Pepper cryptic virus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Duplopiviricetes; Durnavirales; Partitiviridae; Deltapartitivirus

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|F8VBM8|F8VBM8_9VIRU Coat protein OS=Pepper cryptic virus 1 OX=369643 PE=4 SV=1
MM1 pKa = 7.88GDD3 pKa = 3.38RR4 pKa = 11.84VNAQDD9 pKa = 4.59DD10 pKa = 4.3DD11 pKa = 4.54TVVPHH16 pKa = 6.01QAPLQPAALQQDD28 pKa = 4.4LTRR31 pKa = 11.84SADD34 pKa = 3.58YY35 pKa = 11.27LLDD38 pKa = 3.36NVRR41 pKa = 11.84IGNHH45 pKa = 4.19RR46 pKa = 11.84QRR48 pKa = 11.84YY49 pKa = 7.93DD50 pKa = 2.67KK51 pKa = 10.73YY52 pKa = 10.4RR53 pKa = 11.84RR54 pKa = 11.84YY55 pKa = 10.27VLLRR59 pKa = 11.84SSEE62 pKa = 4.01IFTSLVAIYY71 pKa = 10.47AHH73 pKa = 6.84IFSSYY78 pKa = 6.8WQHH81 pKa = 5.67FRR83 pKa = 11.84RR84 pKa = 11.84FTDD87 pKa = 3.17QFQAPTGVQLPTFVARR103 pKa = 11.84VYY105 pKa = 10.69ISTWLHH111 pKa = 6.62DD112 pKa = 4.76LYY114 pKa = 11.32CSIRR118 pKa = 11.84EE119 pKa = 4.26ATRR122 pKa = 11.84SISPLAFNEE131 pKa = 4.36RR132 pKa = 11.84YY133 pKa = 9.91SYY135 pKa = 11.27EE136 pKa = 3.94LLPYY140 pKa = 8.73STEE143 pKa = 3.83YY144 pKa = 11.39DD145 pKa = 3.39PFLAFLSMSIKK156 pKa = 8.74PTHH159 pKa = 6.11IQHH162 pKa = 6.04TPEE165 pKa = 3.65NTLWIPILCEE175 pKa = 3.72NYY177 pKa = 10.18DD178 pKa = 3.24WDD180 pKa = 4.94RR181 pKa = 11.84NEE183 pKa = 5.04ANHH186 pKa = 6.33NPFGITNFTLNSNLFYY202 pKa = 11.28GLLAILKK209 pKa = 8.72EE210 pKa = 3.99RR211 pKa = 11.84KK212 pKa = 8.52EE213 pKa = 4.38FKK215 pKa = 10.78LSTLTTNTIGRR226 pKa = 11.84PCWLFDD232 pKa = 3.0WHH234 pKa = 7.77DD235 pKa = 3.96NVQVCAWFPRR245 pKa = 11.84EE246 pKa = 4.33ANFNSQDD253 pKa = 2.95VTAAYY258 pKa = 9.49IIGVACTPKK267 pKa = 10.53LGPSDD272 pKa = 3.96DD273 pKa = 5.02DD274 pKa = 3.06AWKK277 pKa = 10.77YY278 pKa = 9.26YY279 pKa = 11.16ASLNSVPTFTPTEE292 pKa = 3.72PRR294 pKa = 11.84LTNRR298 pKa = 11.84RR299 pKa = 11.84SYY301 pKa = 10.72GAYY304 pKa = 9.19EE305 pKa = 3.77VRR307 pKa = 11.84TRR309 pKa = 11.84EE310 pKa = 3.9TEE312 pKa = 3.83NNYY315 pKa = 9.68FLPDD319 pKa = 3.29SLLNIIEE326 pKa = 5.08DD327 pKa = 4.21FTATGTTQRR336 pKa = 11.84RR337 pKa = 11.84KK338 pKa = 9.5IRR340 pKa = 11.84RR341 pKa = 11.84PSATSASTGAAIIIRR356 pKa = 11.84DD357 pKa = 3.97TPGTASTATTSTTEE371 pKa = 4.15TEE373 pKa = 4.34VTFPPVIRR381 pKa = 11.84TKK383 pKa = 10.39IRR385 pKa = 11.84DD386 pKa = 3.28WYY388 pKa = 7.78YY389 pKa = 8.68HH390 pKa = 5.59SRR392 pKa = 11.84VILEE396 pKa = 4.6LEE398 pKa = 4.5DD399 pKa = 3.79NSRR402 pKa = 11.84TAALRR407 pKa = 11.84MFIIAA412 pKa = 4.64

Molecular weight:
47.57 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|F8VBM8|F8VBM8_9VIRU Coat protein OS=Pepper cryptic virus 1 OX=369643 PE=4 SV=1
MM1 pKa = 7.47VRR3 pKa = 11.84GTLVGYY9 pKa = 10.32DD10 pKa = 3.38YY11 pKa = 11.01TQFQGDD17 pKa = 4.27LVKK20 pKa = 10.09STHH23 pKa = 5.37RR24 pKa = 11.84HH25 pKa = 3.93PHH27 pKa = 4.3VVHH30 pKa = 6.91RR31 pKa = 11.84EE32 pKa = 3.47IATTYY37 pKa = 8.82VDD39 pKa = 3.4QYY41 pKa = 11.03AYY43 pKa = 10.35EE44 pKa = 4.71HH45 pKa = 6.97IEE47 pKa = 4.42TFSSLYY53 pKa = 10.1PEE55 pKa = 6.06LILKK59 pKa = 8.85GWSRR63 pKa = 11.84SYY65 pKa = 10.97YY66 pKa = 10.59LPEE69 pKa = 3.73KK70 pKa = 10.48HH71 pKa = 6.31LAAVLNYY78 pKa = 10.89SMPNVPASQLSQSLYY93 pKa = 9.15RR94 pKa = 11.84QAIEE98 pKa = 3.84SAKK101 pKa = 10.78NGFISLPRR109 pKa = 11.84VKK111 pKa = 10.6AFDD114 pKa = 3.61VLTEE118 pKa = 3.89MDD120 pKa = 3.63QVPFKK125 pKa = 10.65SSSSAGYY132 pKa = 10.35NYY134 pKa = 9.84TGRR137 pKa = 11.84KK138 pKa = 8.81GLIGDD143 pKa = 4.25EE144 pKa = 4.11NHH146 pKa = 6.1SRR148 pKa = 11.84AISIAKK154 pKa = 9.31AVLWSAIKK162 pKa = 10.42DD163 pKa = 3.46DD164 pKa = 4.31GEE166 pKa = 4.7GIEE169 pKa = 4.28HH170 pKa = 7.42VIRR173 pKa = 11.84TSVPDD178 pKa = 3.2VGYY181 pKa = 8.74TRR183 pKa = 11.84TQLTDD188 pKa = 3.73LLEE191 pKa = 4.36KK192 pKa = 10.3TKK194 pKa = 10.27VRR196 pKa = 11.84QVWGRR201 pKa = 11.84AFHH204 pKa = 6.55YY205 pKa = 10.25ILLEE209 pKa = 3.96GLVAYY214 pKa = 8.33PFIQTVMSHH223 pKa = 5.15KK224 pKa = 9.44TFIHH228 pKa = 6.77AGQDD232 pKa = 3.46PLISVPRR239 pKa = 11.84LLSDD243 pKa = 3.79VALNCKK249 pKa = 9.3WIYY252 pKa = 10.66SLDD255 pKa = 3.26WSQFDD260 pKa = 3.45ATVSRR265 pKa = 11.84FEE267 pKa = 3.43IHH269 pKa = 6.71AAFDD273 pKa = 3.98IIKK276 pKa = 10.37SYY278 pKa = 11.8VDD280 pKa = 3.67FPNYY284 pKa = 7.73EE285 pKa = 3.86TEE287 pKa = 3.97QAFEE291 pKa = 3.83ITRR294 pKa = 11.84QLFIHH299 pKa = 6.53KK300 pKa = 9.66KK301 pKa = 8.28VAVPDD306 pKa = 4.33GYY308 pKa = 10.21IYY310 pKa = 10.12EE311 pKa = 4.24SHH313 pKa = 6.77KK314 pKa = 10.8GIPSGSYY321 pKa = 7.44YY322 pKa = 10.2TSLVGSIINYY332 pKa = 9.38LRR334 pKa = 11.84INYY337 pKa = 8.45LWRR340 pKa = 11.84LLTGHH345 pKa = 7.76PPQQCHH351 pKa = 5.2TLGDD355 pKa = 3.97DD356 pKa = 4.19SLVGDD361 pKa = 4.06NSYY364 pKa = 11.54VNPQAIEE371 pKa = 3.66EE372 pKa = 4.17AANKK376 pKa = 9.98LGWHH380 pKa = 6.56FNPDD384 pKa = 2.74KK385 pKa = 9.97TQYY388 pKa = 9.19STVPEE393 pKa = 4.7EE394 pKa = 3.75ITFLGRR400 pKa = 11.84TYY402 pKa = 11.16VGGLNKK408 pKa = 9.58RR409 pKa = 11.84DD410 pKa = 3.34LTKK413 pKa = 10.69CIRR416 pKa = 11.84LLVYY420 pKa = 9.84PEE422 pKa = 4.25YY423 pKa = 9.79PVEE426 pKa = 4.16SGRR429 pKa = 11.84ISAYY433 pKa = 8.84RR434 pKa = 11.84AKK436 pKa = 10.64SIAQDD441 pKa = 3.16AGGLSEE447 pKa = 4.34VLNRR451 pKa = 11.84IADD454 pKa = 3.76KK455 pKa = 10.81LRR457 pKa = 11.84RR458 pKa = 11.84IYY460 pKa = 9.59GTASEE465 pKa = 4.82EE466 pKa = 3.81EE467 pKa = 3.97VPIYY471 pKa = 9.99FKK473 pKa = 10.73RR474 pKa = 11.84YY475 pKa = 8.22VFGVV479 pKa = 3.32

Molecular weight:
54.46 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

891

412

479

445.5

51.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.071 ± 0.441

0.898 ± 0.2

5.387 ± 0.277

5.275 ± 0.266

4.377 ± 0.456

4.826 ± 1.212

3.03 ± 0.382

6.846 ± 0.185

3.704 ± 0.962

8.642 ± 0.093

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

0.786 ± 0.036

4.153 ± 0.752

5.163 ± 0.42

3.704 ± 0.194

6.622 ± 0.879

7.632 ± 0.376

7.856 ± 1.634

6.061 ± 1.071

1.796 ± 0.246

6.173 ± 0.528

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski