Paracoccus phage vB_PthS_Pthi1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3Q9R8Y3|A0A3Q9R8Y3_9CAUD Uncharacterized protein OS=Paracoccus phage vB_PthS_Pthi1 OX=2500570 GN=pthi1_p05 PE=4 SV=1
MM1 pKa = 7.09KK2 pKa = 9.87TIEE5 pKa = 4.24QLTASEE11 pKa = 4.3TAWYY15 pKa = 10.1SSNDD19 pKa = 3.04EE20 pKa = 4.43DD21 pKa = 4.08VWTGGPYY28 pKa = 8.79DD29 pKa = 3.71TRR31 pKa = 11.84QEE33 pKa = 4.06AEE35 pKa = 4.3EE36 pKa = 3.77EE37 pKa = 4.37AKK39 pKa = 11.06ANWHH43 pKa = 5.63RR44 pKa = 11.84LIMRR48 pKa = 11.84ATKK51 pKa = 9.99TPIRR55 pKa = 11.84VSKK58 pKa = 10.84HH59 pKa = 4.5FDD61 pKa = 2.81QGGFFEE67 pKa = 4.7AAEE70 pKa = 4.01EE71 pKa = 4.26SLYY74 pKa = 10.98DD75 pKa = 3.95LCNEE79 pKa = 4.96DD80 pKa = 5.19GDD82 pKa = 4.24PTLDD86 pKa = 3.74FDD88 pKa = 4.83PAVNLDD94 pKa = 3.17LQTRR98 pKa = 11.84VRR100 pKa = 11.84AAIDD104 pKa = 3.26EE105 pKa = 4.4WQVAHH110 pKa = 6.38QLAPTPWRR118 pKa = 11.84FSGGDD123 pKa = 3.52EE124 pKa = 4.22PEE126 pKa = 3.46IAAWAKK132 pKa = 10.85AEE134 pKa = 4.07EE135 pKa = 4.89GGDD138 pKa = 3.44AA139 pKa = 4.76

Molecular weight: 15.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3T0IJX0|A0A3T0IJX0_9CAUD Uncharacterized protein OS=Paracoccus phage vB_PthS_Pthi1 OX=2500570 GN=pthi1_p09 PE=4 SV=1
MM1 pKa = 7.33NLARR5 pKa = 11.84LCAQYY10 pKa = 10.42IAAGLDD16 pKa = 3.35PARR19 pKa = 11.84FWEE22 pKa = 4.45VTPRR26 pKa = 11.84LYY28 pKa = 10.85SVEE31 pKa = 3.88IEE33 pKa = 4.25GAGIRR38 pKa = 11.84LEE40 pKa = 4.27RR41 pKa = 11.84EE42 pKa = 3.74RR43 pKa = 11.84EE44 pKa = 4.24MVWWGAMLPHH54 pKa = 7.09LKK56 pKa = 10.05KK57 pKa = 10.93APDD60 pKa = 3.2LKK62 pKa = 10.29TFVGHH67 pKa = 6.91RR68 pKa = 11.84DD69 pKa = 3.33VARR72 pKa = 11.84TRR74 pKa = 11.84AEE76 pKa = 3.81RR77 pKa = 11.84VRR79 pKa = 11.84QFHH82 pKa = 6.23AAWDD86 pKa = 3.94RR87 pKa = 11.84VDD89 pKa = 3.61RR90 pKa = 11.84ALARR94 pKa = 11.84ASRR97 pKa = 3.69

Molecular weight: 11.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

12451

32

1469

239.4

26.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.919 ± 0.544

0.972 ± 0.127

6.409 ± 0.22

5.799 ± 0.347

3.1 ± 0.201

9.084 ± 0.343

1.815 ± 0.133

5.028 ± 0.178

3.807 ± 0.363

8.385 ± 0.27

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.835 ± 0.227

2.787 ± 0.258

5.212 ± 0.232

3.879 ± 0.234

7.943 ± 0.35

5.502 ± 0.34

5.1 ± 0.243

6.417 ± 0.284

1.968 ± 0.122

2.04 ± 0.185
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski