Amino acid dipepetide frequency for Paramicrosporidium saccamoebae

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.323AlaAla: 7.323 ± 0.091	1.122AlaCys: 1.122 ± 0.027	3.916AlaAsp: 3.916 ± 0.056	5.002AlaGlu: 5.002 ± 0.066	2.895AlaPhe: 2.895 ± 0.048	4.525AlaGly: 4.525 ± 0.062	1.706AlaHis: 1.706 ± 0.034	4.58AlaIle: 4.58 ± 0.054	4.245AlaLys: 4.245 ± 0.062	8.283AlaLeu: 8.283 ± 0.08	2.095AlaMet: 2.095 ± 0.038	2.639AlaAsn: 2.639 ± 0.043	3.596AlaPro: 3.596 ± 0.054	2.778AlaGln: 2.778 ± 0.046	4.186AlaArg: 4.186 ± 0.053	5.97AlaSer: 5.97 ± 0.072	4.736AlaThr: 4.736 ± 0.063	5.718AlaVal: 5.718 ± 0.061	0.836AlaTrp: 0.836 ± 0.022	1.899AlaTyr: 1.899 ± 0.042	0.002AlaXaa: 0.002 ± 0.001
Cys	1.137CysAla: 1.137 ± 0.027	0.321CysCys: 0.321 ± 0.015	0.795CysAsp: 0.795 ± 0.022	0.855CysGlu: 0.855 ± 0.023	0.638CysPhe: 0.638 ± 0.021	1.045CysGly: 1.045 ± 0.025	0.44CysHis: 0.44 ± 0.017	0.93CysIle: 0.93 ± 0.027	0.79CysLys: 0.79 ± 0.025	1.633CysLeu: 1.633 ± 0.035	0.36CysMet: 0.36 ± 0.014	0.593CysAsn: 0.593 ± 0.021	0.876CysPro: 0.876 ± 0.026	0.657CysGln: 0.657 ± 0.02	1.183CysArg: 1.183 ± 0.031	1.228CysSer: 1.228 ± 0.027	0.902CysThr: 0.902 ± 0.023	0.968CysVal: 0.968 ± 0.027	0.208CysTrp: 0.208 ± 0.01	0.47CysTyr: 0.47 ± 0.018	0.0CysXaa: 0.0 ± 0.0
Asp	4.303AspAla: 4.303 ± 0.054	0.935AspCys: 0.935 ± 0.029	3.214AspAsp: 3.214 ± 0.056	4.139AspGlu: 4.139 ± 0.06	2.33AspPhe: 2.33 ± 0.036	3.43AspGly: 3.43 ± 0.048	1.207AspHis: 1.207 ± 0.028	2.952AspIle: 2.952 ± 0.036	2.313AspLys: 2.313 ± 0.041	5.437AspLeu: 5.437 ± 0.058	1.311AspMet: 1.311 ± 0.032	1.854AspAsn: 1.854 ± 0.034	2.919AspPro: 2.919 ± 0.044	1.78AspGln: 1.78 ± 0.04	3.064AspArg: 3.064 ± 0.048	4.219AspSer: 4.219 ± 0.063	2.702AspThr: 2.702 ± 0.047	3.427AspVal: 3.427 ± 0.051	0.831AspTrp: 0.831 ± 0.022	1.728AspTyr: 1.728 ± 0.035	0.001AspXaa: 0.001 ± 0.001
Glu	5.048GluAla: 5.048 ± 0.063	1.024GluCys: 1.024 ± 0.029	3.686GluAsp: 3.686 ± 0.059	5.628GluGlu: 5.628 ± 0.089	2.431GluPhe: 2.431 ± 0.039	3.363GluGly: 3.363 ± 0.046	1.527GluHis: 1.527 ± 0.028	3.384GluIle: 3.384 ± 0.046	3.756GluLys: 3.756 ± 0.057	6.684GluLeu: 6.684 ± 0.09	2.04GluMet: 2.04 ± 0.037	2.341GluAsn: 2.341 ± 0.042	2.434GluPro: 2.434 ± 0.042	2.596GluGln: 2.596 ± 0.043	4.295GluArg: 4.295 ± 0.059	4.761GluSer: 4.761 ± 0.065	3.804GluThr: 3.804 ± 0.06	3.468GluVal: 3.468 ± 0.049	0.814GluTrp: 0.814 ± 0.024	2.004GluTyr: 2.004 ± 0.039	0.001GluXaa: 0.001 ± 0.001
Phe	3.0PheAla: 3.0 ± 0.049	0.715PheCys: 0.715 ± 0.02	2.447PheAsp: 2.447 ± 0.039	2.42PheGlu: 2.42 ± 0.041	1.592PhePhe: 1.592 ± 0.037	2.809PheGly: 2.809 ± 0.057	0.969PheHis: 0.969 ± 0.026	2.011PheIle: 2.011 ± 0.039	1.799PheLys: 1.799 ± 0.033	3.939PheLeu: 3.939 ± 0.053	0.908PheMet: 0.908 ± 0.027	1.397PheAsn: 1.397 ± 0.029	1.833PhePro: 1.833 ± 0.034	1.391PheGln: 1.391 ± 0.032	2.236PheArg: 2.236 ± 0.035	3.353PheSer: 3.353 ± 0.06	2.075PheThr: 2.075 ± 0.034	2.935PheVal: 2.935 ± 0.046	0.586PheTrp: 0.586 ± 0.023	1.239PheTyr: 1.239 ± 0.031	0.0PheXaa: 0.0 ± 0.0
Gly	4.043GlyAla: 4.043 ± 0.058	0.96GlyCys: 0.96 ± 0.024	3.012GlyAsp: 3.012 ± 0.05	3.451GlyGlu: 3.451 ± 0.051	2.338GlyPhe: 2.338 ± 0.041	3.877GlyGly: 3.877 ± 0.086	1.591GlyHis: 1.591 ± 0.036	3.279GlyIle: 3.279 ± 0.047	3.411GlyLys: 3.411 ± 0.048	5.493GlyLeu: 5.493 ± 0.063	1.639GlyMet: 1.639 ± 0.039	2.162GlyAsn: 2.162 ± 0.04	2.388GlyPro: 2.388 ± 0.04	2.218GlyGln: 2.218 ± 0.042	3.849GlyArg: 3.849 ± 0.057	4.531GlySer: 4.531 ± 0.06	3.417GlyThr: 3.417 ± 0.045	3.654GlyVal: 3.654 ± 0.052	0.826GlyTrp: 0.826 ± 0.023	1.709GlyTyr: 1.709 ± 0.037	0.001GlyXaa: 0.001 ± 0.001
His	1.615HisAla: 1.615 ± 0.042	0.468HisCys: 0.468 ± 0.018	1.141HisAsp: 1.141 ± 0.025	1.257HisGlu: 1.257 ± 0.028	1.111HisPhe: 1.111 ± 0.029	1.547HisGly: 1.547 ± 0.034	0.706HisHis: 0.706 ± 0.023	1.198HisIle: 1.198 ± 0.028	0.972HisLys: 0.972 ± 0.027	2.644HisLeu: 2.644 ± 0.044	0.492HisMet: 0.492 ± 0.017	0.782HisAsn: 0.782 ± 0.021	1.478HisPro: 1.478 ± 0.03	0.917HisGln: 0.917 ± 0.023	1.576HisArg: 1.576 ± 0.036	1.824HisSer: 1.824 ± 0.036	1.057HisThr: 1.057 ± 0.03	1.599HisVal: 1.599 ± 0.03	0.328HisTrp: 0.328 ± 0.014	0.802HisTyr: 0.802 ± 0.021	0.0HisXaa: 0.0 ± 0.0
Ile	4.117IleAla: 4.117 ± 0.047	0.909IleCys: 0.909 ± 0.026	3.077IleAsp: 3.077 ± 0.05	3.455IleGlu: 3.455 ± 0.048	2.223IlePhe: 2.223 ± 0.043	3.009IleGly: 3.009 ± 0.045	1.255IleHis: 1.255 ± 0.025	2.869IleIle: 2.869 ± 0.047	2.804IleLys: 2.804 ± 0.046	5.587IleLeu: 5.587 ± 0.064	1.253IleMet: 1.253 ± 0.029	1.937IleAsn: 1.937 ± 0.034	3.09IlePro: 3.09 ± 0.046	2.013IleGln: 2.013 ± 0.037	3.193IleArg: 3.193 ± 0.047	4.453IleSer: 4.453 ± 0.064	2.795IleThr: 2.795 ± 0.042	3.916IleVal: 3.916 ± 0.057	0.663IleTrp: 0.663 ± 0.022	1.486IleTyr: 1.486 ± 0.029	0.0IleXaa: 0.0 ± 0.0
Lys	3.812LysAla: 3.812 ± 0.063	0.852LysCys: 0.852 ± 0.03	2.719LysAsp: 2.719 ± 0.044	3.497LysGlu: 3.497 ± 0.055	1.821LysPhe: 1.821 ± 0.034	2.525LysGly: 2.525 ± 0.043	1.138LysHis: 1.138 ± 0.031	2.767LysIle: 2.767 ± 0.041	3.409LysLys: 3.409 ± 0.078	5.334LysLeu: 5.334 ± 0.064	1.604LysMet: 1.604 ± 0.035	2.006LysAsn: 2.006 ± 0.036	2.466LysPro: 2.466 ± 0.049	1.875LysGln: 1.875 ± 0.035	3.426LysArg: 3.426 ± 0.054	4.042LysSer: 4.042 ± 0.064	3.369LysThr: 3.369 ± 0.06	3.317LysVal: 3.317 ± 0.052	0.654LysTrp: 0.654 ± 0.022	1.582LysTyr: 1.582 ± 0.034	0.0LysXaa: 0.0 ± 0.0
Leu	8.69LeuAla: 8.69 ± 0.087	1.773LeuCys: 1.773 ± 0.04	5.823LeuAsp: 5.823 ± 0.066	6.915LeuGlu: 6.915 ± 0.069	4.137LeuPhe: 4.137 ± 0.057	5.953LeuGly: 5.953 ± 0.073	2.438LeuHis: 2.438 ± 0.038	4.816LeuIle: 4.816 ± 0.066	5.335LeuLys: 5.335 ± 0.055	11.331LeuLeu: 11.331 ± 0.121	2.256LeuMet: 2.256 ± 0.041	3.591LeuAsn: 3.591 ± 0.049	5.178LeuPro: 5.178 ± 0.064	4.572LeuGln: 4.572 ± 0.057	6.3LeuArg: 6.3 ± 0.068	8.074LeuSer: 8.074 ± 0.078	5.339LeuThr: 5.339 ± 0.054	7.099LeuVal: 7.099 ± 0.067	1.184LeuTrp: 1.184 ± 0.031	2.744LeuTyr: 2.744 ± 0.039	0.001LeuXaa: 0.001 ± 0.001
Met	2.349MetAla: 2.349 ± 0.042	0.299MetCys: 0.299 ± 0.013	1.534MetAsp: 1.534 ± 0.029	1.762MetGlu: 1.762 ± 0.034	0.82MetPhe: 0.82 ± 0.02	1.483MetGly: 1.483 ± 0.038	0.559MetHis: 0.559 ± 0.018	1.263MetIle: 1.263 ± 0.028	1.227MetLys: 1.227 ± 0.028	2.547MetLeu: 2.547 ± 0.041	0.702MetMet: 0.702 ± 0.023	0.944MetAsn: 0.944 ± 0.026	1.201MetPro: 1.201 ± 0.028	1.001MetGln: 1.001 ± 0.023	1.508MetArg: 1.508 ± 0.031	1.967MetSer: 1.967 ± 0.037	1.628MetThr: 1.628 ± 0.031	1.691MetVal: 1.691 ± 0.029	0.242MetTrp: 0.242 ± 0.012	0.587MetTyr: 0.587 ± 0.02	0.0MetXaa: 0.0 ± 0.0
Asn	2.778AsnAla: 2.778 ± 0.045	0.645AsnCys: 0.645 ± 0.022	1.921AsnAsp: 1.921 ± 0.04	2.207AsnGlu: 2.207 ± 0.039	1.439AsnPhe: 1.439 ± 0.031	2.332AsnGly: 2.332 ± 0.044	0.732AsnHis: 0.732 ± 0.021	2.156AsnIle: 2.156 ± 0.039	1.621AsnLys: 1.621 ± 0.034	3.708AsnLeu: 3.708 ± 0.053	0.941AsnMet: 0.941 ± 0.024	1.334AsnAsn: 1.334 ± 0.031	2.084AsnPro: 2.084 ± 0.038	1.24AsnGln: 1.24 ± 0.026	2.015AsnArg: 2.015 ± 0.037	2.792AsnSer: 2.792 ± 0.046	1.87AsnThr: 1.87 ± 0.036	2.842AsnVal: 2.842 ± 0.045	0.491AsnTrp: 0.491 ± 0.017	1.142AsnTyr: 1.142 ± 0.025	0.001AsnXaa: 0.001 ± 0.001
Pro	3.857ProAla: 3.857 ± 0.054	0.636ProCys: 0.636 ± 0.02	2.615ProAsp: 2.615 ± 0.039	3.416ProGlu: 3.416 ± 0.049	1.975ProPhe: 1.975 ± 0.038	2.638ProGly: 2.638 ± 0.043	1.132ProHis: 1.132 ± 0.028	2.723ProIle: 2.723 ± 0.042	2.698ProLys: 2.698 ± 0.048	4.598ProLeu: 4.598 ± 0.05	1.16ProMet: 1.16 ± 0.026	2.0ProAsn: 2.0 ± 0.04	3.188ProPro: 3.188 ± 0.084	1.74ProGln: 1.74 ± 0.038	2.718ProArg: 2.718 ± 0.045	4.572ProSer: 4.572 ± 0.059	3.556ProThr: 3.556 ± 0.063	3.639ProVal: 3.639 ± 0.051	0.529ProTrp: 0.529 ± 0.019	1.272ProTyr: 1.272 ± 0.035	0.001ProXaa: 0.001 ± 0.001
Gln	2.766GlnAla: 2.766 ± 0.044	0.631GlnCys: 0.631 ± 0.022	1.734GlnAsp: 1.734 ± 0.031	2.327GlnGlu: 2.327 ± 0.04	1.499GlnPhe: 1.499 ± 0.033	1.621GlnGly: 1.621 ± 0.035	0.948GlnHis: 0.948 ± 0.025	2.177GlnIle: 2.177 ± 0.043	2.098GlnLys: 2.098 ± 0.038	4.266GlnLeu: 4.266 ± 0.063	1.092GlnMet: 1.092 ± 0.027	1.449GlnAsn: 1.449 ± 0.036	1.784GlnPro: 1.784 ± 0.049	1.915GlnGln: 1.915 ± 0.059	2.576GlnArg: 2.576 ± 0.043	3.073GlnSer: 3.073 ± 0.049	2.086GlnThr: 2.086 ± 0.035	2.375GlnVal: 2.375 ± 0.041	0.493GlnTrp: 0.493 ± 0.018	1.162GlnTyr: 1.162 ± 0.025	0.0GlnXaa: 0.0 ± 0.0
Arg	4.077ArgAla: 4.077 ± 0.052	0.899ArgCys: 0.899 ± 0.024	3.195ArgAsp: 3.195 ± 0.056	3.662ArgGlu: 3.662 ± 0.049	2.431ArgPhe: 2.431 ± 0.034	3.096ArgGly: 3.096 ± 0.049	1.713ArgHis: 1.713 ± 0.032	3.734ArgIle: 3.734 ± 0.051	3.435ArgLys: 3.435 ± 0.051	6.788ArgLeu: 6.788 ± 0.074	1.558ArgMet: 1.558 ± 0.032	2.36ArgAsn: 2.36 ± 0.038	3.163ArgPro: 3.163 ± 0.057	2.556ArgGln: 2.556 ± 0.044	4.837ArgArg: 4.837 ± 0.069	4.303ArgSer: 4.303 ± 0.061	3.369ArgThr: 3.369 ± 0.049	3.808ArgVal: 3.808 ± 0.049	0.796ArgTrp: 0.796 ± 0.024	1.682ArgTyr: 1.682 ± 0.032	0.001ArgXaa: 0.001 ± 0.001
Ser	5.942SerAla: 5.942 ± 0.065	1.178SerCys: 1.178 ± 0.029	4.065SerAsp: 4.065 ± 0.061	4.617SerGlu: 4.617 ± 0.059	3.218SerPhe: 3.218 ± 0.057	4.76SerGly: 4.76 ± 0.063	1.776SerHis: 1.776 ± 0.038	4.399SerIle: 4.399 ± 0.054	4.036SerLys: 4.036 ± 0.059	8.006SerLeu: 8.006 ± 0.086	1.887SerMet: 1.887 ± 0.04	2.897SerAsn: 2.897 ± 0.049	4.269SerPro: 4.269 ± 0.073	2.895SerGln: 2.895 ± 0.051	4.659SerArg: 4.659 ± 0.06	7.195SerSer: 7.195 ± 0.138	4.883SerThr: 4.883 ± 0.073	5.322SerVal: 5.322 ± 0.065	0.964SerTrp: 0.964 ± 0.023	1.889SerTyr: 1.889 ± 0.038	0.001SerXaa: 0.001 ± 0.001
Thr	4.838ThrAla: 4.838 ± 0.061	0.879ThrCys: 0.879 ± 0.024	2.913ThrAsp: 2.913 ± 0.048	3.539ThrGlu: 3.539 ± 0.06	2.21ThrPhe: 2.21 ± 0.04	3.572ThrGly: 3.572 ± 0.052	1.097ThrHis: 1.097 ± 0.03	3.355ThrIle: 3.355 ± 0.045	2.868ThrLys: 2.868 ± 0.042	6.043ThrLeu: 6.043 ± 0.067	1.456ThrMet: 1.456 ± 0.03	2.016ThrAsn: 2.016 ± 0.036	3.35ThrPro: 3.35 ± 0.074	1.764ThrGln: 1.764 ± 0.035	3.224ThrArg: 3.224 ± 0.047	4.149ThrSer: 4.149 ± 0.065	4.109ThrThr: 4.109 ± 0.141	4.442ThrVal: 4.442 ± 0.06	0.609ThrTrp: 0.609 ± 0.02	1.167ThrTyr: 1.167 ± 0.027	0.0ThrXaa: 0.0 ± 0.0
Val	5.67ValAla: 5.67 ± 0.066	1.013ValCys: 1.013 ± 0.027	3.98ValAsp: 3.98 ± 0.053	4.596ValGlu: 4.596 ± 0.055	2.747ValPhe: 2.747 ± 0.05	3.919ValGly: 3.919 ± 0.048	1.579ValHis: 1.579 ± 0.031	3.312ValIle: 3.312 ± 0.05	3.412ValLys: 3.412 ± 0.061	6.963ValLeu: 6.963 ± 0.072	1.55ValMet: 1.55 ± 0.032	2.323ValAsn: 2.323 ± 0.039	3.586ValPro: 3.586 ± 0.05	2.617ValGln: 2.617 ± 0.034	3.816ValArg: 3.816 ± 0.059	5.086ValSer: 5.086 ± 0.066	3.726ValThr: 3.726 ± 0.051	4.707ValVal: 4.707 ± 0.055	0.793ValTrp: 0.793 ± 0.021	1.789ValTyr: 1.789 ± 0.031	0.0ValXaa: 0.0 ± 0.0
Trp	0.757TrpAla: 0.757 ± 0.022	0.198TrpCys: 0.198 ± 0.012	0.746TrpAsp: 0.746 ± 0.023	0.682TrpGlu: 0.682 ± 0.018	0.507TrpPhe: 0.507 ± 0.02	0.552TrpGly: 0.552 ± 0.024	0.295TrpHis: 0.295 ± 0.014	0.791TrpIle: 0.791 ± 0.022	0.781TrpLys: 0.781 ± 0.024	1.325TrpLeu: 1.325 ± 0.032	0.368TrpMet: 0.368 ± 0.015	0.63TrpAsn: 0.63 ± 0.023	0.474TrpPro: 0.474 ± 0.016	0.477TrpGln: 0.477 ± 0.017	0.874TrpArg: 0.874 ± 0.025	1.002TrpSer: 1.002 ± 0.025	0.795TrpThr: 0.795 ± 0.022	0.615TrpVal: 0.615 ± 0.02	0.174TrpTrp: 0.174 ± 0.01	0.358TrpTyr: 0.358 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.927TyrAla: 1.927 ± 0.034	0.572TyrCys: 0.572 ± 0.02	1.562TyrAsp: 1.562 ± 0.031	1.661TyrGlu: 1.661 ± 0.033	1.292TyrPhe: 1.292 ± 0.035	1.791TyrGly: 1.791 ± 0.038	0.706TyrHis: 0.706 ± 0.023	1.408TyrIle: 1.408 ± 0.029	1.203TyrLys: 1.203 ± 0.025	2.938TyrLeu: 2.938 ± 0.051	0.642TyrMet: 0.642 ± 0.02	1.033TyrAsn: 1.033 ± 0.028	1.305TyrPro: 1.305 ± 0.031	1.071TyrGln: 1.071 ± 0.028	1.932TyrArg: 1.932 ± 0.032	2.261TyrSer: 2.261 ± 0.047	1.385TyrThr: 1.385 ± 0.03	1.643TyrVal: 1.643 ± 0.031	0.38TyrTrp: 0.38 ± 0.015	1.044TyrTyr: 1.044 ± 0.027	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.001XaaPhe: 0.001 ± 0.001	0.001XaaGly: 0.001 ± 0.001	0.001XaaHis: 0.001 ± 0.001	0.0XaaIle: 0.0 ± 0.0	0.001XaaLys: 0.001 ± 0.001	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.001XaaAsn: 0.001 ± 0.001	0.001XaaPro: 0.001 ± 0.001	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.001XaaSer: 0.001 ± 0.001	0.001XaaThr: 0.001 ± 0.001	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.236XaaXaa: 0.236 ± 0.086

Statistics based on 3754 proteins (1669180 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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