Escovopsis weberi

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Hypocreomycetidae; Hypocreales; Hypocreaceae; Escovopsis

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6869 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M8N3D9|A0A0M8N3D9_9HYPO Uncharacterized protein OS=Escovopsis weberi OX=150374 GN=ESCO_001134 PE=4 SV=1
MM1 pKa = 7.79DD2 pKa = 3.55VCTTSQQNGFNWSGLAPGEE21 pKa = 4.19VGSYY25 pKa = 11.01DD26 pKa = 3.38GFKK29 pKa = 10.77FKK31 pKa = 10.21GWKK34 pKa = 10.42CEE36 pKa = 4.14DD37 pKa = 3.34NQADD41 pKa = 3.85VNINALGLNVNVGLGLGGSGAQTARR66 pKa = 11.84TIVGDD71 pKa = 3.76LTSNTDD77 pKa = 3.51DD78 pKa = 4.25SPSISADD85 pKa = 3.2GPFSIDD91 pKa = 2.96EE92 pKa = 4.35MQVSTEE98 pKa = 3.71FDD100 pKa = 3.13ADD102 pKa = 3.7LEE104 pKa = 4.39FRR106 pKa = 11.84YY107 pKa = 10.67EE108 pKa = 4.05MADD111 pKa = 2.9GSYY114 pKa = 10.34CMHH117 pKa = 6.58VASCSPSGTTVKK129 pKa = 9.5NTGCHH134 pKa = 5.35GSKK137 pKa = 10.53GVTVVYY143 pKa = 9.96PGSSGSNDD151 pKa = 2.95SCRR154 pKa = 11.84TRR156 pKa = 11.84IHH158 pKa = 7.36GITFNCPNGSSPSGGQQPSQEE179 pKa = 4.22PAQEE183 pKa = 4.15PSQAPTQAPTEE194 pKa = 4.08QPTQVSHH201 pKa = 6.62TSAPDD206 pKa = 3.29ASSTDD211 pKa = 3.6SPVSSGGDD219 pKa = 3.31DD220 pKa = 6.19DD221 pKa = 5.21EE222 pKa = 7.24DD223 pKa = 5.31CDD225 pKa = 3.97EE226 pKa = 4.51TTTAPASAPTALHH239 pKa = 6.67PLRR242 pKa = 11.84PFIPHH247 pKa = 7.14PGADD251 pKa = 3.56ASSDD255 pKa = 3.46APAASSAPATSLRR268 pKa = 11.84IPLHH272 pKa = 5.54TGVPVRR278 pKa = 11.84VPTALPGDD286 pKa = 4.22STTAPAEE293 pKa = 4.17PTSVAPAEE301 pKa = 4.28PANTEE306 pKa = 3.93APAEE310 pKa = 4.18PTSEE314 pKa = 4.35APAEE318 pKa = 4.04PTPTEE323 pKa = 4.19DD324 pKa = 3.89VPAQEE329 pKa = 4.82PVSTDD334 pKa = 3.0APALPEE340 pKa = 4.19STDD343 pKa = 3.42APLEE347 pKa = 4.32TVTTTFQSISTIFTTSIHH365 pKa = 6.72TITSCAPDD373 pKa = 3.61VTDD376 pKa = 4.54CPARR380 pKa = 11.84TSPTVVTEE388 pKa = 4.25TVVHH392 pKa = 6.7DD393 pKa = 3.71TTICPVTATLTRR405 pKa = 11.84TRR407 pKa = 11.84AHH409 pKa = 6.55HH410 pKa = 5.94SAPSAHH416 pKa = 5.93TRR418 pKa = 11.84VAPPTDD424 pKa = 3.7AAPTSIPTQDD434 pKa = 3.03TAAPEE439 pKa = 4.35APAPTSVAPGTGAPEE454 pKa = 4.15APAPTSAAPGTDD466 pKa = 3.7APDD469 pKa = 3.69APAPTSAAPGTDD481 pKa = 3.7APDD484 pKa = 3.58APAPTGAAPGTDD496 pKa = 3.88APDD499 pKa = 4.15APDD502 pKa = 3.86APAPTGAAPGTDD514 pKa = 3.56APEE517 pKa = 4.44APAPTSAAPGTDD529 pKa = 3.26APSSPEE535 pKa = 3.98SASPSAPAEE544 pKa = 4.28AEE546 pKa = 4.14PTSAPAEE553 pKa = 4.41TFVTTYY559 pKa = 10.84QSTSTAYY566 pKa = 8.08VTQVHH571 pKa = 7.02TITSCAPDD579 pKa = 4.04VPDD582 pKa = 4.25CPARR586 pKa = 11.84AGPTVVTEE594 pKa = 4.49TVAVSTTICPVTATMTRR611 pKa = 11.84PAATPSAPAAPVGAPAPTGAPQGSDD636 pKa = 3.15APASPVDD643 pKa = 3.93VPAPADD649 pKa = 3.71SPAPAPAPEE658 pKa = 4.93GSPAPAPDD666 pKa = 4.03APAPAPDD673 pKa = 3.8APAPEE678 pKa = 5.34DD679 pKa = 3.66SPAPADD685 pKa = 3.84APASPEE691 pKa = 3.68NSAPAVIYY699 pKa = 10.74SPVPTTMQSAPGPAGPTAFVPQPTNDD725 pKa = 3.44LPGPPVPTGMPVISGAGRR743 pKa = 11.84LGASAGLLLGLMAAVVLLL761 pKa = 4.07

Molecular weight: 74.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M8MZG8|A0A0M8MZG8_9HYPO Uncharacterized protein OS=Escovopsis weberi OX=150374 GN=ESCO_005626 PE=4 SV=1
MM1 pKa = 7.52SFLARR6 pKa = 11.84LARR9 pKa = 11.84PVVALHH15 pKa = 6.26IQPSTRR21 pKa = 11.84TFTTLQPLRR30 pKa = 11.84PTLLATFRR38 pKa = 11.84PALTSFAPATPSTAAPGAGEE58 pKa = 4.02RR59 pKa = 11.84AADD62 pKa = 4.31LVPAAAVSAHH72 pKa = 6.75PALAGATQIRR82 pKa = 11.84FGPRR86 pKa = 11.84NTMQGHH92 pKa = 5.07TRR94 pKa = 11.84LVQKK98 pKa = 10.38RR99 pKa = 11.84RR100 pKa = 11.84HH101 pKa = 4.91GWLKK105 pKa = 10.59RR106 pKa = 11.84MRR108 pKa = 11.84SKK110 pKa = 9.65TGRR113 pKa = 11.84RR114 pKa = 11.84ILEE117 pKa = 3.75RR118 pKa = 11.84RR119 pKa = 11.84KK120 pKa = 10.16LKK122 pKa = 10.35GRR124 pKa = 11.84RR125 pKa = 11.84HH126 pKa = 4.95VAWW129 pKa = 4.03

Molecular weight: 14.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6869

0

6869

3220148

50

4362

468.8

51.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.003 ± 0.032

1.152 ± 0.011

6.128 ± 0.021

6.321 ± 0.031

3.591 ± 0.018

7.325 ± 0.031

2.435 ± 0.012

4.434 ± 0.019

4.614 ± 0.026

8.891 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.248 ± 0.011

3.258 ± 0.016

5.96 ± 0.032

3.965 ± 0.026

6.675 ± 0.027

7.747 ± 0.028

5.301 ± 0.019

6.11 ± 0.024

1.37 ± 0.011

2.464 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski