Bacillus sp. FJAT-25509

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; unclassified Bacillus (in: Bacteria)

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4458 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q3U119|A0A0Q3U119_9BACI APH domain-containing protein OS=Bacillus sp. FJAT-25509 OX=1712029 GN=AN960_13100 PE=4 SV=1
MM1 pKa = 7.47YY2 pKa = 8.82EE3 pKa = 3.95QTDD6 pKa = 3.37SDD8 pKa = 3.98EE9 pKa = 4.53TEE11 pKa = 3.86FAYY14 pKa = 9.82TDD16 pKa = 3.34TEE18 pKa = 4.21MPDD21 pKa = 4.91DD22 pKa = 4.63EE23 pKa = 5.4NCSDD27 pKa = 3.53WEE29 pKa = 4.16FDD31 pKa = 3.71YY32 pKa = 11.53EE33 pKa = 4.46DD34 pKa = 5.25NIWICDD40 pKa = 3.87DD41 pKa = 3.79SSSAYY46 pKa = 9.52YY47 pKa = 10.64RR48 pKa = 11.84NFFFLGSFFATKK60 pKa = 10.9NMLYY64 pKa = 10.68SNNDD68 pKa = 2.89FNQYY72 pKa = 9.77KK73 pKa = 10.29SSGIINGTNSSVKK86 pKa = 10.3KK87 pKa = 10.36SGFGSGVSGGFGGG100 pKa = 4.46

Molecular weight: 11.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q3WA60|A0A0Q3WA60_9BACI Chemotaxis protein CheY OS=Bacillus sp. FJAT-25509 OX=1712029 GN=AN960_04180 PE=4 SV=1
MM1 pKa = 7.75NIRR4 pKa = 11.84NVIAVNTRR12 pKa = 11.84NAIVMSTRR20 pKa = 11.84NVIAVNTKK28 pKa = 9.26NAIVMSTRR36 pKa = 11.84NVIATSTRR44 pKa = 11.84NVIAVNTRR52 pKa = 11.84NAIVMNTKK60 pKa = 10.28NVIVMSTRR68 pKa = 11.84NVIAVNTRR76 pKa = 11.84NAIVTNTRR84 pKa = 11.84NVIVMSTRR92 pKa = 11.84NVIAVLRR99 pKa = 4.21

Molecular weight: 10.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4458

0

4458

1318057

27

2835

295.7

33.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.225 ± 0.039

0.761 ± 0.012

4.899 ± 0.031

6.93 ± 0.051

4.865 ± 0.034

6.673 ± 0.042

1.937 ± 0.017

8.66 ± 0.041

7.528 ± 0.034

9.762 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.512 ± 0.018

5.346 ± 0.034

3.429 ± 0.02

3.422 ± 0.021

3.498 ± 0.026

6.467 ± 0.032

5.54 ± 0.035

6.893 ± 0.03

0.957 ± 0.012

3.696 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski