Microbacterium sp. AR7-10

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Microbacterium; unclassified Microbacterium

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2773 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1J6XDL3|A0A1J6XDL3_9MICO MFS transporter OS=Microbacterium sp. AR7-10 OX=1891970 GN=BFN01_08120 PE=4 SV=1
MM1 pKa = 7.48TLMGCASGNTGGGSAGDD18 pKa = 3.44GDD20 pKa = 4.0GGYY23 pKa = 10.42RR24 pKa = 11.84IALSMSYY31 pKa = 10.28SGNDD35 pKa = 3.3WQGEE39 pKa = 4.24AQSLVYY45 pKa = 10.21AAAEE49 pKa = 3.94SDD51 pKa = 3.6ALKK54 pKa = 11.03DD55 pKa = 3.57KK56 pKa = 10.87VDD58 pKa = 3.69SVEE61 pKa = 4.08TFIAGTDD68 pKa = 3.34AQTQISQIQQMITAQYY84 pKa = 10.52DD85 pKa = 3.53AILIYY90 pKa = 10.03PISPTALNQVIKK102 pKa = 10.15QGCDD106 pKa = 2.48AGIPMFTYY114 pKa = 10.23DD115 pKa = 3.87AVVTEE120 pKa = 4.67PCAKK124 pKa = 10.3NVTFDD129 pKa = 3.09QAEE132 pKa = 4.14FGKK135 pKa = 10.93VGAEE139 pKa = 3.95YY140 pKa = 10.67LSDD143 pKa = 4.31LMGDD147 pKa = 3.74TGNVVLITGVAGTSVDD163 pKa = 3.6EE164 pKa = 4.86DD165 pKa = 3.52RR166 pKa = 11.84TNAARR171 pKa = 11.84AVFEE175 pKa = 3.97EE176 pKa = 4.22RR177 pKa = 11.84GINVLDD183 pKa = 3.75SCAGDD188 pKa = 3.64WAQGPAGEE196 pKa = 4.38CMSRR200 pKa = 11.84FLAAFDD206 pKa = 5.93DD207 pKa = 4.36IDD209 pKa = 4.28GVWAQVGGVAVPAAFEE225 pKa = 3.85AAGRR229 pKa = 11.84PFVPMIAEE237 pKa = 4.15AEE239 pKa = 4.03NLYY242 pKa = 10.32RR243 pKa = 11.84LHH245 pKa = 6.81LQDD248 pKa = 4.64PEE250 pKa = 4.34YY251 pKa = 10.65IEE253 pKa = 5.66NGLQGGSAGSPPWQSAAALHH273 pKa = 5.37MAVRR277 pKa = 11.84AIEE280 pKa = 4.5GEE282 pKa = 4.3EE283 pKa = 4.17FPSTIDD289 pKa = 4.67VGFDD293 pKa = 2.63WATQEE298 pKa = 4.08DD299 pKa = 4.82LKK301 pKa = 10.89MCEE304 pKa = 3.95TGSAEE309 pKa = 4.09EE310 pKa = 5.35LEE312 pKa = 4.75AGCNTFPAGMVSDD325 pKa = 4.84GFMADD330 pKa = 2.47WWHH333 pKa = 6.78PEE335 pKa = 3.16WTAGIDD341 pKa = 3.97LDD343 pKa = 4.17TLLSVDD349 pKa = 3.23VDD351 pKa = 3.66

Molecular weight: 37.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1J6YEH4|A0A1J6YEH4_9MICO SGL domain-containing protein OS=Microbacterium sp. AR7-10 OX=1891970 GN=BFN01_03875 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 10.04KK16 pKa = 8.86HH17 pKa = 4.36GFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILAARR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.52GRR40 pKa = 11.84TEE42 pKa = 4.14LSAA45 pKa = 4.86

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2773

0

2773

892355

38

2033

321.8

34.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.454 ± 0.072

0.493 ± 0.01

6.442 ± 0.04

5.694 ± 0.038

3.107 ± 0.033

8.833 ± 0.041

2.032 ± 0.021

4.785 ± 0.033

2.025 ± 0.034

10.013 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.981 ± 0.019

1.884 ± 0.025

5.248 ± 0.029

2.939 ± 0.025

7.458 ± 0.051

5.534 ± 0.027

5.874 ± 0.033

8.771 ± 0.043

1.496 ± 0.018

1.936 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski