Sulfolobales Mexican fusellovirus 1

Taxonomy: Viruses; Fuselloviridae; unclassified Fuselloviridae

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 24 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M1SVC2|M1SVC2_9VIRU VP2-like protein OS=Sulfolobales Mexican fusellovirus 1 OX=1298531 GN=SMF1_0024 PE=4 SV=1
MM1 pKa = 7.39KK2 pKa = 10.37KK3 pKa = 10.27AVLVLFLLILPVLAFIPVTTNASTSGSPSITISYY37 pKa = 8.64PSTVIAGQKK46 pKa = 9.95VPITFQFNNTISSTYY61 pKa = 10.61LPILGYY67 pKa = 9.87SVSYY71 pKa = 8.16TQSGSSYY78 pKa = 10.74SYY80 pKa = 11.09TDD82 pKa = 3.49GDD84 pKa = 3.53TSGAYY89 pKa = 9.77AVTTLGANGSVIVINYY105 pKa = 7.99VGTYY109 pKa = 9.56KK110 pKa = 10.67VLEE113 pKa = 4.29GTFPGIVLYY122 pKa = 10.87GGGFEE127 pKa = 4.74TVNPSNGIATLDD139 pKa = 3.58TQQPSTATGVLVTFNGYY156 pKa = 7.96FQYY159 pKa = 11.15LSGGSWHH166 pKa = 6.38NASTSLPYY174 pKa = 10.35YY175 pKa = 10.08GSYY178 pKa = 10.11SSGYY182 pKa = 8.15IQPSSAVTWVIVFEE196 pKa = 4.62DD197 pKa = 4.03SNGEE201 pKa = 4.07TLLKK205 pKa = 10.37SISINGVTYY214 pKa = 9.13TINVLAPVPWNFTYY228 pKa = 10.89VGVRR232 pKa = 11.84PDD234 pKa = 3.45NSNDD238 pKa = 3.8GIGVSTFTVVTPSPYY253 pKa = 8.85QTYY256 pKa = 10.8DD257 pKa = 2.87VYY259 pKa = 11.65LNNNLTVTGEE269 pKa = 4.32TNMSGMGSYY278 pKa = 10.53SFTMPSSSVSVTVEE292 pKa = 3.72WVSANLNKK300 pKa = 9.84TVVLTPVTPSISITYY315 pKa = 9.14PSASYY320 pKa = 10.54AGEE323 pKa = 3.78AVKK326 pKa = 9.11ITFQFLLSNGSTTTEE341 pKa = 4.13EE342 pKa = 4.33ANQSYY347 pKa = 11.09DD348 pKa = 3.44VFIGKK353 pKa = 7.33TLNATGVTNSTGFGTFTFTMPSAPTTVTVEE383 pKa = 4.4WISANLNKK391 pKa = 9.81TVTVNLLAPQISINAPSTVYY411 pKa = 10.44AGQSVPVTFQFLILNEE427 pKa = 4.44SPTTTTAIAQANQGYY442 pKa = 9.25KK443 pKa = 10.1FYY445 pKa = 10.95LGSTLFTTGEE455 pKa = 4.38TNSTGYY461 pKa = 8.66GTVTFTMPSSAITVTVTWVSFNLNKK486 pKa = 10.37SVTLSPSSSTTTSSTTTTSSSTTSSTTSSTTTSTTSSSSTTTTSSTTSSSSTTTSSPDD544 pKa = 2.92AFQLVEE550 pKa = 4.24LTGLAPVVAIIFTFVFMGVTYY571 pKa = 10.26KK572 pKa = 10.3IAGRR576 pKa = 11.84IPAIIVAIITTVAFTFLLFLPLPYY600 pKa = 9.84TLAMLFAFIAGLIYY614 pKa = 10.27TRR616 pKa = 11.84LGGGEE621 pKa = 4.07EE622 pKa = 4.28SGDD625 pKa = 3.51

Molecular weight: 65.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M1T2S8|M1T2S8_9VIRU Uncharacterized protein OS=Sulfolobales Mexican fusellovirus 1 OX=1298531 GN=SMF1_0002 PE=4 SV=1
MM1 pKa = 8.2AEE3 pKa = 4.26EE4 pKa = 4.09KK5 pKa = 9.84WIQKK9 pKa = 9.14AVKK12 pKa = 9.87HH13 pKa = 6.03KK14 pKa = 10.74GRR16 pKa = 11.84VHH18 pKa = 7.16RR19 pKa = 11.84YY20 pKa = 8.46LEE22 pKa = 3.84HH23 pKa = 7.34LYY25 pKa = 10.2GHH27 pKa = 7.03KK28 pKa = 10.47AFKK31 pKa = 10.67EE32 pKa = 3.87NGDD35 pKa = 3.57IKK37 pKa = 11.15VKK39 pKa = 9.17YY40 pKa = 9.52LNMAIRR46 pKa = 11.84HH47 pKa = 4.76VKK49 pKa = 7.96RR50 pKa = 11.84AKK52 pKa = 9.85MPEE55 pKa = 3.77HH56 pKa = 6.98EE57 pKa = 4.51KK58 pKa = 10.8RR59 pKa = 11.84SLLSALYY66 pKa = 8.82LAKK69 pKa = 10.48RR70 pKa = 11.84LRR72 pKa = 11.84HH73 pKa = 4.15MHH75 pKa = 5.93KK76 pKa = 9.81HH77 pKa = 5.97RR78 pKa = 11.84KK79 pKa = 7.2HH80 pKa = 5.68HH81 pKa = 5.29HH82 pKa = 5.7HH83 pKa = 6.87KK84 pKa = 10.52KK85 pKa = 10.4

Molecular weight: 10.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

24

0

24

4637

38

625

193.2

21.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.534 ± 0.346

0.518 ± 0.15

3.084 ± 0.446

7.074 ± 1.039

4.831 ± 0.49

6.211 ± 0.454

1.574 ± 0.419

6.189 ± 0.462

6.427 ± 1.059

10.567 ± 0.609

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.264 ± 0.21

4.119 ± 0.408

4.701 ± 0.428

2.458 ± 0.165

4.205 ± 0.816

7.16 ± 0.974

7.699 ± 1.334

8.13 ± 0.378

1.121 ± 0.186

5.133 ± 0.44
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski