Mycobacterium phage Pomar16

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 93 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1C9LYD7|A0A1C9LYD7_9CAUD Phosphoesterase OS=Mycobacterium phage Pomar16 OX=1897543 GN=71 PE=4 SV=1
MM1 pKa = 7.68KK2 pKa = 10.64DD3 pKa = 2.97EE4 pKa = 5.99DD5 pKa = 3.83IEE7 pKa = 4.32KK8 pKa = 10.62LKK10 pKa = 10.85SAYY13 pKa = 9.88EE14 pKa = 4.2GFKK17 pKa = 11.02EE18 pKa = 4.08MLDD21 pKa = 3.49NKK23 pKa = 10.6YY24 pKa = 11.22DD25 pKa = 4.66FINEE29 pKa = 4.04QTNDD33 pKa = 3.79GQDD36 pKa = 3.15EE37 pKa = 4.34PEE39 pKa = 4.58DD40 pKa = 3.43YY41 pKa = 11.03GRR43 pKa = 11.84YY44 pKa = 9.85DD45 pKa = 3.44EE46 pKa = 6.16AVTDD50 pKa = 3.83YY51 pKa = 11.52YY52 pKa = 11.4NYY54 pKa = 9.87EE55 pKa = 3.8LAYY58 pKa = 10.71LGEE61 pKa = 4.4LLADD65 pKa = 4.26AVAEE69 pKa = 3.89ALGIKK74 pKa = 9.76EE75 pKa = 4.29DD76 pKa = 3.8SS77 pKa = 3.52

Molecular weight: 8.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1C9LY84|A0A1C9LY84_9CAUD Lysin B OS=Mycobacterium phage Pomar16 OX=1897543 GN=14 PE=4 SV=1
MM1 pKa = 7.26SWAGSKK7 pKa = 9.81RR8 pKa = 11.84RR9 pKa = 11.84QEE11 pKa = 4.41LPEE14 pKa = 4.36DD15 pKa = 3.29WEE17 pKa = 4.35LNYY20 pKa = 10.51RR21 pKa = 11.84LPVLSAAGWLCEE33 pKa = 3.95VDD35 pKa = 4.35GPGCVRR41 pKa = 11.84AATDD45 pKa = 3.4VDD47 pKa = 3.89HH48 pKa = 7.26KK49 pKa = 11.42KK50 pKa = 10.38PGNDD54 pKa = 3.02HH55 pKa = 6.58SRR57 pKa = 11.84SNLQAICRR65 pKa = 11.84VCHH68 pKa = 5.79GKK70 pKa = 10.33KK71 pKa = 9.87SAAEE75 pKa = 3.95GVARR79 pKa = 11.84RR80 pKa = 11.84RR81 pKa = 11.84EE82 pKa = 3.88LKK84 pKa = 10.3ARR86 pKa = 11.84RR87 pKa = 11.84KK88 pKa = 9.96RR89 pKa = 11.84PEE91 pKa = 3.3QRR93 pKa = 11.84HH94 pKa = 5.2PGRR97 pKa = 11.84RR98 pKa = 3.42

Molecular weight: 11.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

93

0

93

16398

25

837

176.3

19.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.916 ± 0.366

0.909 ± 0.125

6.428 ± 0.221

6.915 ± 0.238

3.531 ± 0.16

8.233 ± 0.327

2.195 ± 0.184

5.031 ± 0.164

4.635 ± 0.26

8.153 ± 0.301

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.36 ± 0.12

3.256 ± 0.143

5.342 ± 0.234

3.561 ± 0.204

6.58 ± 0.337

5.001 ± 0.201

5.781 ± 0.241

7.19 ± 0.189

2.025 ± 0.145

2.958 ± 0.171
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski