Lactococcus phage AM3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Audreyjarvisvirus; unclassified Audreyjarvisvirus

Average proteome isoelectric point is 5.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 177 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W6JK51|A0A1W6JK51_9CAUD Uncharacterized protein OS=Lactococcus phage AM3 OX=1965471 GN=AM3_134 PE=4 SV=1
MM1 pKa = 7.7RR2 pKa = 11.84FKK4 pKa = 11.07AKK6 pKa = 10.12PKK8 pKa = 10.53EE9 pKa = 4.03EE10 pKa = 4.13LTEE13 pKa = 4.03EE14 pKa = 4.31TVDD17 pKa = 3.21EE18 pKa = 4.46DD19 pKa = 2.87LWYY22 pKa = 10.34FLKK25 pKa = 10.67EE26 pKa = 4.14YY27 pKa = 10.15IDD29 pKa = 4.37DD30 pKa = 3.72EE31 pKa = 5.1GYY33 pKa = 10.22IYY35 pKa = 10.84GWYY38 pKa = 8.85VDD40 pKa = 4.01GYY42 pKa = 9.46IVGGMVEE49 pKa = 4.76ANEE52 pKa = 4.77EE53 pKa = 4.42YY54 pKa = 10.56CSLEE58 pKa = 3.79YY59 pKa = 9.6WVSVIKK65 pKa = 10.09DD66 pKa = 3.4TVVLVEE72 pKa = 4.12EE73 pKa = 4.52

Molecular weight: 8.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W6JK07|A0A1W6JK07_9CAUD Uncharacterized protein OS=Lactococcus phage AM3 OX=1965471 GN=AM3_082 PE=4 SV=1
MM1 pKa = 7.08WRR3 pKa = 11.84DD4 pKa = 3.28IIVEE8 pKa = 4.1KK9 pKa = 10.94KK10 pKa = 9.37EE11 pKa = 3.42VTMRR15 pKa = 11.84VGEE18 pKa = 3.88INMFSKK24 pKa = 8.9HH25 pKa = 5.51TYY27 pKa = 7.93PHH29 pKa = 6.69PVDD32 pKa = 3.47NLRR35 pKa = 11.84KK36 pKa = 9.88NRR38 pKa = 11.84IFYY41 pKa = 9.93AKK43 pKa = 10.16LWITHH48 pKa = 5.86RR49 pKa = 11.84SKK51 pKa = 10.68IRR53 pKa = 11.84ILLLHH58 pKa = 6.81ISAHH62 pKa = 5.76SRR64 pKa = 11.84TFLDD68 pKa = 3.4NKK70 pKa = 8.21PLKK73 pKa = 9.36WLIRR77 pKa = 11.84EE78 pKa = 4.42FFMM81 pKa = 6.61

Molecular weight: 10.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

177

0

177

35912

29

3205

202.9

23.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.834 ± 0.338

0.699 ± 0.098

6.889 ± 0.177

8.117 ± 0.25

4.547 ± 0.193

5.714 ± 0.22

1.52 ± 0.104

7.666 ± 0.182

9.289 ± 0.213

8.12 ± 0.19

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.659 ± 0.114

6.499 ± 0.165

2.225 ± 0.114

3.403 ± 0.284

3.489 ± 0.159

6.519 ± 0.249

6.001 ± 0.214

6.265 ± 0.161

1.025 ± 0.079

4.519 ± 0.2
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski