Saccharothrix sp. CB00851

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Saccharothrix; unclassified Saccharothrix

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7942 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Q4Y218|A0A1Q4Y218_9PSEU Uncharacterized protein OS=Saccharothrix sp. CB00851 OX=1835005 GN=A6A25_30955 PE=3 SV=1
MM1 pKa = 8.25DD2 pKa = 5.58PFCLFMVLPLVVIAGILIWGGLAWVGIVVIFCAAIVVVIDD42 pKa = 3.09SWTNRR47 pKa = 11.84PLPFEE52 pKa = 4.82LPEE55 pKa = 5.98DD56 pKa = 4.66DD57 pKa = 5.06YY58 pKa = 11.76DD59 pKa = 4.27YY60 pKa = 11.4DD61 pKa = 3.87PPQTGRR67 pKa = 11.84RR68 pKa = 11.84AYY70 pKa = 10.31

Molecular weight: 7.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Q4X7P5|A0A1Q4X7P5_9PSEU Uncharacterized protein OS=Saccharothrix sp. CB00851 OX=1835005 GN=A6A25_11825 PE=4 SV=1
MM1 pKa = 7.53SKK3 pKa = 10.53GKK5 pKa = 8.66RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84AKK17 pKa = 8.7THH19 pKa = 5.15GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AILAARR37 pKa = 11.84RR38 pKa = 11.84GKK40 pKa = 10.25GRR42 pKa = 11.84KK43 pKa = 8.28QLSAA47 pKa = 3.9

Molecular weight: 5.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7942

0

7942

2649883

33

6284

333.7

35.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.141 ± 0.045

0.742 ± 0.007

6.321 ± 0.023

5.505 ± 0.024

2.865 ± 0.017

9.085 ± 0.028

2.342 ± 0.013

2.945 ± 0.014

1.923 ± 0.02

10.48 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.564 ± 0.01

1.9 ± 0.018

5.951 ± 0.02

2.634 ± 0.017

8.171 ± 0.03

4.918 ± 0.017

6.178 ± 0.028

9.766 ± 0.031

1.598 ± 0.011

1.971 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski