Halovirus HCTV-5

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified archaeal dsDNA viruses; Haloviruses

Average proteome isoelectric point is 4.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 166 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R4TLQ7|R4TLQ7_9VIRU Uncharacterized protein OS=Halovirus HCTV-5 OX=1273748 GN=37 PE=4 SV=1
MM1 pKa = 7.99LYY3 pKa = 10.62RR4 pKa = 11.84MDD6 pKa = 3.67EE7 pKa = 4.3HH8 pKa = 6.34LTSAVSRR15 pKa = 11.84AFEE18 pKa = 4.23KK19 pKa = 11.29EE20 pKa = 3.18EE21 pKa = 4.29DD22 pKa = 4.04LPDD25 pKa = 4.04GAVDD29 pKa = 3.97EE30 pKa = 5.67DD31 pKa = 4.19DD32 pKa = 4.59TEE34 pKa = 4.29ASPMDD39 pKa = 3.64IEE41 pKa = 4.99HH42 pKa = 5.81EE43 pKa = 4.09QIYY46 pKa = 10.68YY47 pKa = 10.91LLADD51 pKa = 5.14AYY53 pKa = 9.65PDD55 pKa = 3.58QFDD58 pKa = 4.01GPEE61 pKa = 3.81DD62 pKa = 3.64ARR64 pKa = 11.84DD65 pKa = 3.82QIHH68 pKa = 6.26EE69 pKa = 4.01ALGVEE74 pKa = 4.36EE75 pKa = 4.58EE76 pKa = 4.54KK77 pKa = 11.21GVDD80 pKa = 3.6DD81 pKa = 4.78EE82 pKa = 4.6EE83 pKa = 5.18DD84 pKa = 3.76RR85 pKa = 11.84EE86 pKa = 4.3EE87 pKa = 4.09TSEE90 pKa = 4.86DD91 pKa = 3.56FNPANTPDD99 pKa = 4.33NLDD102 pKa = 3.19QHH104 pKa = 6.91PYY106 pKa = 11.1DD107 pKa = 3.63EE108 pKa = 5.84AEE110 pKa = 3.82WQEE113 pKa = 4.4DD114 pKa = 4.23YY115 pKa = 10.99EE116 pKa = 4.88AEE118 pKa = 4.24YY119 pKa = 11.36GDD121 pKa = 4.14DD122 pKa = 3.53QAEE125 pKa = 4.34EE126 pKa = 4.34KK127 pKa = 11.06ACDD130 pKa = 3.66HH131 pKa = 6.54TDD133 pKa = 2.82HH134 pKa = 7.4RR135 pKa = 11.84GRR137 pKa = 11.84PLDD140 pKa = 3.8EE141 pKa = 6.22DD142 pKa = 3.59EE143 pKa = 5.63CPWCGEE149 pKa = 4.07DD150 pKa = 5.98DD151 pKa = 3.95EE152 pKa = 5.24

Molecular weight: 17.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R4T7K3|R4T7K3_9VIRU Uncharacterized protein OS=Halovirus HCTV-5 OX=1273748 GN=145 PE=4 SV=1
MM1 pKa = 7.46ASEE4 pKa = 4.38LSGMVKK10 pKa = 8.46TWQVVAAGRR19 pKa = 11.84LSWNGPYY26 pKa = 10.73DD27 pKa = 4.08RR28 pKa = 11.84MPHH31 pKa = 6.38GGTNPYY37 pKa = 9.01RR38 pKa = 11.84WRR40 pKa = 11.84RR41 pKa = 11.84SSISQHH47 pKa = 5.72SLGEE51 pKa = 4.03GQAQVSSS58 pKa = 3.77

Molecular weight: 6.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

166

0

166

32513

33

1293

195.9

22.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.705 ± 0.212

1.261 ± 0.099

9.707 ± 0.282

10.522 ± 0.314

3.122 ± 0.112

7.459 ± 0.219

2.365 ± 0.129

3.946 ± 0.132

4.008 ± 0.166

6.723 ± 0.16

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.436 ± 0.097

3.758 ± 0.136

3.909 ± 0.138

3.559 ± 0.111

6.087 ± 0.202

6.124 ± 0.193

5.773 ± 0.203

7.317 ± 0.162

1.762 ± 0.095

3.457 ± 0.124
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski