Geodermatophilus sp. Leaf369

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Geodermatophilales; Geodermatophilaceae; Geodermatophilus; unclassified Geodermatophilus

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4093 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q5VF77|A0A0Q5VF77_9ACTN Diacylglycerol kinase OS=Geodermatophilus sp. Leaf369 OX=1736354 GN=ASG36_00055 PE=4 SV=1
MM1 pKa = 7.4AVAGGLVLLAGCTGGSSSSGQADD24 pKa = 3.9GSSAPTSEE32 pKa = 4.88APAAQLAVSPTDD44 pKa = 3.28GTADD48 pKa = 3.49VSPTTPVQLAVTDD61 pKa = 4.2GAIGEE66 pKa = 4.46VTVTDD71 pKa = 3.45AAGTVVPGAVAEE83 pKa = 4.55APADD87 pKa = 3.55PAAPTTAAPGATTSVWTPTDD107 pKa = 3.37PLAYY111 pKa = 8.12GTTYY115 pKa = 10.48TVDD118 pKa = 3.24ATATNADD125 pKa = 4.06DD126 pKa = 5.1AEE128 pKa = 4.55TDD130 pKa = 3.06ASTTFSTVTPASVTTPSIGPLDD152 pKa = 3.94GTTVGVGMPIRR163 pKa = 11.84VFFDD167 pKa = 3.43QPVTDD172 pKa = 3.76QAAVEE177 pKa = 4.19SHH179 pKa = 6.75LLVTSSTPTDD189 pKa = 3.87GRR191 pKa = 11.84WNWVSSSEE199 pKa = 4.03VHH201 pKa = 6.17FRR203 pKa = 11.84PSTYY207 pKa = 9.38WPANTTVTLDD217 pKa = 3.22ADD219 pKa = 4.4LYY221 pKa = 11.56GVDD224 pKa = 4.09TGDD227 pKa = 4.72GIWGEE232 pKa = 4.09KK233 pKa = 10.15DD234 pKa = 2.83RR235 pKa = 11.84TVTFTVGEE243 pKa = 4.19KK244 pKa = 10.09HH245 pKa = 6.7VSVADD250 pKa = 4.05AGSHH254 pKa = 5.1TLTVYY259 pKa = 10.97DD260 pKa = 3.94SDD262 pKa = 4.34QVVQTYY268 pKa = 9.98PMSAGSSEE276 pKa = 3.74NPTRR280 pKa = 11.84NGAHH284 pKa = 5.48VVLEE288 pKa = 4.25KK289 pKa = 11.45YY290 pKa = 10.56EE291 pKa = 4.97DD292 pKa = 3.26ITMDD296 pKa = 2.88SSTFGLAVDD305 pKa = 3.73AAGGYY310 pKa = 7.88RR311 pKa = 11.84TDD313 pKa = 2.79VQYY316 pKa = 10.3ATRR319 pKa = 11.84ISNNGEE325 pKa = 3.95FVHH328 pKa = 6.5AAPWSVGQQGSSNVSHH344 pKa = 6.66GCINLSTDD352 pKa = 3.25RR353 pKa = 11.84AQWFYY358 pKa = 11.71DD359 pKa = 4.28FSQPGDD365 pKa = 3.43VVEE368 pKa = 4.59VVNSIGPTLSPVDD381 pKa = 3.41GDD383 pKa = 3.57IYY385 pKa = 11.3DD386 pKa = 3.74WAIPWDD392 pKa = 3.49QWVAGSALSS401 pKa = 3.48

Molecular weight: 41.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q5VAK0|A0A0Q5VAK0_9ACTN Uncharacterized protein OS=Geodermatophilus sp. Leaf369 OX=1736354 GN=ASG36_09915 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84SKK15 pKa = 8.58THH17 pKa = 5.46GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILAGRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 10.08GRR40 pKa = 11.84EE41 pKa = 3.61KK42 pKa = 10.93LSAA45 pKa = 3.78

Molecular weight: 5.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4093

0

4093

1301898

37

3380

318.1

33.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.309 ± 0.061

0.642 ± 0.01

6.452 ± 0.031

5.263 ± 0.038

2.539 ± 0.023

9.726 ± 0.036

2.034 ± 0.019

2.538 ± 0.026

1.364 ± 0.026

10.719 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.527 ± 0.014

1.376 ± 0.018

6.033 ± 0.038

2.803 ± 0.02

7.577 ± 0.04

4.963 ± 0.026

6.593 ± 0.04

10.425 ± 0.037

1.468 ± 0.015

1.648 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski