Piromyces sp. (strain E2)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Fungi incertae sedis; Chytridiomycota; Chytridiomycota incertae sedis; Neocallimastigomycetes; Neocallimastigales; Neocallimastigaceae; Piromyces; unclassified Piromyces

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14606 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y3MUR2|A0A1Y3MUR2_PIRSE SHSP domain-containing protein (Fragment) OS=Piromyces sp. (strain E2) OX=73868 GN=PIROE2DRAFT_29606 PE=3 SV=1
MM1 pKa = 7.49GNDD4 pKa = 3.06ISNGISDD11 pKa = 4.77NMNNDD16 pKa = 2.98SNHH19 pKa = 5.24YY20 pKa = 10.43VNSNMNNTFSNNVNNDD36 pKa = 2.69KK37 pKa = 11.21SKK39 pKa = 11.18DD40 pKa = 3.38IIFEE44 pKa = 3.92IGIMVYY50 pKa = 10.46VDD52 pKa = 3.48ADD54 pKa = 3.68YY55 pKa = 11.58AGDD58 pKa = 4.41LKK60 pKa = 10.83IRR62 pKa = 11.84CNNINNVMNNTFSNNVNNDD81 pKa = 2.84MSNAFSNNISYY92 pKa = 10.82NVDD95 pKa = 3.13NNVINVYY102 pKa = 10.53SNNVMDD108 pKa = 5.26LNHH111 pKa = 6.72VIYY114 pKa = 11.18NNIDD118 pKa = 3.42NNFSNISGNGNSSNKK133 pKa = 8.22ITVNGQEE140 pKa = 4.19FNVDD144 pKa = 3.13EE145 pKa = 4.79SFDD148 pKa = 3.61GSFDD152 pKa = 3.38ILYY155 pKa = 10.43VLYY158 pKa = 10.74DD159 pKa = 3.76GSTVGFINDD168 pKa = 4.28AIIYY172 pKa = 8.82RR173 pKa = 11.84FQILNQKK180 pKa = 9.54NEE182 pKa = 3.86NSTEE186 pKa = 3.94EE187 pKa = 4.34LDD189 pKa = 4.42NISSSSIPEE198 pKa = 3.87VPITPEE204 pKa = 3.91TPSTSFTPIHH214 pKa = 6.58FFPKK218 pKa = 8.45VTVSAEE224 pKa = 3.79IQAEE228 pKa = 4.44SVTTMEE234 pKa = 4.11MVIINGISS242 pKa = 2.8

Molecular weight: 26.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y3N307|A0A1Y3N307_PIRSE U3 small nucleolar RNA-associated protein 22 OS=Piromyces sp. (strain E2) OX=73868 GN=PIROE2DRAFT_69533 PE=3 SV=1
PP1 pKa = 7.11SNKK4 pKa = 8.25TFKK7 pKa = 10.27IKK9 pKa = 10.55RR10 pKa = 11.84ILGKK14 pKa = 9.62KK15 pKa = 8.73QKK17 pKa = 9.07QNRR20 pKa = 11.84PLPQWFRR27 pKa = 11.84FKK29 pKa = 10.23TGNTIRR35 pKa = 11.84YY36 pKa = 5.99NAKK39 pKa = 8.74RR40 pKa = 11.84RR41 pKa = 11.84NWRR44 pKa = 11.84RR45 pKa = 11.84TKK47 pKa = 10.67RR48 pKa = 11.84KK49 pKa = 8.91FF50 pKa = 3.09

Molecular weight: 6.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14606

0

14606

5645737

49

6623

386.5

44.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.855 ± 0.025

1.658 ± 0.017

6.033 ± 0.024

7.149 ± 0.025

4.098 ± 0.019

4.511 ± 0.03

1.696 ± 0.01

8.38 ± 0.032

8.732 ± 0.027

7.753 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.0 ± 0.012

10.029 ± 0.054

3.57 ± 0.021

3.192 ± 0.018

3.107 ± 0.017

8.292 ± 0.03

5.682 ± 0.03

4.852 ± 0.019

0.774 ± 0.007

4.638 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski