Micromonospora olivasterospora

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micromonosporales; Micromonosporaceae; Micromo

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6202 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A562IHQ9|A0A562IHQ9_MICOL Uncharacterized protein OS=Micromonospora olivasterospora OX=1880 GN=JD77_05381 PE=4 SV=1
MM1 pKa = 7.73IDD3 pKa = 3.83DD4 pKa = 5.42GYY6 pKa = 11.45FGDD9 pKa = 4.49EE10 pKa = 3.67VAARR14 pKa = 11.84YY15 pKa = 8.59DD16 pKa = 3.76DD17 pKa = 3.96PTSEE21 pKa = 3.92MFAPNVVGATVDD33 pKa = 3.52VLAEE37 pKa = 4.0LAGPWSWASAPDD49 pKa = 3.58ASRR52 pKa = 11.84CRR54 pKa = 11.84SPAAAYY60 pKa = 10.15LVFNTIMNLTTT71 pKa = 3.75

Molecular weight: 7.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A562I6M7|A0A562I6M7_MICOL Serine protease OS=Micromonospora olivasterospora OX=1880 GN=JD77_01635 PE=3 SV=1
MM1 pKa = 7.47TSPMPPPSAWPAPSTWPAAAARR23 pKa = 11.84STAAAPTTGAPAGWPRR39 pKa = 11.84PSASRR44 pKa = 11.84CPTPPPSRR52 pKa = 11.84SRR54 pKa = 11.84PPMSPMSGMPPGPPTRR70 pKa = 11.84RR71 pKa = 11.84RR72 pKa = 11.84TTGRR76 pKa = 11.84RR77 pKa = 11.84APAGAGAARR86 pKa = 11.84RR87 pKa = 11.84AARR90 pKa = 11.84PTPPRR95 pKa = 11.84PTGTGQRR102 pKa = 11.84PRR104 pKa = 11.84THH106 pKa = 6.78RR107 pKa = 11.84PPRR110 pKa = 11.84RR111 pKa = 11.84GWPLRR116 pKa = 11.84SRR118 pKa = 11.84GRR120 pKa = 11.84RR121 pKa = 11.84RR122 pKa = 11.84VPPSNRR128 pKa = 11.84PRR130 pKa = 11.84SRR132 pKa = 11.84GRR134 pKa = 11.84KK135 pKa = 7.16PHH137 pKa = 6.64PLPGRR142 pKa = 11.84KK143 pKa = 8.83RR144 pKa = 11.84LRR146 pKa = 11.84PRR148 pKa = 11.84ASRR151 pKa = 11.84RR152 pKa = 11.84LPPCGTSRR160 pKa = 11.84LRR162 pKa = 11.84PCGTSRR168 pKa = 11.84SRR170 pKa = 11.84SRR172 pKa = 11.84GVGRR176 pKa = 11.84TPASRR181 pKa = 11.84PPRR184 pKa = 11.84GRR186 pKa = 11.84SGPGSRR192 pKa = 11.84RR193 pKa = 11.84TPPRR197 pKa = 11.84PVRR200 pKa = 11.84LAPAQPWRR208 pKa = 11.84IRR210 pKa = 11.84PPPTRR215 pKa = 11.84PAPTRR220 pKa = 11.84PGRR223 pKa = 11.84APRR226 pKa = 11.84WPTRR230 pKa = 11.84PPPCRR235 pKa = 11.84RR236 pKa = 11.84AVRR239 pKa = 11.84PVGAAPASGRR249 pKa = 11.84AGPQPTPPRR258 pKa = 11.84PPGRR262 pKa = 11.84TPARR266 pKa = 11.84ARR268 pKa = 11.84PARR271 pKa = 11.84RR272 pKa = 11.84PRR274 pKa = 3.62

Molecular weight: 29.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6202

0

6202

2002684

39

8903

322.9

34.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.171 ± 0.063

0.774 ± 0.012

6.035 ± 0.029

5.081 ± 0.032

2.532 ± 0.019

9.49 ± 0.032

2.142 ± 0.015

2.979 ± 0.02

1.68 ± 0.026

10.234 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.601 ± 0.012

1.722 ± 0.023

6.754 ± 0.038

2.817 ± 0.027

8.844 ± 0.043

4.746 ± 0.03

5.922 ± 0.031

8.845 ± 0.037

1.663 ± 0.014

1.969 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski