 9.93LQTPIAHH363 pKa = 5.68EE364 pKa = 3.88TWMALSGFLTPHH376 pKa = 6.22SGVNTDD382 pKa = 4.32LYY384 pKa = 11.78ANATNKK390 pKa = 10.62ALGDD394 pKa = 3.67VLLNATTFRR403 pKa = 11.84FDD405 pKa = 4.8GSDD408 pKa = 3.54LMPGEE413 pKa = 4.38IGAGAFWTGMVDD425 pKa = 3.36YY426 pKa = 7.62TTGKK430 pKa = 10.29DD431 pKa = 3.13AAEE434 pKa = 4.1VAKK437 pKa = 10.59GIQDD441 pKa = 3.08RR442 pKa = 11.84WDD444 pKa = 3.91TIQQ447 pKa = 4.12

Molecular weight: 48.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6N6JEK4|A0A6N6JEK4_9RHOB Uncharacterized protein OS=Litoreibacter roseus OX=2601869 GN=KIN_18550 PE=4 SV=1
MM1 pKa = 7.1NRR3 pKa = 11.84IINMVLRR10 pKa = 11.84QLTRR14 pKa = 11.84QLVRR18 pKa = 11.84RR19 pKa = 11.84GVNSGLNYY27 pKa = 5.4MTKK30 pKa = 10.14RR31 pKa = 11.84GKK33 pKa = 10.39KK34 pKa = 9.07SAAKK38 pKa = 9.84ADD40 pKa = 3.77PSKK43 pKa = 10.52PVPNNAKK50 pKa = 8.93QNRR53 pKa = 11.84RR54 pKa = 11.84AMRR57 pKa = 11.84IMRR60 pKa = 11.84RR61 pKa = 11.84FGRR64 pKa = 11.84FF65 pKa = 2.78

Molecular weight: 7.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4462

0

4462

1347393

39

2131

302.0

32.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.556 ± 0.043

0.9 ± 0.011

6.403 ± 0.034

5.873 ± 0.036

3.928 ± 0.027

8.296 ± 0.036

2.033 ± 0.021

5.504 ± 0.026

3.451 ± 0.028

9.943 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.719 ± 0.017

2.778 ± 0.017

4.879 ± 0.025

3.291 ± 0.018

6.3 ± 0.033

5.542 ± 0.025

5.763 ± 0.023

7.212 ± 0.03

1.374 ± 0.016

2.253 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski