Rotavirus J

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Sedoreovirinae; Rotavirus

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L6BXM0|A0A1L6BXM0_9REOV ORF-X OS=Rotavirus J OX=1929964 GN=ORF-X PE=4 SV=1
MM1 pKa = 7.94LSMALFLLALLSITEE16 pKa = 3.76ARR18 pKa = 11.84INVGFVSPDD27 pKa = 3.07VCIVAEE33 pKa = 4.33RR34 pKa = 11.84QTSDD38 pKa = 2.9ADD40 pKa = 3.96KK41 pKa = 11.03INNLLDD47 pKa = 4.28FYY49 pKa = 10.6SFRR52 pKa = 11.84GFTYY56 pKa = 8.95EE57 pKa = 3.37ICQFNQDD64 pKa = 3.68ADD66 pKa = 4.39AIDD69 pKa = 5.27AISKK73 pKa = 9.08CSCLYY78 pKa = 11.03GNNTQIVAYY87 pKa = 9.64ADD89 pKa = 3.77TVKK92 pKa = 10.97SDD94 pKa = 3.32LATLLGNDD102 pKa = 3.6NDD104 pKa = 4.03CTVFTKK110 pKa = 9.08KK111 pKa = 8.33TKK113 pKa = 10.09KK114 pKa = 9.42WIPQYY119 pKa = 11.28DD120 pKa = 3.54RR121 pKa = 11.84DD122 pKa = 4.07RR123 pKa = 11.84EE124 pKa = 4.12YY125 pKa = 11.15FLYY128 pKa = 10.55AKK130 pKa = 9.92NVRR133 pKa = 11.84ICYY136 pKa = 9.78ISEE139 pKa = 3.97SLLGMGCDD147 pKa = 3.93STKK150 pKa = 10.39EE151 pKa = 4.02WTWQNITSTDD161 pKa = 3.55YY162 pKa = 11.4AIADD166 pKa = 3.91IPEE169 pKa = 3.97MTLDD173 pKa = 3.99GFWLFTTEE181 pKa = 3.6TAYY184 pKa = 11.25LCVRR188 pKa = 11.84KK189 pKa = 10.14DD190 pKa = 3.32SSQPKK195 pKa = 9.53PIYY198 pKa = 10.06FFNLIGNKK206 pKa = 9.61PPEE209 pKa = 4.26DD210 pKa = 3.38QIGQTRR216 pKa = 11.84SLATAWKK223 pKa = 8.86AVNSILHH230 pKa = 5.85FVYY233 pKa = 10.26KK234 pKa = 10.61VLDD237 pKa = 3.1IFYY240 pKa = 10.45RR241 pKa = 11.84EE242 pKa = 3.93RR243 pKa = 11.84LRR245 pKa = 4.8

Molecular weight: 28.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L6BXK9|A0A1L6BXK9_9REOV VP3 OS=Rotavirus J OX=1929964 GN=VP3 PE=4 SV=1
MM1 pKa = 7.94KK2 pKa = 8.91DD3 pKa = 3.17TQKK6 pKa = 11.44LMTIFQEE13 pKa = 4.65SSQHH17 pKa = 4.67QSQNLSILQKK27 pKa = 9.39ISRR30 pKa = 11.84MLRR33 pKa = 11.84LLRR36 pKa = 11.84RR37 pKa = 11.84NARR40 pKa = 11.84RR41 pKa = 11.84QRR43 pKa = 11.84YY44 pKa = 4.8YY45 pKa = 10.64QRR47 pKa = 11.84YY48 pKa = 8.37KK49 pKa = 9.85SRR51 pKa = 11.84LYY53 pKa = 10.47KK54 pKa = 10.44VEE56 pKa = 4.08TMGSNINSHH65 pKa = 6.06SSNFDD70 pKa = 2.75WKK72 pKa = 10.76KK73 pKa = 10.69

Molecular weight: 8.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13

0

13

6037

73

1168

464.4

53.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.41 ± 0.575

0.994 ± 0.26

5.996 ± 0.431

6.377 ± 0.425

4.174 ± 0.235

3.975 ± 0.387

1.706 ± 0.266

7.603 ± 0.413

6.907 ± 0.747

9.028 ± 0.69

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.7 ± 0.247

6.212 ± 0.473

3.843 ± 0.276

4.406 ± 0.517

5.665 ± 0.404

7.156 ± 0.6

6.245 ± 0.45

5.814 ± 0.425

0.994 ± 0.146

3.793 ± 0.383
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski