Amino acid dipepetide frequency for Emiliania huxleyi (Pontosphaera huxleyi)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	29.764AlaAla: 29.764 ± 0.158	2.61AlaCys: 2.61 ± 0.015	6.533AlaAsp: 6.533 ± 0.024	9.633AlaGlu: 9.633 ± 0.071	3.817AlaPhe: 3.817 ± 0.023	11.371AlaGly: 11.371 ± 0.044	2.699AlaHis: 2.699 ± 0.016	3.533AlaIle: 3.533 ± 0.019	3.838AlaLys: 3.838 ± 0.03	14.17AlaLeu: 14.17 ± 0.052	2.513AlaMet: 2.513 ± 0.015	2.299AlaAsn: 2.299 ± 0.014	8.474AlaPro: 8.474 ± 0.036	3.983AlaGln: 3.983 ± 0.021	10.471AlaArg: 10.471 ± 0.045	10.674AlaSer: 10.674 ± 0.042	6.88AlaThr: 6.88 ± 0.029	9.557AlaVal: 9.557 ± 0.037	1.824AlaTrp: 1.824 ± 0.015	2.071AlaTyr: 2.071 ± 0.016	0.0AlaXaa: 0.0 ± 0.0
Cys	2.495CysAla: 2.495 ± 0.017	0.652CysCys: 0.652 ± 0.01	1.123CysAsp: 1.123 ± 0.01	1.114CysGlu: 1.114 ± 0.011	0.654CysPhe: 0.654 ± 0.009	2.091CysGly: 2.091 ± 0.016	0.431CysHis: 0.431 ± 0.006	0.542CysIle: 0.542 ± 0.007	0.547CysLys: 0.547 ± 0.007	1.664CysLeu: 1.664 ± 0.014	0.324CysMet: 0.324 ± 0.005	0.444CysAsn: 0.444 ± 0.006	1.072CysPro: 1.072 ± 0.013	0.43CysGln: 0.43 ± 0.007	1.441CysArg: 1.441 ± 0.013	1.684CysSer: 1.684 ± 0.017	1.007CysThr: 1.007 ± 0.011	1.408CysVal: 1.408 ± 0.013	0.325CysTrp: 0.325 ± 0.006	0.41CysTyr: 0.41 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	7.332AspAla: 7.332 ± 0.028	0.863AspCys: 0.863 ± 0.01	2.956AspAsp: 2.956 ± 0.019	3.796AspGlu: 3.796 ± 0.023	1.536AspPhe: 1.536 ± 0.013	5.648AspGly: 5.648 ± 0.038	0.828AspHis: 0.828 ± 0.008	1.265AspIle: 1.265 ± 0.012	1.387AspLys: 1.387 ± 0.013	4.07AspLeu: 4.07 ± 0.02	0.822AspMet: 0.822 ± 0.01	0.877AspAsn: 0.877 ± 0.008	2.736AspPro: 2.736 ± 0.017	1.051AspGln: 1.051 ± 0.01	2.703AspArg: 2.703 ± 0.017	2.915AspSer: 2.915 ± 0.017	2.003AspThr: 2.003 ± 0.014	3.294AspVal: 3.294 ± 0.019	0.757AspTrp: 0.757 ± 0.009	0.988AspTyr: 0.988 ± 0.01	0.0AspXaa: 0.0 ± 0.0
Glu	10.655GluAla: 10.655 ± 0.076	0.984GluCys: 0.984 ± 0.011	2.574GluAsp: 2.574 ± 0.017	5.473GluGlu: 5.473 ± 0.044	1.251GluPhe: 1.251 ± 0.01	5.403GluGly: 5.403 ± 0.047	1.3GluHis: 1.3 ± 0.01	1.451GluIle: 1.451 ± 0.012	1.78GluLys: 1.78 ± 0.018	5.953GluLeu: 5.953 ± 0.03	1.232GluMet: 1.232 ± 0.012	0.88GluAsn: 0.88 ± 0.009	3.32GluPro: 3.32 ± 0.02	2.278GluGln: 2.278 ± 0.016	5.422GluArg: 5.422 ± 0.029	3.18GluSer: 3.18 ± 0.019	2.402GluThr: 2.402 ± 0.018	3.892GluVal: 3.892 ± 0.021	1.038GluTrp: 1.038 ± 0.01	1.156GluTyr: 1.156 ± 0.01	0.0GluXaa: 0.0 ± 0.0
Phe	3.671PheAla: 3.671 ± 0.019	0.676PheCys: 0.676 ± 0.008	1.944PheAsp: 1.944 ± 0.015	1.853PheGlu: 1.853 ± 0.012	1.166PhePhe: 1.166 ± 0.013	2.855PheGly: 2.855 ± 0.017	0.616PheHis: 0.616 ± 0.007	0.802PheIle: 0.802 ± 0.008	0.815PheLys: 0.815 ± 0.009	2.677PheLeu: 2.677 ± 0.019	0.518PheMet: 0.518 ± 0.007	0.727PheAsn: 0.727 ± 0.008	1.355PhePro: 1.355 ± 0.011	0.686PheGln: 0.686 ± 0.009	1.693PheArg: 1.693 ± 0.011	2.197PheSer: 2.197 ± 0.015	1.408PheThr: 1.408 ± 0.012	2.283PheVal: 2.283 ± 0.015	0.465PheTrp: 0.465 ± 0.007	0.752PheTyr: 0.752 ± 0.009	0.0PheXaa: 0.0 ± 0.0
Gly	11.717GlyAla: 11.717 ± 0.047	1.944GlyCys: 1.944 ± 0.018	4.516GlyAsp: 4.516 ± 0.03	6.022GlyGlu: 6.022 ± 0.053	2.57GlyPhe: 2.57 ± 0.016	13.634GlyGly: 13.634 ± 0.15	1.725GlyHis: 1.725 ± 0.014	2.169GlyIle: 2.169 ± 0.015	2.693GlyLys: 2.693 ± 0.02	7.058GlyLeu: 7.058 ± 0.032	1.648GlyMet: 1.648 ± 0.014	1.543GlyAsn: 1.543 ± 0.014	3.725GlyPro: 3.725 ± 0.018	2.196GlyGln: 2.196 ± 0.016	6.531GlyArg: 6.531 ± 0.031	6.496GlySer: 6.496 ± 0.03	3.948GlyThr: 3.948 ± 0.023	5.956GlyVal: 5.956 ± 0.03	1.578GlyTrp: 1.578 ± 0.013	1.83GlyTyr: 1.83 ± 0.016	0.0GlyXaa: 0.0 ± 0.0
His	3.112HisAla: 3.112 ± 0.02	0.497HisCys: 0.497 ± 0.007	0.983HisAsp: 0.983 ± 0.011	1.248HisGlu: 1.248 ± 0.01	0.787HisPhe: 0.787 ± 0.009	2.111HisGly: 2.111 ± 0.016	0.618HisHis: 0.618 ± 0.008	0.63HisIle: 0.63 ± 0.008	0.568HisLys: 0.568 ± 0.007	2.145HisLeu: 2.145 ± 0.014	0.388HisMet: 0.388 ± 0.006	0.457HisAsn: 0.457 ± 0.006	1.414HisPro: 1.414 ± 0.012	0.579HisGln: 0.579 ± 0.007	1.656HisArg: 1.656 ± 0.012	1.49HisSer: 1.49 ± 0.011	1.122HisThr: 1.122 ± 0.011	1.542HisVal: 1.542 ± 0.012	0.342HisTrp: 0.342 ± 0.005	0.499HisTyr: 0.499 ± 0.006	0.0HisXaa: 0.0 ± 0.0
Ile	3.512IleAla: 3.512 ± 0.019	0.547IleCys: 0.547 ± 0.008	1.707IleAsp: 1.707 ± 0.011	1.699IleGlu: 1.699 ± 0.013	0.996IlePhe: 0.996 ± 0.011	2.389IleGly: 2.389 ± 0.018	0.53IleHis: 0.53 ± 0.008	0.81IleIle: 0.81 ± 0.009	0.886IleLys: 0.886 ± 0.012	2.144IleLeu: 2.144 ± 0.016	0.476IleMet: 0.476 ± 0.007	0.726IleAsn: 0.726 ± 0.008	1.177IlePro: 1.177 ± 0.013	0.654IleGln: 0.654 ± 0.008	1.525IleArg: 1.525 ± 0.012	1.928IleSer: 1.928 ± 0.015	1.316IleThr: 1.316 ± 0.01	1.922IleVal: 1.922 ± 0.013	0.329IleTrp: 0.329 ± 0.006	0.575IleTyr: 0.575 ± 0.006	0.0IleXaa: 0.0 ± 0.0
Lys	3.831LysAla: 3.831 ± 0.03	0.441LysCys: 0.441 ± 0.007	1.18LysAsp: 1.18 ± 0.012	1.852LysGlu: 1.852 ± 0.018	0.689LysPhe: 0.689 ± 0.007	2.227LysGly: 2.227 ± 0.018	0.629LysHis: 0.629 ± 0.008	0.844LysIle: 0.844 ± 0.009	1.557LysLys: 1.557 ± 0.018	2.91LysLeu: 2.91 ± 0.018	0.64LysMet: 0.64 ± 0.008	0.632LysAsn: 0.632 ± 0.009	1.684LysPro: 1.684 ± 0.013	1.12LysGln: 1.12 ± 0.012	2.415LysArg: 2.415 ± 0.017	1.592LysSer: 1.592 ± 0.015	1.34LysThr: 1.34 ± 0.013	1.773LysVal: 1.773 ± 0.014	0.408LysTrp: 0.408 ± 0.006	0.633LysTyr: 0.633 ± 0.008	0.0LysXaa: 0.0 ± 0.0
Leu	13.337LeuAla: 13.337 ± 0.044	2.141LeuCys: 2.141 ± 0.014	4.776LeuAsp: 4.776 ± 0.024	5.517LeuGlu: 5.517 ± 0.028	2.972LeuPhe: 2.972 ± 0.017	7.625LeuGly: 7.625 ± 0.027	2.603LeuHis: 2.603 ± 0.016	2.332LeuIle: 2.332 ± 0.016	2.665LeuLys: 2.665 ± 0.018	12.276LeuLeu: 12.276 ± 0.049	1.58LeuMet: 1.58 ± 0.012	1.823LeuAsn: 1.823 ± 0.013	6.914LeuPro: 6.914 ± 0.028	3.625LeuGln: 3.625 ± 0.021	8.507LeuArg: 8.507 ± 0.036	7.607LeuSer: 7.607 ± 0.032	4.513LeuThr: 4.513 ± 0.024	6.608LeuVal: 6.608 ± 0.03	1.39LeuTrp: 1.39 ± 0.01	1.885LeuTyr: 1.885 ± 0.014	0.0LeuXaa: 0.0 ± 0.0
Met	2.3MetAla: 2.3 ± 0.014	0.323MetCys: 0.323 ± 0.005	0.756MetAsp: 0.756 ± 0.008	0.93MetGlu: 0.93 ± 0.01	0.453MetPhe: 0.453 ± 0.008	1.294MetGly: 1.294 ± 0.012	0.474MetHis: 0.474 ± 0.007	0.488MetIle: 0.488 ± 0.006	0.507MetLys: 0.507 ± 0.007	2.175MetLeu: 2.175 ± 0.014	0.441MetMet: 0.441 ± 0.006	0.359MetAsn: 0.359 ± 0.006	1.309MetPro: 1.309 ± 0.013	0.762MetGln: 0.762 ± 0.01	1.609MetArg: 1.609 ± 0.014	1.264MetSer: 1.264 ± 0.01	0.866MetThr: 0.866 ± 0.009	1.109MetVal: 1.109 ± 0.01	0.248MetTrp: 0.248 ± 0.004	0.363MetTyr: 0.363 ± 0.006	0.0MetXaa: 0.0 ± 0.0
Asn	2.345AsnAla: 2.345 ± 0.015	0.383AsnCys: 0.383 ± 0.006	0.912AsnAsp: 0.912 ± 0.01	1.078AsnGlu: 1.078 ± 0.01	0.677AsnPhe: 0.677 ± 0.008	1.87AsnGly: 1.87 ± 0.015	0.44AsnHis: 0.44 ± 0.006	0.665AsnIle: 0.665 ± 0.009	0.678AsnLys: 0.678 ± 0.009	1.827AsnLeu: 1.827 ± 0.015	0.381AsnMet: 0.381 ± 0.006	0.536AsnAsn: 0.536 ± 0.009	1.136AsnPro: 1.136 ± 0.014	0.541AsnGln: 0.541 ± 0.008	1.1AsnArg: 1.1 ± 0.01	1.2AsnSer: 1.2 ± 0.01	0.917AsnThr: 0.917 ± 0.009	1.39AsnVal: 1.39 ± 0.011	0.292AsnTrp: 0.292 ± 0.005	0.482AsnTyr: 0.482 ± 0.007	0.0AsnXaa: 0.0 ± 0.0
Pro	8.899ProAla: 8.899 ± 0.043	1.027ProCys: 1.027 ± 0.009	2.804ProAsp: 2.804 ± 0.018	3.187ProGlu: 3.187 ± 0.022	1.741ProPhe: 1.741 ± 0.013	4.24ProGly: 4.24 ± 0.02	1.484ProHis: 1.484 ± 0.013	1.398ProIle: 1.398 ± 0.012	1.499ProLys: 1.499 ± 0.012	6.417ProLeu: 6.417 ± 0.029	0.96ProMet: 0.96 ± 0.009	1.147ProAsn: 1.147 ± 0.014	8.836ProPro: 8.836 ± 0.093	1.896ProGln: 1.896 ± 0.017	4.721ProArg: 4.721 ± 0.028	5.564ProSer: 5.564 ± 0.03	3.144ProThr: 3.144 ± 0.018	3.338ProVal: 3.338 ± 0.017	0.853ProTrp: 0.853 ± 0.008	0.985ProTyr: 0.985 ± 0.01	0.0ProXaa: 0.0 ± 0.0
Gln	4.003GlnAla: 4.003 ± 0.02	0.541GlnCys: 0.541 ± 0.008	0.964GlnAsp: 0.964 ± 0.009	1.497GlnGlu: 1.497 ± 0.013	0.724GlnPhe: 0.724 ± 0.009	2.006GlnGly: 2.006 ± 0.015	0.873GlnHis: 0.873 ± 0.008	0.851GlnIle: 0.851 ± 0.009	0.877GlnLys: 0.877 ± 0.01	3.827GlnLeu: 3.827 ± 0.022	0.662GlnMet: 0.662 ± 0.009	0.531GlnAsn: 0.531 ± 0.008	2.293GlnPro: 2.293 ± 0.019	1.844GlnGln: 1.844 ± 0.028	3.233GlnArg: 3.233 ± 0.02	1.779GlnSer: 1.779 ± 0.011	1.313GlnThr: 1.313 ± 0.012	1.667GlnVal: 1.667 ± 0.011	0.489GlnTrp: 0.489 ± 0.007	0.618GlnTyr: 0.618 ± 0.009	0.0GlnXaa: 0.0 ± 0.0
Arg	10.298ArgAla: 10.298 ± 0.045	1.624ArgCys: 1.624 ± 0.012	3.304ArgAsp: 3.304 ± 0.018	4.64ArgGlu: 4.64 ± 0.028	2.196ArgPhe: 2.196 ± 0.014	6.42ArgGly: 6.42 ± 0.029	1.982ArgHis: 1.982 ± 0.015	1.901ArgIle: 1.901 ± 0.015	2.225ArgLys: 2.225 ± 0.017	8.452ArgLeu: 8.452 ± 0.035	1.399ArgMet: 1.399 ± 0.012	1.282ArgAsn: 1.282 ± 0.013	4.626ArgPro: 4.626 ± 0.024	2.631ArgGln: 2.631 ± 0.015	8.791ArgArg: 8.791 ± 0.051	5.333ArgSer: 5.333 ± 0.023	3.253ArgThr: 3.253 ± 0.017	4.993ArgVal: 4.993 ± 0.021	1.368ArgTrp: 1.368 ± 0.012	1.344ArgTyr: 1.344 ± 0.011	0.0ArgXaa: 0.0 ± 0.0
Ser	9.555SerAla: 9.555 ± 0.035	1.437SerCys: 1.437 ± 0.013	3.415SerAsp: 3.415 ± 0.018	3.711SerGlu: 3.711 ± 0.018	2.341SerPhe: 2.341 ± 0.015	6.544SerGly: 6.544 ± 0.032	1.535SerHis: 1.535 ± 0.012	2.068SerIle: 2.068 ± 0.017	1.894SerLys: 1.894 ± 0.016	7.634SerLeu: 7.634 ± 0.037	1.234SerMet: 1.234 ± 0.01	1.407SerAsn: 1.407 ± 0.011	5.105SerPro: 5.105 ± 0.033	1.9SerGln: 1.9 ± 0.015	5.273SerArg: 5.273 ± 0.027	6.613SerSer: 6.613 ± 0.039	3.642SerThr: 3.642 ± 0.017	4.378SerVal: 4.378 ± 0.023	1.102SerTrp: 1.102 ± 0.012	1.321SerTyr: 1.321 ± 0.01	0.0SerXaa: 0.0 ± 0.0
Thr	6.624ThrAla: 6.624 ± 0.028	0.951ThrCys: 0.951 ± 0.012	2.108ThrAsp: 2.108 ± 0.016	2.249ThrGlu: 2.249 ± 0.014	1.586ThrPhe: 1.586 ± 0.013	3.717ThrGly: 3.717 ± 0.018	1.03ThrHis: 1.03 ± 0.01	1.494ThrIle: 1.494 ± 0.012	1.27ThrLys: 1.27 ± 0.013	4.93ThrLeu: 4.93 ± 0.024	0.879ThrMet: 0.879 ± 0.008	0.998ThrAsn: 0.998 ± 0.01	3.345ThrPro: 3.345 ± 0.016	1.219ThrGln: 1.219 ± 0.012	3.118ThrArg: 3.118 ± 0.016	3.457ThrSer: 3.457 ± 0.017	2.521ThrThr: 2.521 ± 0.017	3.241ThrVal: 3.241 ± 0.016	0.689ThrTrp: 0.689 ± 0.008	0.957ThrTyr: 0.957 ± 0.011	0.0ThrXaa: 0.0 ± 0.0
Val	9.382ValAla: 9.382 ± 0.037	1.418ValCys: 1.418 ± 0.012	3.409ValAsp: 3.409 ± 0.019	4.11ValGlu: 4.11 ± 0.024	1.884ValPhe: 1.884 ± 0.012	5.416ValGly: 5.416 ± 0.027	1.51ValHis: 1.51 ± 0.011	1.681ValIle: 1.681 ± 0.013	1.709ValLys: 1.709 ± 0.015	6.71ValLeu: 6.71 ± 0.029	1.215ValMet: 1.215 ± 0.012	1.281ValAsn: 1.281 ± 0.011	3.795ValPro: 3.795 ± 0.019	2.056ValGln: 2.056 ± 0.014	4.965ValArg: 4.965 ± 0.022	4.586ValSer: 4.586 ± 0.027	3.048ValThr: 3.048 ± 0.016	5.119ValVal: 5.119 ± 0.024	0.989ValTrp: 0.989 ± 0.009	1.431ValTyr: 1.431 ± 0.012	0.0ValXaa: 0.0 ± 0.0
Trp	1.677TrpAla: 1.677 ± 0.013	0.357TrpCys: 0.357 ± 0.006	0.703TrpAsp: 0.703 ± 0.008	0.812TrpGlu: 0.812 ± 0.009	0.402TrpPhe: 0.402 ± 0.006	1.055TrpGly: 1.055 ± 0.01	0.422TrpHis: 0.422 ± 0.006	0.364TrpIle: 0.364 ± 0.005	0.4TrpLys: 0.4 ± 0.006	1.749TrpLeu: 1.749 ± 0.012	0.329TrpMet: 0.329 ± 0.005	0.356TrpAsn: 0.356 ± 0.006	0.863TrpPro: 0.863 ± 0.009	0.578TrpGln: 0.578 ± 0.007	1.631TrpArg: 1.631 ± 0.014	1.128TrpSer: 1.128 ± 0.01	0.738TrpThr: 0.738 ± 0.008	0.889TrpVal: 0.889 ± 0.009	0.321TrpTrp: 0.321 ± 0.005	0.333TrpTyr: 0.333 ± 0.006	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.207TyrAla: 2.207 ± 0.016	0.44TyrCys: 0.44 ± 0.007	1.161TyrAsp: 1.161 ± 0.012	1.202TyrGlu: 1.202 ± 0.011	0.718TyrPhe: 0.718 ± 0.009	1.584TyrGly: 1.584 ± 0.014	0.461TyrHis: 0.461 ± 0.007	0.56TyrIle: 0.56 ± 0.006	0.603TyrLys: 0.603 ± 0.008	1.907TyrLeu: 1.907 ± 0.014	0.388TyrMet: 0.388 ± 0.006	0.546TyrAsn: 0.546 ± 0.008	0.958TyrPro: 0.958 ± 0.01	0.564TyrGln: 0.564 ± 0.007	1.257TyrArg: 1.257 ± 0.012	1.423TyrSer: 1.423 ± 0.011	1.003TyrThr: 1.003 ± 0.011	1.355TyrVal: 1.355 ± 0.012	0.298TyrTrp: 0.298 ± 0.006	0.567TyrTyr: 0.567 ± 0.008	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 35707 proteins (12569600 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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