Beihai sobemo-like virus 24

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KE68|A0A1L3KE68_9VIRU RNA-directed RNA polymerase OS=Beihai sobemo-like virus 24 OX=1922696 PE=4 SV=1
MM1 pKa = 7.54EE2 pKa = 6.19PIEE5 pKa = 4.77MITTVLGTLVLWEE18 pKa = 4.04IVRR21 pKa = 11.84SLFRR25 pKa = 11.84LLAQRR30 pKa = 11.84FRR32 pKa = 11.84RR33 pKa = 11.84VNVTGKK39 pKa = 8.07TLEE42 pKa = 4.38DD43 pKa = 3.51YY44 pKa = 11.04QLIGEE49 pKa = 4.53DD50 pKa = 3.75VACRR54 pKa = 11.84RR55 pKa = 11.84VYY57 pKa = 11.3KK58 pKa = 8.36MTKK61 pKa = 9.83SDD63 pKa = 3.74FQFEE67 pKa = 4.71SIRR70 pKa = 11.84KK71 pKa = 8.21NSKK74 pKa = 10.42LEE76 pKa = 3.94EE77 pKa = 4.25TKK79 pKa = 10.62SIHH82 pKa = 5.8QDD84 pKa = 3.05QVALLDD90 pKa = 3.95KK91 pKa = 10.22EE92 pKa = 4.72GTFIGYY98 pKa = 9.01GIRR101 pKa = 11.84FEE103 pKa = 5.17AGLVLPHH110 pKa = 6.67HH111 pKa = 6.99VWQCARR117 pKa = 11.84NGFLIGPTGKK127 pKa = 9.68QVAMEE132 pKa = 4.42GKK134 pKa = 8.51LTIISTDD141 pKa = 3.53LAIVDD146 pKa = 4.51IPNKK150 pKa = 9.68SWSTLGVKK158 pKa = 7.56VTKK161 pKa = 9.78PIPVAGNTFAEE172 pKa = 4.49ASGNPLLDD180 pKa = 4.89DD181 pKa = 4.13GLVRR185 pKa = 11.84LQGHH189 pKa = 6.44RR190 pKa = 11.84SFGYY194 pKa = 10.49LEE196 pKa = 4.22PASFGMLHH204 pKa = 6.14YY205 pKa = 10.69GGSTRR210 pKa = 11.84RR211 pKa = 11.84GMSGCGYY218 pKa = 10.59YY219 pKa = 10.64VGDD222 pKa = 4.32KK223 pKa = 10.79LAGLHH228 pKa = 6.32IGGGLVNHH236 pKa = 7.49GYY238 pKa = 9.8SAAFIQAITSHH249 pKa = 6.39GEE251 pKa = 3.79STEE254 pKa = 5.01DD255 pKa = 3.59YY256 pKa = 11.23LLDD259 pKa = 3.45LVKK262 pKa = 10.38RR263 pKa = 11.84RR264 pKa = 11.84KK265 pKa = 9.77RR266 pKa = 11.84GLDD269 pKa = 3.23YY270 pKa = 11.04RR271 pKa = 11.84RR272 pKa = 11.84VPGLLGEE279 pKa = 4.46VAVCLPGGRR288 pKa = 11.84FTIMEE293 pKa = 4.33EE294 pKa = 3.83EE295 pKa = 4.37DD296 pKa = 3.65ALEE299 pKa = 4.35LGILGDD305 pKa = 4.34EE306 pKa = 4.48LFKK309 pKa = 11.31SPEE312 pKa = 4.01GAVSYY317 pKa = 10.5EE318 pKa = 3.95DD319 pKa = 3.98QVLKK323 pKa = 10.14MKK325 pKa = 10.6NVEE328 pKa = 4.15IPSDD332 pKa = 3.35PTEE335 pKa = 3.57AHH337 pKa = 5.94FVKK340 pKa = 10.47EE341 pKa = 4.0VLDD344 pKa = 4.19AGGEE348 pKa = 4.1AEE350 pKa = 4.78TIGDD354 pKa = 4.37LDD356 pKa = 3.97SVFQKK361 pKa = 10.96RR362 pKa = 11.84LGDD365 pKa = 3.56QAQKK369 pKa = 10.94LRR371 pKa = 11.84DD372 pKa = 3.69EE373 pKa = 4.7LKK375 pKa = 10.63AIQEE379 pKa = 4.02QRR381 pKa = 11.84AKK383 pKa = 10.63EE384 pKa = 4.15SVRR387 pKa = 11.84PKK389 pKa = 10.07VQPSDD394 pKa = 3.4TTLLEE399 pKa = 4.19SQISSLKK406 pKa = 9.62EE407 pKa = 3.3QLKK410 pKa = 10.62AIRR413 pKa = 11.84LEE415 pKa = 4.22DD416 pKa = 3.27QQQRR420 pKa = 11.84EE421 pKa = 4.19KK422 pKa = 10.9QKK424 pKa = 10.95QEE426 pKa = 3.64VQADD430 pKa = 3.91RR431 pKa = 11.84EE432 pKa = 4.44YY433 pKa = 10.92QQSLLEE439 pKa = 4.23KK440 pKa = 10.1KK441 pKa = 10.13KK442 pKa = 10.4QLEE445 pKa = 4.21KK446 pKa = 10.63EE447 pKa = 4.11LASVSVLASSRR458 pKa = 11.84NYY460 pKa = 8.75LTKK463 pKa = 10.36QSKK466 pKa = 10.59SSLKK470 pKa = 10.16RR471 pKa = 11.84EE472 pKa = 3.68KK473 pKa = 10.83ARR475 pKa = 11.84AKK477 pKa = 10.5KK478 pKa = 8.36MQEE481 pKa = 3.96EE482 pKa = 4.06AQHH485 pKa = 6.26AKK487 pKa = 9.22VAKK490 pKa = 9.77EE491 pKa = 3.76LEE493 pKa = 4.35EE494 pKa = 3.96LRR496 pKa = 11.84KK497 pKa = 9.69LVPTLGTKK505 pKa = 8.9SS506 pKa = 3.19

Molecular weight: 56.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KE68|A0A1L3KE68_9VIRU RNA-directed RNA polymerase OS=Beihai sobemo-like virus 24 OX=1922696 PE=4 SV=1
MM1 pKa = 7.84DD2 pKa = 3.69WDD4 pKa = 3.93IYY6 pKa = 11.13RR7 pKa = 11.84KK8 pKa = 9.3ILEE11 pKa = 4.77EE12 pKa = 4.18IDD14 pKa = 3.65PKK16 pKa = 10.89KK17 pKa = 11.0SPGYY21 pKa = 9.12PFKK24 pKa = 11.05AWGITTNKK32 pKa = 10.02QILDD36 pKa = 3.74SSKK39 pKa = 10.37MLVQLYY45 pKa = 10.58CMVRR49 pKa = 11.84QRR51 pKa = 11.84LDD53 pKa = 2.9QLARR57 pKa = 11.84GRR59 pKa = 11.84VGGDD63 pKa = 4.03DD64 pKa = 3.07INLFIKK70 pKa = 10.36QEE72 pKa = 3.82PHH74 pKa = 6.49KK75 pKa = 10.38KK76 pKa = 9.98SKK78 pKa = 10.55AAAKK82 pKa = 9.68RR83 pKa = 11.84WRR85 pKa = 11.84LISGVGLVDD94 pKa = 4.32NIVDD98 pKa = 3.67RR99 pKa = 11.84FLFGGLLEE107 pKa = 4.51KK108 pKa = 10.5VDD110 pKa = 4.22YY111 pKa = 10.09LAKK114 pKa = 10.39RR115 pKa = 11.84MKK117 pKa = 10.34IPILAGWVPWGGGYY131 pKa = 9.71RR132 pKa = 11.84ALANTIQEE140 pKa = 4.26PQSCDD145 pKa = 2.81KK146 pKa = 11.22SAWDD150 pKa = 3.26WTMQGWIVQGFQQILRR166 pKa = 11.84TIQCNPDD173 pKa = 2.56WSLAIDD179 pKa = 3.65ARR181 pKa = 11.84LATLFNASVFRR192 pKa = 11.84VGEE195 pKa = 3.88RR196 pKa = 11.84RR197 pKa = 11.84FKK199 pKa = 10.45QNVMGIMKK207 pKa = 10.21SGFLGTIVFNSIGQMLIHH225 pKa = 6.59HH226 pKa = 7.07LSLLRR231 pKa = 11.84GKK233 pKa = 10.15GKK235 pKa = 9.88RR236 pKa = 11.84LPYY239 pKa = 10.1FVVGDD244 pKa = 3.87DD245 pKa = 3.79MVTEE249 pKa = 4.35KK250 pKa = 9.49QTPAYY255 pKa = 8.02WSAVEE260 pKa = 4.18TSGCSLKK267 pKa = 10.49EE268 pKa = 3.95YY269 pKa = 9.64QEE271 pKa = 4.27KK272 pKa = 10.23PGYY275 pKa = 7.29PTEE278 pKa = 4.04FMGVKK283 pKa = 10.43FSEE286 pKa = 4.16NVILPAYY293 pKa = 8.33PDD295 pKa = 3.43KK296 pKa = 11.8NMFSLLIKK304 pKa = 10.17EE305 pKa = 4.14PEE307 pKa = 3.86ILKK310 pKa = 10.28EE311 pKa = 4.12ALRR314 pKa = 11.84CYY316 pKa = 9.96QLLYY320 pKa = 9.92SHH322 pKa = 7.06HH323 pKa = 7.2PLRR326 pKa = 11.84SVVADD331 pKa = 3.77LAKK334 pKa = 10.37AYY336 pKa = 8.38EE337 pKa = 4.23VQLPEE342 pKa = 4.31SEE344 pKa = 4.35VVSWVEE350 pKa = 3.75GWEE353 pKa = 4.34CAA355 pKa = 4.38

Molecular weight: 40.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

861

355

506

430.5

48.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.388 ± 0.115

1.161 ± 0.318

4.994 ± 0.046

7.666 ± 1.221

3.368 ± 0.346

8.13 ± 0.315

1.742 ± 0.201

5.459 ± 0.613

8.014 ± 0.245

11.034 ± 0.537

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.323 ± 0.466

2.207 ± 0.367

3.833 ± 0.575

5.575 ± 0.303

5.807 ± 0.274

6.388 ± 0.284

4.181 ± 0.651

6.969 ± 0.044

1.742 ± 0.985

3.02 ± 0.386
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski