Listeria phage P35

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A8ATT6|A8ATT6_9CAUD Gp48 OS=Listeria phage P35 OX=330398 PE=4 SV=1
MM1 pKa = 7.92CEE3 pKa = 4.12YY4 pKa = 10.98CNDD7 pKa = 3.84DD8 pKa = 3.61YY9 pKa = 11.3TVRR12 pKa = 11.84SHH14 pKa = 7.39LEE16 pKa = 3.78TPEE19 pKa = 3.78GHH21 pKa = 6.75EE22 pKa = 4.51IEE24 pKa = 4.2IDD26 pKa = 3.52KK27 pKa = 11.3EE28 pKa = 3.98NDD30 pKa = 3.02LSITLDD36 pKa = 3.63LDD38 pKa = 3.17ISEE41 pKa = 4.26DD42 pKa = 4.33FIWIEE47 pKa = 3.71IKK49 pKa = 10.48YY50 pKa = 9.97CPWCGRR56 pKa = 11.84KK57 pKa = 9.72LSDD60 pKa = 3.66

Molecular weight: 7.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A8ATR1|A8ATR1_9CAUD Gp23 OS=Listeria phage P35 OX=330398 PE=4 SV=1
MM1 pKa = 7.53TKK3 pKa = 9.96TNYY6 pKa = 9.87VLDD9 pKa = 3.52QLTPEE14 pKa = 4.04EE15 pKa = 4.18RR16 pKa = 11.84QLVEE20 pKa = 4.17QSTQLKK26 pKa = 7.99STTVAFWLNLLGSFACDD43 pKa = 4.5FYY45 pKa = 11.0TGRR48 pKa = 11.84ILAGFIKK55 pKa = 10.54VLLSIASSGIGAVIFTIWGLFTYY78 pKa = 10.25RR79 pKa = 11.84SRR81 pKa = 11.84VRR83 pKa = 11.84KK84 pKa = 9.93YY85 pKa = 10.94NNQLLTDD92 pKa = 3.76KK93 pKa = 11.35AFMVNLGKK101 pKa = 8.65PTSKK105 pKa = 10.83GDD107 pKa = 3.47EE108 pKa = 4.18KK109 pKa = 11.54

Molecular weight: 12.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

10977

34

635

196.0

22.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.575 ± 0.333

0.729 ± 0.112

7.288 ± 0.272

6.678 ± 0.422

4.455 ± 0.226

6.158 ± 0.451

1.831 ± 0.199

6.933 ± 0.259

7.762 ± 0.408

7.762 ± 0.273

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.824 ± 0.165

5.584 ± 0.277

3.434 ± 0.234

3.835 ± 0.219

4.382 ± 0.282

5.484 ± 0.2

6.714 ± 0.373

7.015 ± 0.224

1.248 ± 0.144

4.309 ± 0.253
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski