Humibacillus sp. DSM 29435

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Intrasporangiaceae; Humibacillus; unclassified Humibacillus

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4370 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1E8CY35|A0A1E8CY35_9MICO Phenazine biosynthesis protein PhzF family OS=Humibacillus sp. DSM 29435 OX=1869167 GN=BA895_16380 PE=4 SV=1
MM1 pKa = 7.85SDD3 pKa = 4.27LDD5 pKa = 3.93YY6 pKa = 11.24PDD8 pKa = 4.63DD9 pKa = 3.87LRR11 pKa = 11.84YY12 pKa = 7.11TTDD15 pKa = 3.41HH16 pKa = 5.19EE17 pKa = 4.81WIRR20 pKa = 11.84VGADD24 pKa = 2.78GMVRR28 pKa = 11.84IGITAFAQDD37 pKa = 3.34ALGDD41 pKa = 3.82VVYY44 pKa = 11.1VSLPAAGEE52 pKa = 4.22TVAVGDD58 pKa = 3.53ACGEE62 pKa = 4.21VEE64 pKa = 4.37STKK67 pKa = 10.76SVSDD71 pKa = 3.43VYY73 pKa = 11.43APLEE77 pKa = 4.37GEE79 pKa = 4.36VTAVNPALDD88 pKa = 3.95ATPEE92 pKa = 4.79LINSDD97 pKa = 3.97PYY99 pKa = 10.96GEE101 pKa = 3.82GWMFEE106 pKa = 4.14MKK108 pKa = 10.56LADD111 pKa = 3.71SSALEE116 pKa = 3.88ALMDD120 pKa = 3.98SEE122 pKa = 5.51TYY124 pKa = 9.76QASLTT129 pKa = 3.93

Molecular weight: 13.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1E8CPC8|A0A1E8CPC8_9MICO Uncharacterized protein OS=Humibacillus sp. DSM 29435 OX=1869167 GN=BA895_10100 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84SILAGRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.83GRR40 pKa = 11.84AEE42 pKa = 3.84LSAA45 pKa = 4.93

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4370

0

4370

1425508

28

2471

326.2

34.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.027 ± 0.047

0.716 ± 0.01

6.178 ± 0.033

5.097 ± 0.034

2.861 ± 0.023

9.254 ± 0.03

2.254 ± 0.022

3.685 ± 0.03

1.986 ± 0.03

10.163 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.827 ± 0.014

1.914 ± 0.022

5.641 ± 0.03

2.901 ± 0.019

7.471 ± 0.043

5.844 ± 0.026

6.331 ± 0.037

9.41 ± 0.036

1.564 ± 0.014

1.876 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski