Bradyrhizobium sp. Leo170

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Bradyrhizobiaceae; Bradyrhizobium; unclassified Bradyrhizobium

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8497 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q8R0Z7|A0A4Q8R0Z7_9BRAD CBS domain-containing protein OS=Bradyrhizobium sp. Leo170 OX=1571199 GN=CWO89_05715 PE=4 SV=1
MM1 pKa = 7.65RR2 pKa = 11.84FSLAAAILLPALLTANAAVAQVTGMATPTPTLDD35 pKa = 3.11ATSPLGSASSPVSPTGIPLGATEE58 pKa = 4.91LSSAGVSPLAAPATGTIAIPSSGTVCSTVGTAPTQMFGSTATFDD102 pKa = 3.86GAGMAVGSTAPATAAMAGDD121 pKa = 4.2TAATPGMSPAISATSGVSTTSGTLEE146 pKa = 3.95SSGMATASGIIDD158 pKa = 3.54TSGTSGMCGSGSTSVIASSTPSSTSPTTVGGVQRR192 pKa = 11.84TGIPLDD198 pKa = 3.41SSEE201 pKa = 3.93ISNLGVSSGAPVPTPGVSLLPGVVGPVAALPTVPTVLAPTPTASTMAGSSACSGFVTGSVTSSAVIPGCC270 pKa = 4.5

Molecular weight: 24.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q8R647|A0A4Q8R647_9BRAD Uncharacterized protein OS=Bradyrhizobium sp. Leo170 OX=1571199 GN=CWO89_07380 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.55GFRR19 pKa = 11.84ARR21 pKa = 11.84LATAGGRR28 pKa = 11.84KK29 pKa = 8.95VLAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8497

0

8497

2502016

25

4210

294.5

31.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.192 ± 0.033

0.873 ± 0.01

5.484 ± 0.021

5.35 ± 0.028

3.767 ± 0.018

8.358 ± 0.039

2.017 ± 0.015

5.422 ± 0.018

3.582 ± 0.025

9.895 ± 0.03

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.373 ± 0.013

2.851 ± 0.02

5.248 ± 0.019

3.234 ± 0.015

7.056 ± 0.031

5.785 ± 0.026

5.416 ± 0.026

7.442 ± 0.022

1.342 ± 0.012

2.312 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski