Bacillus phage BC01

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Bastillevirinae; Tsarbombavirus; unclassified Tsarbombavirus

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 239 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A346TNK4|A0A346TNK4_9CAUD Uncharacterized protein OS=Bacillus phage BC01 OX=2039884 GN=BC01_047 PE=4 SV=1
MM1 pKa = 7.64KK2 pKa = 10.34LYY4 pKa = 8.47QTSYY8 pKa = 11.32NPTASFKK15 pKa = 11.13KK16 pKa = 10.13EE17 pKa = 3.67MSNVEE22 pKa = 4.4LYY24 pKa = 11.15GIDD27 pKa = 4.15AIIDD31 pKa = 3.54EE32 pKa = 5.09MYY34 pKa = 10.76HH35 pKa = 7.68DD36 pKa = 5.88DD37 pKa = 4.89ALLTNAEE44 pKa = 4.35GLGLAYY50 pKa = 10.42VSSGDD55 pKa = 3.39YY56 pKa = 11.46AMYY59 pKa = 10.33NICNGDD65 pKa = 3.6YY66 pKa = 10.02EE67 pKa = 4.7HH68 pKa = 7.14EE69 pKa = 4.37GVYY72 pKa = 10.5LSYY75 pKa = 10.55FAITEE80 pKa = 4.09DD81 pKa = 3.31GRR83 pKa = 11.84LWAVAYY89 pKa = 10.17DD90 pKa = 3.9DD91 pKa = 4.61EE92 pKa = 4.73EE93 pKa = 4.88EE94 pKa = 4.14EE95 pKa = 4.52VFFVVEE101 pKa = 4.01NN102 pKa = 3.83

Molecular weight: 11.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A346TNM0|A0A346TNM0_9CAUD Uncharacterized protein OS=Bacillus phage BC01 OX=2039884 GN=BC01_063 PE=4 SV=1
MM1 pKa = 7.14SRR3 pKa = 11.84RR4 pKa = 11.84VRR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84AVSRR12 pKa = 11.84CKK14 pKa = 10.63GSNSEE19 pKa = 4.25TKK21 pKa = 9.12TYY23 pKa = 8.39WRR25 pKa = 11.84NRR27 pKa = 11.84VRR29 pKa = 11.84DD30 pKa = 3.46KK31 pKa = 10.71ATNLTPEE38 pKa = 3.96YY39 pKa = 9.06MLSYY43 pKa = 10.3RR44 pKa = 11.84NRR46 pKa = 11.84RR47 pKa = 11.84RR48 pKa = 11.84QLFNEE53 pKa = 5.05LYY55 pKa = 10.52HH56 pKa = 7.34NYY58 pKa = 8.51TQVLNKK64 pKa = 10.09ARR66 pKa = 11.84TEE68 pKa = 4.0GMTIDD73 pKa = 4.59LNGTVTGRR81 pKa = 11.84FKK83 pKa = 11.3SNEE86 pKa = 3.79PNIAGTPKK94 pKa = 10.03TIGGKK99 pKa = 8.06DD100 pKa = 3.17HH101 pKa = 6.82EE102 pKa = 4.6

Molecular weight: 11.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

239

0

239

50697

74

1300

212.1

24.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.407 ± 0.189

1.095 ± 0.084

5.513 ± 0.131

7.014 ± 0.23

4.18 ± 0.11

6.269 ± 0.209

1.87 ± 0.1

6.521 ± 0.13

7.669 ± 0.198

8.35 ± 0.195

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.748 ± 0.093

5.359 ± 0.153

3.562 ± 0.151

3.584 ± 0.121

4.641 ± 0.122

6.48 ± 0.225

6.411 ± 0.198

6.898 ± 0.142

1.158 ± 0.066

4.272 ± 0.118
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski