Prevotella sp. oral taxon 317 str. F0108

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Prevotellaceae; Prevotella; unclassified Prevotella; Prevotella sp. oral taxon 317

Average proteome isoelectric point is 6.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2926 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D3IH30|D3IH30_9BACT Uncharacterized protein OS=Prevotella sp. oral taxon 317 str. F0108 OX=575615 GN=HMPREF0670_00649 PE=4 SV=1
MM1 pKa = 7.6KK2 pKa = 9.97EE3 pKa = 4.26KK4 pKa = 9.91LTEE7 pKa = 4.1GCIMLVFIAFVVLGSTMAVNMFQQGAWVKK36 pKa = 10.75GIICVLGALFFGFPLLGPFLPSAAPDD62 pKa = 3.69TKK64 pKa = 10.36PLPFVPQVNLPTDD77 pKa = 3.74KK78 pKa = 11.27DD79 pKa = 3.9SLRR82 pKa = 11.84EE83 pKa = 3.9LAKK86 pKa = 10.15MLAGEE91 pKa = 4.53EE92 pKa = 4.11ADD94 pKa = 3.53VMQTVEE100 pKa = 4.68EE101 pKa = 4.2LLEE104 pKa = 4.42SPEE107 pKa = 4.15AFYY110 pKa = 10.92SAQITRR116 pKa = 11.84DD117 pKa = 2.57GWYY120 pKa = 9.59NDD122 pKa = 3.65AYY124 pKa = 11.12VDD126 pKa = 3.36IWDD129 pKa = 4.71MYY131 pKa = 10.83HH132 pKa = 6.92DD133 pKa = 4.3QPDD136 pKa = 3.97VLCSEE141 pKa = 4.29GLLFVLAEE149 pKa = 4.21AEE151 pKa = 4.37VIAMFDD157 pKa = 3.04WKK159 pKa = 10.74EE160 pKa = 3.78GLEE163 pKa = 4.11EE164 pKa = 4.67FVGQMTDD171 pKa = 3.51LRR173 pKa = 11.84RR174 pKa = 11.84AQANNLPVPQEE185 pKa = 4.28HH186 pKa = 7.0FDD188 pKa = 3.68EE189 pKa = 4.73QADD192 pKa = 3.73IPHH195 pKa = 7.08WCNALNEE202 pKa = 4.19LWQPLGYY209 pKa = 10.22NATFIDD215 pKa = 3.93TDD217 pKa = 3.42GDD219 pKa = 3.88EE220 pKa = 4.71YY221 pKa = 10.89IVAVVQYY228 pKa = 9.05TPSPPIDD235 pKa = 5.49DD236 pKa = 4.31ISCTTQSS243 pKa = 3.02

Molecular weight: 27.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D3IIL1|D3IIL1_9BACT Pyridoxal 5'-phosphate synthase subunit PdxT OS=Prevotella sp. oral taxon 317 str. F0108 OX=575615 GN=pdxT PE=3 SV=1
MM1 pKa = 7.54HH2 pKa = 7.37KK3 pKa = 11.04GEE5 pKa = 4.54FFISKK10 pKa = 9.67QSNYY14 pKa = 10.0DD15 pKa = 3.35DD16 pKa = 4.78KK17 pKa = 11.36RR18 pKa = 11.84ITGQTCLADD27 pKa = 3.94DD28 pKa = 3.77RR29 pKa = 11.84RR30 pKa = 11.84GIAFPCTTRR39 pKa = 11.84QYY41 pKa = 8.73GHH43 pKa = 6.8KK44 pKa = 8.79WRR46 pKa = 11.84NSKK49 pKa = 10.56GINRR53 pKa = 11.84QRR55 pKa = 11.84KK56 pKa = 7.52KK57 pKa = 10.65RR58 pKa = 11.84YY59 pKa = 8.28VSGLAGIARR68 pKa = 11.84LFRR71 pKa = 11.84CSLPTANRR79 pKa = 11.84IKK81 pKa = 10.63QGGKK85 pKa = 8.64INRR88 pKa = 11.84AITQVGCKK96 pKa = 9.66IIVEE100 pKa = 4.22AEE102 pKa = 4.02LALEE106 pKa = 3.89LAGRR110 pKa = 11.84KK111 pKa = 7.58TGGRR115 pKa = 3.58

Molecular weight: 12.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2926

0

2926

1017484

30

2564

347.7

39.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.779 ± 0.041

1.212 ± 0.018

5.559 ± 0.029

5.892 ± 0.046

4.483 ± 0.026

6.873 ± 0.042

2.034 ± 0.021

5.818 ± 0.035

6.493 ± 0.039

9.123 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.751 ± 0.022

5.335 ± 0.039

3.796 ± 0.023

3.774 ± 0.029

5.072 ± 0.031

5.762 ± 0.04

5.893 ± 0.039

6.778 ± 0.032

1.274 ± 0.017

4.299 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski