Butterbur mosaic virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Betaflexiviridae; Quinvirinae; Carlavirus

Average proteome isoelectric point is 7.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D2Z044|D2Z044_9VIRU Movement protein TGB2 OS=Butterbur mosaic virus OX=666859 PE=3 SV=1
MM1 pKa = 7.85DD2 pKa = 3.88VLVAKK7 pKa = 10.23CLEE10 pKa = 4.09FGFIRR15 pKa = 11.84VSISSSKK22 pKa = 9.79PQVIHH27 pKa = 6.22CVPGAGKK34 pKa = 10.33SSLIRR39 pKa = 11.84AILRR43 pKa = 11.84ADD45 pKa = 3.15SRR47 pKa = 11.84FYY49 pKa = 11.29AVTGGVPDD57 pKa = 4.63PVTGQQGRR65 pKa = 11.84ILPLDD70 pKa = 3.84GTAHH74 pKa = 7.17PGACFKK80 pKa = 11.07LVDD83 pKa = 4.31EE84 pKa = 4.36YY85 pKa = 11.4TEE87 pKa = 3.96AVEE90 pKa = 4.76AIEE93 pKa = 4.12GAFAIFGDD101 pKa = 4.14PVQSKK106 pKa = 9.78RR107 pKa = 11.84ASPLLPNFISLNTRR121 pKa = 11.84RR122 pKa = 11.84FGSSTCDD129 pKa = 3.41LLKK132 pKa = 10.91VFGFEE137 pKa = 4.2VYY139 pKa = 8.42STKK142 pKa = 10.89EE143 pKa = 3.81DD144 pKa = 3.45VVQIARR150 pKa = 11.84ADD152 pKa = 3.54QSEE155 pKa = 4.61VEE157 pKa = 4.33GKK159 pKa = 10.75LIVLGDD165 pKa = 3.63EE166 pKa = 4.61AKK168 pKa = 10.9ALACYY173 pKa = 10.47YY174 pKa = 10.39NLEE177 pKa = 4.21YY178 pKa = 10.16LTADD182 pKa = 3.4SARR185 pKa = 11.84GKK187 pKa = 8.55TYY189 pKa = 10.44PVVTLLTGFSEE200 pKa = 5.11VPAEE204 pKa = 4.47EE205 pKa = 4.58YY206 pKa = 10.1PDD208 pKa = 5.31LYY210 pKa = 11.47VCLTRR215 pKa = 11.84HH216 pKa = 5.12QEE218 pKa = 3.92KK219 pKa = 10.8LLVLTGDD226 pKa = 4.4ASCTPAA232 pKa = 5.2

Molecular weight: 25.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D2Z047|D2Z047_9VIRU RNA silencing suppressor OS=Butterbur mosaic virus OX=666859 PE=3 SV=1
MM1 pKa = 7.68SKK3 pKa = 10.5RR4 pKa = 11.84FVKK7 pKa = 10.44RR8 pKa = 11.84FLQARR13 pKa = 11.84FRR15 pKa = 11.84DD16 pKa = 4.05VSGLPKK22 pKa = 10.69GSFGDD27 pKa = 4.31LINIIVSRR35 pKa = 11.84VFEE38 pKa = 4.31GEE40 pKa = 3.99PGQSKK45 pKa = 9.16YY46 pKa = 11.14AKK48 pKa = 9.06EE49 pKa = 3.67RR50 pKa = 11.84RR51 pKa = 11.84AMKK54 pKa = 10.06IGRR57 pKa = 11.84CPRR60 pKa = 11.84CYY62 pKa = 9.39RR63 pKa = 11.84VSPGFYY69 pKa = 9.14FTTRR73 pKa = 11.84CDD75 pKa = 3.36GVTCVPGISYY85 pKa = 10.68NVAKK89 pKa = 9.19MQYY92 pKa = 9.42IKK94 pKa = 11.2YY95 pKa = 10.1GVV97 pKa = 3.03

Molecular weight: 11.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2824

70

1997

470.7

52.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.905 ± 0.682

2.514 ± 0.553

5.276 ± 0.241

5.984 ± 0.916

5.701 ± 0.413

6.763 ± 0.658

2.797 ± 0.511

4.958 ± 0.292

6.268 ± 0.546

10.375 ± 1.266

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.983 ± 0.377

4.214 ± 0.772

4.71 ± 0.885

2.797 ± 0.261

5.028 ± 0.444

8.357 ± 0.32

5.524 ± 0.388

5.595 ± 1.079

0.744 ± 0.214

3.506 ± 0.621
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski