Amino acid dipepetide frequency for Nonomuraea polychroma

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.312AlaAla: 20.312 ± 0.131	1.106AlaCys: 1.106 ± 0.021	7.586AlaAsp: 7.586 ± 0.054	8.32AlaGlu: 8.32 ± 0.059	3.736AlaPhe: 3.736 ± 0.037	12.811AlaGly: 12.811 ± 0.074	2.56AlaHis: 2.56 ± 0.026	4.636AlaIle: 4.636 ± 0.043	3.004AlaLys: 3.004 ± 0.038	13.861AlaLeu: 13.861 ± 0.092	2.841AlaMet: 2.841 ± 0.03	2.152AlaAsn: 2.152 ± 0.03	6.059AlaPro: 6.059 ± 0.048	3.67AlaGln: 3.67 ± 0.036	9.766AlaArg: 9.766 ± 0.059	5.672AlaSer: 5.672 ± 0.046	7.05AlaThr: 7.05 ± 0.052	11.429AlaVal: 11.429 ± 0.07	1.999AlaTrp: 1.999 ± 0.028	2.883AlaTyr: 2.883 ± 0.028	0.0AlaXaa: 0.0 ± 0.0
Cys	0.991CysAla: 0.991 ± 0.018	0.097CysCys: 0.097 ± 0.005	0.486CysAsp: 0.486 ± 0.012	0.412CysGlu: 0.412 ± 0.012	0.226CysPhe: 0.226 ± 0.009	0.924CysGly: 0.924 ± 0.02	0.213CysHis: 0.213 ± 0.007	0.15CysIle: 0.15 ± 0.007	0.121CysLys: 0.121 ± 0.005	0.764CysLeu: 0.764 ± 0.017	0.131CysMet: 0.131 ± 0.007	0.121CysAsn: 0.121 ± 0.005	0.487CysPro: 0.487 ± 0.014	0.171CysGln: 0.171 ± 0.008	0.613CysArg: 0.613 ± 0.014	0.432CysSer: 0.432 ± 0.011	0.464CysThr: 0.464 ± 0.012	0.653CysVal: 0.653 ± 0.014	0.126CysTrp: 0.126 ± 0.006	0.168CysTyr: 0.168 ± 0.007	0.0CysXaa: 0.0 ± 0.0
Asp	6.573AspAla: 6.573 ± 0.05	0.382AspCys: 0.382 ± 0.012	3.492AspAsp: 3.492 ± 0.037	3.579AspGlu: 3.579 ± 0.038	1.604AspPhe: 1.604 ± 0.022	5.942AspGly: 5.942 ± 0.045	1.359AspHis: 1.359 ± 0.022	1.856AspIle: 1.856 ± 0.025	1.209AspLys: 1.209 ± 0.022	6.565AspLeu: 6.565 ± 0.05	0.876AspMet: 0.876 ± 0.016	1.013AspAsn: 1.013 ± 0.019	4.417AspPro: 4.417 ± 0.043	1.715AspGln: 1.715 ± 0.025	4.623AspArg: 4.623 ± 0.035	2.187AspSer: 2.187 ± 0.026	2.7AspThr: 2.7 ± 0.03	5.031AspVal: 5.031 ± 0.044	1.004AspTrp: 1.004 ± 0.018	1.293AspTyr: 1.293 ± 0.023	0.0AspXaa: 0.0 ± 0.0
Glu	6.847GluAla: 6.847 ± 0.058	0.358GluCys: 0.358 ± 0.01	2.576GluAsp: 2.576 ± 0.028	3.426GluGlu: 3.426 ± 0.038	1.491GluPhe: 1.491 ± 0.02	3.922GluGly: 3.922 ± 0.037	1.596GluHis: 1.596 ± 0.024	2.558GluIle: 2.558 ± 0.029	1.198GluLys: 1.198 ± 0.022	6.912GluLeu: 6.912 ± 0.052	0.989GluMet: 0.989 ± 0.019	0.923GluAsn: 0.923 ± 0.019	3.423GluPro: 3.423 ± 0.039	2.265GluGln: 2.265 ± 0.027	5.519GluArg: 5.519 ± 0.053	2.572GluSer: 2.572 ± 0.036	2.732GluThr: 2.732 ± 0.03	4.61GluVal: 4.61 ± 0.046	0.865GluTrp: 0.865 ± 0.018	1.032GluTyr: 1.032 ± 0.016	0.0GluXaa: 0.0 ± 0.0
Phe	3.85PheAla: 3.85 ± 0.034	0.269PheCys: 0.269 ± 0.008	2.079PheAsp: 2.079 ± 0.025	1.47PheGlu: 1.47 ± 0.024	0.949PhePhe: 0.949 ± 0.018	3.098PheGly: 3.098 ± 0.038	0.636PheHis: 0.636 ± 0.014	0.849PheIle: 0.849 ± 0.018	0.554PheLys: 0.554 ± 0.012	2.745PheLeu: 2.745 ± 0.03	0.482PheMet: 0.482 ± 0.013	0.629PheAsn: 0.629 ± 0.015	1.437PhePro: 1.437 ± 0.019	0.754PheGln: 0.754 ± 0.016	1.822PheArg: 1.822 ± 0.027	1.524PheSer: 1.524 ± 0.024	2.096PheThr: 2.096 ± 0.029	2.392PheVal: 2.392 ± 0.03	0.501PheTrp: 0.501 ± 0.011	0.675PheTyr: 0.675 ± 0.015	0.0PheXaa: 0.0 ± 0.0
Gly	10.071GlyAla: 10.071 ± 0.07	0.805GlyCys: 0.805 ± 0.018	5.051GlyAsp: 5.051 ± 0.042	5.071GlyGlu: 5.071 ± 0.04	2.916GlyPhe: 2.916 ± 0.032	8.354GlyGly: 8.354 ± 0.084	2.233GlyHis: 2.233 ± 0.03	3.509GlyIle: 3.509 ± 0.039	2.551GlyLys: 2.551 ± 0.035	9.702GlyLeu: 9.702 ± 0.072	2.121GlyMet: 2.121 ± 0.028	1.806GlyAsn: 1.806 ± 0.026	4.884GlyPro: 4.884 ± 0.039	2.841GlyGln: 2.841 ± 0.037	7.436GlyArg: 7.436 ± 0.054	4.786GlySer: 4.786 ± 0.045	5.643GlyThr: 5.643 ± 0.047	7.703GlyVal: 7.703 ± 0.053	1.783GlyTrp: 1.783 ± 0.023	2.436GlyTyr: 2.436 ± 0.03	0.0GlyXaa: 0.0 ± 0.0
His	2.657HisAla: 2.657 ± 0.031	0.208HisCys: 0.208 ± 0.007	1.392HisAsp: 1.392 ± 0.023	1.203HisGlu: 1.203 ± 0.02	0.601HisPhe: 0.601 ± 0.014	2.22HisGly: 2.22 ± 0.031	0.694HisHis: 0.694 ± 0.016	0.708HisIle: 0.708 ± 0.017	0.354HisLys: 0.354 ± 0.01	2.414HisLeu: 2.414 ± 0.029	0.358HisMet: 0.358 ± 0.01	0.433HisAsn: 0.433 ± 0.013	1.67HisPro: 1.67 ± 0.024	0.651HisGln: 0.651 ± 0.014	1.906HisArg: 1.906 ± 0.023	0.929HisSer: 0.929 ± 0.018	1.159HisThr: 1.159 ± 0.02	1.909HisVal: 1.909 ± 0.025	0.368HisTrp: 0.368 ± 0.012	0.511HisTyr: 0.511 ± 0.014	0.0HisXaa: 0.0 ± 0.0
Ile	5.409IleAla: 5.409 ± 0.047	0.331IleCys: 0.331 ± 0.009	2.645IleAsp: 2.645 ± 0.025	2.341IleGlu: 2.341 ± 0.03	0.953IlePhe: 0.953 ± 0.018	3.874IleGly: 3.874 ± 0.035	0.683IleHis: 0.683 ± 0.014	1.247IleIle: 1.247 ± 0.023	0.964IleLys: 0.964 ± 0.018	2.839IleLeu: 2.839 ± 0.034	0.673IleMet: 0.673 ± 0.014	0.856IleAsn: 0.856 ± 0.015	2.042IlePro: 2.042 ± 0.026	0.874IleGln: 0.874 ± 0.016	2.618IleArg: 2.618 ± 0.032	2.085IleSer: 2.085 ± 0.027	2.526IleThr: 2.526 ± 0.03	3.415IleVal: 3.415 ± 0.037	0.516IleTrp: 0.516 ± 0.013	0.729IleTyr: 0.729 ± 0.017	0.0IleXaa: 0.0 ± 0.0
Lys	2.818LysAla: 2.818 ± 0.034	0.123LysCys: 0.123 ± 0.006	1.24LysAsp: 1.24 ± 0.019	1.244LysGlu: 1.244 ± 0.017	0.473LysPhe: 0.473 ± 0.013	1.843LysGly: 1.843 ± 0.028	0.479LysHis: 0.479 ± 0.013	1.084LysIle: 1.084 ± 0.019	0.712LysLys: 0.712 ± 0.018	2.176LysLeu: 2.176 ± 0.027	0.402LysMet: 0.402 ± 0.012	0.512LysAsn: 0.512 ± 0.012	1.471LysPro: 1.471 ± 0.024	0.744LysGln: 0.744 ± 0.016	1.537LysArg: 1.537 ± 0.021	1.153LysSer: 1.153 ± 0.021	1.353LysThr: 1.353 ± 0.026	2.037LysVal: 2.037 ± 0.03	0.323LysTrp: 0.323 ± 0.011	0.454LysTyr: 0.454 ± 0.011	0.0LysXaa: 0.0 ± 0.0
Leu	15.674LeuAla: 15.674 ± 0.092	0.771LeuCys: 0.771 ± 0.014	6.543LeuAsp: 6.543 ± 0.047	4.973LeuGlu: 4.973 ± 0.046	2.736LeuPhe: 2.736 ± 0.032	9.329LeuGly: 9.329 ± 0.06	2.123LeuHis: 2.123 ± 0.026	4.133LeuIle: 4.133 ± 0.045	2.076LeuLys: 2.076 ± 0.027	11.211LeuLeu: 11.211 ± 0.085	1.852LeuMet: 1.852 ± 0.027	1.852LeuAsn: 1.852 ± 0.027	6.395LeuPro: 6.395 ± 0.048	2.262LeuGln: 2.262 ± 0.026	8.659LeuArg: 8.659 ± 0.057	5.656LeuSer: 5.656 ± 0.044	6.694LeuThr: 6.694 ± 0.043	8.965LeuVal: 8.965 ± 0.065	1.378LeuTrp: 1.378 ± 0.023	1.951LeuTyr: 1.951 ± 0.027	0.0LeuXaa: 0.0 ± 0.0
Met	2.567MetAla: 2.567 ± 0.028	0.124MetCys: 0.124 ± 0.006	0.943MetAsp: 0.943 ± 0.017	0.87MetGlu: 0.87 ± 0.017	0.543MetPhe: 0.543 ± 0.013	1.441MetGly: 1.441 ± 0.021	0.373MetHis: 0.373 ± 0.011	0.948MetIle: 0.948 ± 0.017	0.472MetLys: 0.472 ± 0.011	2.02MetLeu: 2.02 ± 0.029	0.353MetMet: 0.353 ± 0.012	0.495MetAsn: 0.495 ± 0.013	1.237MetPro: 1.237 ± 0.018	0.451MetGln: 0.451 ± 0.012	1.736MetArg: 1.736 ± 0.024	1.384MetSer: 1.384 ± 0.021	1.581MetThr: 1.581 ± 0.024	1.497MetVal: 1.497 ± 0.019	0.253MetTrp: 0.253 ± 0.009	0.336MetTyr: 0.336 ± 0.01	0.0MetXaa: 0.0 ± 0.0
Asn	2.284AsnAla: 2.284 ± 0.031	0.166AsnCys: 0.166 ± 0.008	1.02AsnAsp: 1.02 ± 0.018	0.887AsnGlu: 0.887 ± 0.019	0.539AsnPhe: 0.539 ± 0.013	2.023AsnGly: 2.023 ± 0.032	0.405AsnHis: 0.405 ± 0.009	0.702AsnIle: 0.702 ± 0.014	0.435AsnLys: 0.435 ± 0.013	1.864AsnLeu: 1.864 ± 0.026	0.322AsnMet: 0.322 ± 0.01	0.475AsnAsn: 0.475 ± 0.016	1.516AsnPro: 1.516 ± 0.023	0.56AsnGln: 0.56 ± 0.014	1.349AsnArg: 1.349 ± 0.021	0.875AsnSer: 0.875 ± 0.018	1.11AsnThr: 1.11 ± 0.02	1.627AsnVal: 1.627 ± 0.027	0.322AsnTrp: 0.322 ± 0.011	0.435AsnTyr: 0.435 ± 0.013	0.0AsnXaa: 0.0 ± 0.0
Pro	8.077ProAla: 8.077 ± 0.057	0.347ProCys: 0.347 ± 0.012	4.223ProAsp: 4.223 ± 0.039	4.09ProGlu: 4.09 ± 0.04	1.655ProPhe: 1.655 ± 0.02	6.379ProGly: 6.379 ± 0.049	1.314ProHis: 1.314 ± 0.021	1.896ProIle: 1.896 ± 0.021	1.271ProLys: 1.271 ± 0.024	5.346ProLeu: 5.346 ± 0.045	1.164ProMet: 1.164 ± 0.02	1.003ProAsn: 1.003 ± 0.018	3.704ProPro: 3.704 ± 0.05	1.653ProGln: 1.653 ± 0.026	3.732ProArg: 3.732 ± 0.043	3.266ProSer: 3.266 ± 0.036	3.099ProThr: 3.099 ± 0.039	5.124ProVal: 5.124 ± 0.046	0.986ProTrp: 0.986 ± 0.019	1.531ProTyr: 1.531 ± 0.024	0.0ProXaa: 0.0 ± 0.0
Gln	4.276GlnAla: 4.276 ± 0.042	0.168GlnCys: 0.168 ± 0.007	1.331GlnAsp: 1.331 ± 0.023	1.474GlnGlu: 1.474 ± 0.023	0.729GlnPhe: 0.729 ± 0.015	2.275GlnGly: 2.275 ± 0.028	0.634GlnHis: 0.634 ± 0.013	1.23GlnIle: 1.23 ± 0.019	0.558GlnLys: 0.558 ± 0.015	2.87GlnLeu: 2.87 ± 0.029	0.525GlnMet: 0.525 ± 0.014	0.515GlnAsn: 0.515 ± 0.014	1.838GlnPro: 1.838 ± 0.028	1.149GlnGln: 1.149 ± 0.027	2.411GlnArg: 2.411 ± 0.028	1.268GlnSer: 1.268 ± 0.023	1.395GlnThr: 1.395 ± 0.022	2.66GlnVal: 2.66 ± 0.03	0.508GlnTrp: 0.508 ± 0.012	0.541GlnTyr: 0.541 ± 0.013	0.0GlnXaa: 0.0 ± 0.0
Arg	9.161ArgAla: 9.161 ± 0.063	0.57ArgCys: 0.57 ± 0.014	4.262ArgAsp: 4.262 ± 0.041	4.606ArgGlu: 4.606 ± 0.045	2.44ArgPhe: 2.44 ± 0.029	5.518ArgGly: 5.518 ± 0.043	2.159ArgHis: 2.159 ± 0.022	3.222ArgIle: 3.222 ± 0.034	1.702ArgLys: 1.702 ± 0.025	9.412ArgLeu: 9.412 ± 0.062	1.923ArgMet: 1.923 ± 0.021	1.412ArgAsn: 1.412 ± 0.024	4.8ArgPro: 4.8 ± 0.042	2.514ArgGln: 2.514 ± 0.029	7.706ArgArg: 7.706 ± 0.066	3.816ArgSer: 3.816 ± 0.037	4.568ArgThr: 4.568 ± 0.039	6.072ArgVal: 6.072 ± 0.041	1.477ArgTrp: 1.477 ± 0.022	1.919ArgTyr: 1.919 ± 0.025	0.0ArgXaa: 0.0 ± 0.0
Ser	6.265SerAla: 6.265 ± 0.054	0.412SerCys: 0.412 ± 0.012	2.585SerAsp: 2.585 ± 0.029	2.35SerGlu: 2.35 ± 0.029	1.647SerPhe: 1.647 ± 0.025	5.736SerGly: 5.736 ± 0.048	1.006SerHis: 1.006 ± 0.016	1.839SerIle: 1.839 ± 0.024	1.104SerLys: 1.104 ± 0.018	4.761SerLeu: 4.761 ± 0.043	1.228SerMet: 1.228 ± 0.019	0.91SerAsn: 0.91 ± 0.019	3.261SerPro: 3.261 ± 0.032	1.302SerGln: 1.302 ± 0.02	3.768SerArg: 3.768 ± 0.037	2.82SerSer: 2.82 ± 0.031	2.966SerThr: 2.966 ± 0.035	4.085SerVal: 4.085 ± 0.034	1.012SerTrp: 1.012 ± 0.021	1.208SerTyr: 1.208 ± 0.021	0.0SerXaa: 0.0 ± 0.0
Thr	7.786ThrAla: 7.786 ± 0.058	0.475ThrCys: 0.475 ± 0.015	2.967ThrAsp: 2.967 ± 0.034	2.759ThrGlu: 2.759 ± 0.029	1.838ThrPhe: 1.838 ± 0.025	6.185ThrGly: 6.185 ± 0.054	1.141ThrHis: 1.141 ± 0.019	2.304ThrIle: 2.304 ± 0.028	1.236ThrLys: 1.236 ± 0.019	5.931ThrLeu: 5.931 ± 0.05	1.165ThrMet: 1.165 ± 0.018	1.045ThrAsn: 1.045 ± 0.021	3.975ThrPro: 3.975 ± 0.043	1.379ThrGln: 1.379 ± 0.022	3.883ThrArg: 3.883 ± 0.035	3.174ThrSer: 3.174 ± 0.033	3.78ThrThr: 3.78 ± 0.047	5.593ThrVal: 5.593 ± 0.045	1.054ThrTrp: 1.054 ± 0.02	1.388ThrTyr: 1.388 ± 0.028	0.0ThrXaa: 0.0 ± 0.0
Val	11.338ValAla: 11.338 ± 0.069	0.683ValCys: 0.683 ± 0.015	4.632ValAsp: 4.632 ± 0.039	4.735ValGlu: 4.735 ± 0.039	2.54ValPhe: 2.54 ± 0.03	6.376ValGly: 6.376 ± 0.056	1.816ValHis: 1.816 ± 0.025	3.492ValIle: 3.492 ± 0.036	1.89ValLys: 1.89 ± 0.026	9.525ValLeu: 9.525 ± 0.063	1.568ValMet: 1.568 ± 0.021	1.793ValAsn: 1.793 ± 0.029	5.2ValPro: 5.2 ± 0.042	2.071ValGln: 2.071 ± 0.024	6.738ValArg: 6.738 ± 0.056	4.64ValSer: 4.64 ± 0.041	5.813ValThr: 5.813 ± 0.048	7.983ValVal: 7.983 ± 0.075	1.194ValTrp: 1.194 ± 0.021	1.721ValTyr: 1.721 ± 0.022	0.0ValXaa: 0.0 ± 0.0
Trp	1.768TrpAla: 1.768 ± 0.024	0.164TrpCys: 0.164 ± 0.007	0.871TrpAsp: 0.871 ± 0.019	0.769TrpGlu: 0.769 ± 0.014	0.515TrpPhe: 0.515 ± 0.013	1.098TrpGly: 1.098 ± 0.018	0.447TrpHis: 0.447 ± 0.012	0.641TrpIle: 0.641 ± 0.014	0.359TrpLys: 0.359 ± 0.012	1.986TrpLeu: 1.986 ± 0.028	0.344TrpMet: 0.344 ± 0.011	0.489TrpAsn: 0.489 ± 0.013	0.905TrpPro: 0.905 ± 0.019	0.637TrpGln: 0.637 ± 0.013	1.537TrpArg: 1.537 ± 0.021	1.026TrpSer: 1.026 ± 0.019	1.045TrpThr: 1.045 ± 0.02	1.062TrpVal: 1.062 ± 0.018	0.392TrpTrp: 0.392 ± 0.011	0.397TrpTyr: 0.397 ± 0.013	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.729TyrAla: 2.729 ± 0.028	0.191TyrCys: 0.191 ± 0.007	1.493TyrAsp: 1.493 ± 0.03	1.236TyrGlu: 1.236 ± 0.023	0.678TyrPhe: 0.678 ± 0.014	2.354TyrGly: 2.354 ± 0.033	0.475TyrHis: 0.475 ± 0.013	0.612TyrIle: 0.612 ± 0.015	0.408TyrLys: 0.408 ± 0.013	2.331TyrLeu: 2.331 ± 0.028	0.307TyrMet: 0.307 ± 0.01	0.483TyrAsn: 0.483 ± 0.014	1.142TyrPro: 1.142 ± 0.018	0.691TyrGln: 0.691 ± 0.017	1.901TyrArg: 1.901 ± 0.028	0.998TyrSer: 0.998 ± 0.017	1.288TyrThr: 1.288 ± 0.023	1.901TyrVal: 1.901 ± 0.023	0.389TyrTrp: 0.389 ± 0.012	0.534TyrTyr: 0.534 ± 0.016	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 10251 proteins (3320502 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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