Chthoniobacter flavus Ellin428

Taxonomy: cellular organisms; Bacteria; PVC group; Verrucomicrobia; Spartobacteria; Chthoniobacterales; Chthoniobacteraceae; Chthoniobacter; Chthoniobacter flavus

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6705 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B4DBE6|B4DBE6_9BACT Uncharacterized protein OS=Chthoniobacter flavus Ellin428 OX=497964 GN=CfE428DRAFT_6237 PE=4 SV=1
MM1 pKa = 8.11DD2 pKa = 4.7EE3 pKa = 4.87SFLPTGSGTGPAGSTVDD20 pKa = 3.48TEE22 pKa = 4.36AFAGAFTHH30 pKa = 6.91VNGADD35 pKa = 3.75GASVSYY41 pKa = 10.07GLSVSASGVDD51 pKa = 3.09SGLIDD56 pKa = 3.96SLTGNHH62 pKa = 5.49VFLFLEE68 pKa = 4.38SGAVVARR75 pKa = 11.84EE76 pKa = 4.32GTTSATATTGPVEE89 pKa = 4.27FTLSVDD95 pKa = 3.11GSGNVTATLDD105 pKa = 3.59RR106 pKa = 11.84SVHH109 pKa = 5.59QGSADD114 pKa = 3.64SPVDD118 pKa = 3.73SNEE121 pKa = 3.82AVSLTSGLVTLTATITDD138 pKa = 3.54ADD140 pKa = 4.29GDD142 pKa = 4.14HH143 pKa = 6.14QAASIDD149 pKa = 3.96LGSHH153 pKa = 5.85VSIHH157 pKa = 7.5DD158 pKa = 4.61DD159 pKa = 3.91GPSITAVGTATALNVDD175 pKa = 4.16EE176 pKa = 5.67SFLPTGSGTGPTGSTVDD193 pKa = 3.49TEE195 pKa = 4.39AFAGAFTHH203 pKa = 6.91VNGADD208 pKa = 3.67GASISYY214 pKa = 9.46GLSVSASGVDD224 pKa = 3.09SGLIDD229 pKa = 3.96SLTGNHH235 pKa = 5.49VFLFLEE241 pKa = 4.38SGAVVARR248 pKa = 11.84EE249 pKa = 4.32GTTSATAATGPIEE262 pKa = 4.24FTLSVDD268 pKa = 3.12GSGNVTATLDD278 pKa = 3.66RR279 pKa = 11.84SVHH282 pKa = 5.15QSSADD287 pKa = 3.56SPVDD291 pKa = 3.68SNEE294 pKa = 3.82AVSLTSGLVTLTATITDD311 pKa = 3.54ADD313 pKa = 4.29GDD315 pKa = 4.14HH316 pKa = 6.14QAASIDD322 pKa = 3.96LGSHH326 pKa = 5.85VSIHH330 pKa = 7.5DD331 pKa = 4.61DD332 pKa = 3.91GPSITAVGTATALNVDD348 pKa = 4.16EE349 pKa = 5.67SFLPTGSGTGPAGSTVDD366 pKa = 3.48TEE368 pKa = 4.36AFAGAFTHH376 pKa = 7.28LNGADD381 pKa = 3.77GASVSYY387 pKa = 10.07GLSVSASGVDD397 pKa = 3.28SGLVDD402 pKa = 3.84SLTGNHH408 pKa = 5.48VFLFLEE414 pKa = 4.38SGAVVARR421 pKa = 11.84EE422 pKa = 4.14GTTSVTAATGPVEE435 pKa = 4.26FTLSVDD441 pKa = 3.11GSGNVTATLDD451 pKa = 3.66RR452 pKa = 11.84SVHH455 pKa = 5.15QSSADD460 pKa = 3.56SPVDD464 pKa = 3.68SNEE467 pKa = 3.82AVSLTSGLVALTATITDD484 pKa = 3.48ADD486 pKa = 4.29GDD488 pKa = 4.14HH489 pKa = 6.14QAASIDD495 pKa = 3.96LGSHH499 pKa = 5.85VSIHH503 pKa = 7.5DD504 pKa = 4.61DD505 pKa = 3.91GPSITAVGTATALNVDD521 pKa = 4.16EE522 pKa = 5.67SFLPTGSGTGPTGSTVDD539 pKa = 3.49TEE541 pKa = 4.39AFAGAFTHH549 pKa = 6.91VNGADD554 pKa = 3.67GASISYY560 pKa = 9.46GLSVSASGVDD570 pKa = 3.09SGLIDD575 pKa = 3.96SLTGNHH581 pKa = 5.49VFLFLEE587 pKa = 4.38SGAVVARR594 pKa = 11.84EE595 pKa = 4.06GATSVAAATGSIEE608 pKa = 4.07FTLSVDD614 pKa = 3.12GSGNVTATLDD624 pKa = 3.53RR625 pKa = 11.84SVHH628 pKa = 5.22QNSPDD633 pKa = 3.59SQVDD637 pKa = 3.27ANEE640 pKa = 4.2AVSLTSGLVTLTATITDD657 pKa = 3.39GDD659 pKa = 4.26GDD661 pKa = 4.05HH662 pKa = 6.33QAASIDD668 pKa = 3.96LGSHH672 pKa = 5.85VSIHH676 pKa = 7.48DD677 pKa = 4.57DD678 pKa = 3.77GPTITAIQNLIMPNIDD694 pKa = 3.09NTVDD698 pKa = 2.84HH699 pKa = 6.32GTWVPVFGADD709 pKa = 3.82GPSATSAVSVLMGTPPAGTTYY730 pKa = 10.58TVTDD734 pKa = 3.49TLTNVSGHH742 pKa = 5.9EE743 pKa = 3.98LFEE746 pKa = 4.61VDD748 pKa = 3.9VSSSTLSYY756 pKa = 10.57TFYY759 pKa = 10.71EE760 pKa = 4.17YY761 pKa = 11.17SDD763 pKa = 4.06YY764 pKa = 10.99NTLTNNGEE772 pKa = 4.05MFAFTDD778 pKa = 4.16LAHH781 pKa = 5.62TTSFFEE787 pKa = 4.65LLANTGGTYY796 pKa = 10.44SFHH799 pKa = 7.74LDD801 pKa = 3.39TNSLQSSTTISLTGGAHH818 pKa = 6.56SGTGDD823 pKa = 3.26FLTFVGTTSTFANGNDD839 pKa = 3.4PTTGYY844 pKa = 10.87DD845 pKa = 3.16ILVDD849 pKa = 3.89GWNTADD855 pKa = 4.07TNPNDD860 pKa = 3.62HH861 pKa = 6.19TVHH864 pKa = 5.81VNNNGGGIDD873 pKa = 3.71SAISIPTSPRR883 pKa = 11.84CSGSRR888 pKa = 11.84ITT890 pKa = 5.26

Molecular weight: 88.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B4DCJ5|B4DCJ5_9BACT Tetratricopeptide TPR_4 protein OS=Chthoniobacter flavus Ellin428 OX=497964 GN=CfE428DRAFT_6636 PE=4 SV=1
MM1 pKa = 7.46KK2 pKa = 9.96RR3 pKa = 11.84QLVLRR8 pKa = 11.84VAGRR12 pKa = 11.84AASFLFFIIGTIIAQALRR30 pKa = 11.84VSFSKK35 pKa = 10.69AARR38 pKa = 11.84QRR40 pKa = 11.84RR41 pKa = 11.84ARR43 pKa = 11.84AEE45 pKa = 3.74WLQRR49 pKa = 11.84TAQRR53 pKa = 11.84CVQILRR59 pKa = 11.84LKK61 pKa = 10.21INRR64 pKa = 11.84FGALPTSGLVVANHH78 pKa = 6.86LSYY81 pKa = 10.83IDD83 pKa = 4.41IILLAAQRR91 pKa = 11.84PCVFVSKK98 pKa = 10.82SEE100 pKa = 3.95VHH102 pKa = 5.56SWPIFGQCARR112 pKa = 11.84LGGTIFVDD120 pKa = 4.05RR121 pKa = 11.84KK122 pKa = 10.31HH123 pKa = 6.8RR124 pKa = 11.84GDD126 pKa = 3.38VAGVTDD132 pKa = 4.99LMGAALDD139 pKa = 3.87EE140 pKa = 4.64GALVILFPEE149 pKa = 5.22GTSSGGASVLPFKK162 pKa = 11.09SSLLEE167 pKa = 3.92PALHH171 pKa = 6.21VNHH174 pKa = 7.32PVTAIAIAYY183 pKa = 9.13ALEE186 pKa = 4.19QGSVANEE193 pKa = 3.18ICYY196 pKa = 9.15WRR198 pKa = 11.84DD199 pKa = 3.09MTLLPHH205 pKa = 7.39LLNVWSKK212 pKa = 11.0SLIYY216 pKa = 10.43CSLRR220 pKa = 11.84CGVGRR225 pKa = 11.84QRR227 pKa = 11.84RR228 pKa = 11.84GDD230 pKa = 3.52RR231 pKa = 11.84KK232 pKa = 9.25SLARR236 pKa = 11.84EE237 pKa = 3.78LHH239 pKa = 6.59DD240 pKa = 4.27EE241 pKa = 4.21VAALHH246 pKa = 5.89AASTRR251 pKa = 11.84EE252 pKa = 3.73LAVRR256 pKa = 11.84IPQTFEE262 pKa = 3.42QPRR265 pKa = 11.84PVLMGGLPSAAKK277 pKa = 9.08PQRR280 pKa = 11.84PP281 pKa = 3.4

Molecular weight: 30.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6705

0

6705

2302626

30

8197

343.4

37.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.809 ± 0.035

0.975 ± 0.012

5.223 ± 0.025

5.675 ± 0.05

4.03 ± 0.019

8.196 ± 0.066

2.373 ± 0.022

4.858 ± 0.024

4.069 ± 0.04

9.893 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.0 ± 0.016

3.386 ± 0.056

5.595 ± 0.04

3.594 ± 0.019

6.333 ± 0.054

5.87 ± 0.044

6.173 ± 0.077

6.908 ± 0.025

1.575 ± 0.016

2.466 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski