Rhodotorula sp. JG-1b

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Pucciniomycotina; Microbotryomycetes; Sporidiobolales; Sporidiobolaceae; Rhodotorula; unclassified Rhodotorula

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6678 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A109FG84|A0A109FG84_9BASI Uncharacterized protein OS=Rhodotorula sp. JG-1b OX=1305733 GN=RHOSPDRAFT_34523 PE=4 SV=1
MM1 pKa = 7.13LQSAPSGADD10 pKa = 2.95NDD12 pKa = 4.02YY13 pKa = 11.62DD14 pKa = 4.45DD15 pKa = 6.26LYY17 pKa = 11.54GDD19 pKa = 5.03LYY21 pKa = 10.46PAEE24 pKa = 4.47TEE26 pKa = 4.07ATPAASTSAAPSASATAPAAASPAGSTSTAAATGAPAATGYY67 pKa = 10.86GGDD70 pKa = 3.75AARR73 pKa = 11.84PAQLPQVPTGAAMPAAGGMVHH94 pKa = 7.6DD95 pKa = 5.07IGIPRR100 pKa = 11.84PQALQANALASKK112 pKa = 8.94TADD115 pKa = 3.35QLDD118 pKa = 3.82EE119 pKa = 4.28GG120 pKa = 4.45

Molecular weight: 11.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A109FAM0|A0A109FAM0_9BASI Uncharacterized protein (Fragment) OS=Rhodotorula sp. JG-1b OX=1305733 GN=RHOSPDRAFT_27953 PE=4 SV=1
MM1 pKa = 6.48THH3 pKa = 5.52EE4 pKa = 4.97AVWFSHH10 pKa = 4.79PRR12 pKa = 11.84KK13 pKa = 9.21YY14 pKa = 11.07GKK16 pKa = 10.25GSRR19 pKa = 11.84GCRR22 pKa = 11.84ICQHH26 pKa = 4.81QAGLIRR32 pKa = 11.84KK33 pKa = 8.7YY34 pKa = 10.83GLNICRR40 pKa = 11.84QCFRR44 pKa = 11.84EE45 pKa = 3.94RR46 pKa = 11.84SDD48 pKa = 5.08AIGFTKK54 pKa = 10.49NRR56 pKa = 3.76

Molecular weight: 6.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6678

0

6678

3457298

50

5368

517.7

56.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.128 ± 0.035

1.015 ± 0.011

5.688 ± 0.024

6.156 ± 0.031

3.305 ± 0.016

7.158 ± 0.027

2.287 ± 0.013

3.737 ± 0.021

4.139 ± 0.029

9.291 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.68 ± 0.009

2.688 ± 0.012

6.809 ± 0.041

3.781 ± 0.023

6.822 ± 0.024

8.621 ± 0.043

5.88 ± 0.02

6.226 ± 0.024

1.274 ± 0.01

2.313 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski