Sphingomonas jatrophae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3810 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I6KZS2|A0A1I6KZS2_9SPHN Uncharacterized protein OS=Sphingomonas jatrophae OX=1166337 GN=SAMN05192580_2064 PE=4 SV=1
MM1 pKa = 7.85ASPLVRR7 pKa = 11.84FDD9 pKa = 3.94SYY11 pKa = 11.92GSILDD16 pKa = 3.55EE17 pKa = 4.69GAQKK21 pKa = 10.45GRR23 pKa = 11.84PVEE26 pKa = 4.32GFNLTAPPKK35 pKa = 10.05SAAPRR40 pKa = 11.84NVIAADD46 pKa = 3.46ADD48 pKa = 3.85AVVPGSLSGTEE59 pKa = 4.36GYY61 pKa = 10.97DD62 pKa = 3.75LIAVEE67 pKa = 4.94LVAGQTYY74 pKa = 9.98SFAYY78 pKa = 10.1RR79 pKa = 11.84GTPTGGIEE87 pKa = 4.31DD88 pKa = 4.77PYY90 pKa = 11.31LALFDD95 pKa = 4.13TTLSTVLAEE104 pKa = 5.24DD105 pKa = 4.41DD106 pKa = 4.25DD107 pKa = 5.67GGAGRR112 pKa = 11.84SSLITFTAATTGTHH126 pKa = 5.51YY127 pKa = 11.03LYY129 pKa = 10.09ATSFYY134 pKa = 10.56TLDD137 pKa = 3.75TGDD140 pKa = 4.06PSIDD144 pKa = 3.31TGNYY148 pKa = 9.0TIDD151 pKa = 3.08VWSADD156 pKa = 3.78PAKK159 pKa = 10.53DD160 pKa = 3.79APATFAGAEE169 pKa = 4.24LIGAGTTFGFLDD181 pKa = 3.55SAADD185 pKa = 3.37VDD187 pKa = 4.31TYY189 pKa = 11.13RR190 pKa = 11.84IDD192 pKa = 3.69AKK194 pKa = 10.8AGQLYY199 pKa = 8.13TFTYY203 pKa = 10.09AGGIAGSAEE212 pKa = 4.22LADD215 pKa = 5.69PIPGDD220 pKa = 3.85NIGVIDD226 pKa = 3.92IFDD229 pKa = 3.58ARR231 pKa = 11.84GTLVASAVDD240 pKa = 4.1YY241 pKa = 7.46EE242 pKa = 4.51TGASFFAEE250 pKa = 4.11QAGTYY255 pKa = 8.24YY256 pKa = 10.83VQVSPYY262 pKa = 10.53DD263 pKa = 3.55PSLLGGYY270 pKa = 7.37TLDD273 pKa = 3.8VSEE276 pKa = 5.53IDD278 pKa = 3.69PADD281 pKa = 4.14YY282 pKa = 11.12DD283 pKa = 4.15PLDD286 pKa = 4.45SIDD289 pKa = 4.43WRR291 pKa = 11.84DD292 pKa = 3.21ADD294 pKa = 3.73NVPFVDD300 pKa = 3.87VNGVPTAYY308 pKa = 10.54VYY310 pKa = 8.31FAPAGEE316 pKa = 4.44NFGEE320 pKa = 4.35TGDD323 pKa = 4.93DD324 pKa = 3.36GVTPMEE330 pKa = 4.23TFGWQQFQIDD340 pKa = 3.95GVMNALNEE348 pKa = 4.09YY349 pKa = 10.42EE350 pKa = 5.1KK351 pKa = 10.78ILGANYY357 pKa = 9.7EE358 pKa = 4.21ITTDD362 pKa = 3.48PSKK365 pKa = 10.96ATFRR369 pKa = 11.84LLTTEE374 pKa = 4.06SEE376 pKa = 4.27QYY378 pKa = 11.08GAFFYY383 pKa = 10.68PRR385 pKa = 11.84DD386 pKa = 3.69PAYY389 pKa = 9.16GTQQGIGAFNLLSGGFTLPEE409 pKa = 3.97SLQPGGYY416 pKa = 8.52SYY418 pKa = 11.71GVILHH423 pKa = 6.4EE424 pKa = 5.01FGHH427 pKa = 5.85AHH429 pKa = 6.7GLAHH433 pKa = 6.53PHH435 pKa = 6.09DD436 pKa = 4.36TGGGSDD442 pKa = 3.85LMLGVSGPDD451 pKa = 3.12SLGVYY456 pKa = 10.06DD457 pKa = 5.32LNQGVYY463 pKa = 8.91TVMSYY468 pKa = 11.12NDD470 pKa = 3.34GWEE473 pKa = 4.04THH475 pKa = 7.12PDD477 pKa = 3.04GALPFTRR484 pKa = 11.84ATVGYY489 pKa = 8.84GWSGSLSAFDD499 pKa = 3.36IAQLQEE505 pKa = 3.93RR506 pKa = 11.84YY507 pKa = 9.35GVHH510 pKa = 6.19EE511 pKa = 4.59AATGNTTYY519 pKa = 10.89TLDD522 pKa = 4.11DD523 pKa = 3.41VDD525 pKa = 4.11AAGTFYY531 pKa = 10.0QTIWDD536 pKa = 3.82TGGTDD541 pKa = 3.67TIRR544 pKa = 11.84YY545 pKa = 9.38DD546 pKa = 3.43GARR549 pKa = 11.84DD550 pKa = 3.55ARR552 pKa = 11.84IDD554 pKa = 3.82LLAATLDD561 pKa = 3.77YY562 pKa = 10.42TPTGGGVVSYY572 pKa = 11.27VDD574 pKa = 5.44DD575 pKa = 3.28IHH577 pKa = 8.6GGYY580 pKa = 8.83TIANDD585 pKa = 3.65VVIEE589 pKa = 4.03NATGGAGNDD598 pKa = 3.31ALLGNSAKK606 pKa = 10.51NVLTGNAGNDD616 pKa = 3.27ALLGRR621 pKa = 11.84EE622 pKa = 4.38GNDD625 pKa = 2.87RR626 pKa = 11.84LYY628 pKa = 11.33GGAGEE633 pKa = 4.4DD634 pKa = 3.88TLTGGTGLDD643 pKa = 3.98LLFGGAGNDD652 pKa = 3.46VFLMEE657 pKa = 5.47LDD659 pKa = 3.6EE660 pKa = 4.76PANKK664 pKa = 9.92RR665 pKa = 11.84KK666 pKa = 8.44FTLDD670 pKa = 3.42VIGDD674 pKa = 3.93FAKK677 pKa = 10.61GDD679 pKa = 4.07RR680 pKa = 11.84IDD682 pKa = 5.17LSGIDD687 pKa = 3.81ANLNRR692 pKa = 11.84DD693 pKa = 2.99GDD695 pKa = 3.96QAFTMAGKK703 pKa = 9.27FQLLPQIGQIKK714 pKa = 7.66TVSFGSVSAAEE725 pKa = 4.04RR726 pKa = 11.84SLGFDD731 pKa = 4.8LGDD734 pKa = 3.25QKK736 pKa = 11.4AAAGHH741 pKa = 5.78GAVTVLLGEE750 pKa = 4.14VDD752 pKa = 4.63GDD754 pKa = 3.85ILPDD758 pKa = 3.77FAVLMFGSSTVGVSDD773 pKa = 6.13LILL776 pKa = 4.85

Molecular weight: 81.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I6K9R0|A0A1I6K9R0_9SPHN Acetyltransferase (GNAT) domain-containing protein OS=Sphingomonas jatrophae OX=1166337 GN=SAMN05192580_1484 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSRR9 pKa = 11.84LVRR12 pKa = 11.84KK13 pKa = 8.99RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.7GFRR19 pKa = 11.84SRR21 pKa = 11.84SATPGGRR28 pKa = 11.84KK29 pKa = 9.04VLAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84NKK41 pKa = 10.45LSAA44 pKa = 3.94

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3810

0

3810

1249194

29

4164

327.9

35.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.742 ± 0.076

0.721 ± 0.013

5.915 ± 0.033

5.265 ± 0.044

3.321 ± 0.029

9.265 ± 0.062

1.869 ± 0.024

4.454 ± 0.025

2.44 ± 0.033

10.21 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.191 ± 0.023

2.199 ± 0.029

5.523 ± 0.044

2.924 ± 0.023

7.984 ± 0.052

4.757 ± 0.029

5.371 ± 0.053

7.358 ± 0.033

1.412 ± 0.018

2.08 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski