Alkalihalobacillus okhensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Alkalihalobacillus

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4332 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B0I8E7|A0A0B0I8E7_9BACI Acyl-CoA dehydrogenase OS=Alkalihalobacillus okhensis OX=333138 GN=LQ50_19155 PE=3 SV=1
MM1 pKa = 7.28KK2 pKa = 10.52QFFYY6 pKa = 11.23YY7 pKa = 10.24IMILSSIIIIFLGYY21 pKa = 9.9LHH23 pKa = 6.88YY24 pKa = 10.3GQQLTTVVSDD34 pKa = 4.08SADD37 pKa = 3.59HH38 pKa = 6.05EE39 pKa = 4.73PEE41 pKa = 4.64LASQDD46 pKa = 3.29IQEE49 pKa = 4.44EE50 pKa = 4.33QTSYY54 pKa = 11.44EE55 pKa = 3.93LGGLLEE61 pKa = 4.83EE62 pKa = 4.39VAKK65 pKa = 10.84QNEE68 pKa = 4.27DD69 pKa = 3.48EE70 pKa = 4.68EE71 pKa = 5.08EE72 pKa = 3.98FLLMVIGSSAVTSVEE87 pKa = 4.31EE88 pKa = 4.28SSSMPQLLLPQFKK101 pKa = 10.63SLLASQDD108 pKa = 3.22VALTVIDD115 pKa = 4.12VNNATSSEE123 pKa = 4.1VLADD127 pKa = 3.59NYY129 pKa = 10.11IDD131 pKa = 6.06KK132 pKa = 10.51IIKK135 pKa = 9.83NNPDD139 pKa = 3.53LLVIEE144 pKa = 4.83PFLLNDD150 pKa = 3.41YY151 pKa = 10.45DD152 pKa = 3.84QLPVEE157 pKa = 5.04DD158 pKa = 4.52SLSNLEE164 pKa = 4.25TVMSEE169 pKa = 5.04LEE171 pKa = 4.13EE172 pKa = 4.1QLSDD176 pKa = 3.53TLIVLSPAHH185 pKa = 6.71PLDD188 pKa = 4.58DD189 pKa = 5.58DD190 pKa = 4.82GYY192 pKa = 10.4LTFTDD197 pKa = 4.24KK198 pKa = 10.83IKK200 pKa = 11.03AFSAKK205 pKa = 9.98HH206 pKa = 5.22EE207 pKa = 4.17YY208 pKa = 10.53LYY210 pKa = 10.97ADD212 pKa = 4.02HH213 pKa = 7.11WEE215 pKa = 4.12AWPTVANEE223 pKa = 4.2LPNEE227 pKa = 3.81QWHH230 pKa = 6.71RR231 pKa = 11.84IWSDD235 pKa = 3.22YY236 pKa = 11.76LMDD239 pKa = 4.31FLVNN243 pKa = 3.91

Molecular weight: 27.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B0IKR2|A0A0B0IKR2_9BACI Uncharacterized protein OS=Alkalihalobacillus okhensis OX=333138 GN=LQ50_04355 PE=4 SV=1
MM1 pKa = 7.71GKK3 pKa = 8.0PTFQPNNRR11 pKa = 11.84KK12 pKa = 9.23RR13 pKa = 11.84KK14 pKa = 8.22KK15 pKa = 8.69VHH17 pKa = 5.46GFRR20 pKa = 11.84ARR22 pKa = 11.84MSTKK26 pKa = 10.19NGRR29 pKa = 11.84KK30 pKa = 8.49VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.05GRR40 pKa = 11.84KK41 pKa = 8.7VLSAA45 pKa = 4.05

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4332

0

4332

1252721

26

1734

289.2

32.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.872 ± 0.043

0.726 ± 0.01

5.064 ± 0.027

7.75 ± 0.043

4.566 ± 0.033

6.864 ± 0.038

2.184 ± 0.021

7.765 ± 0.041

6.344 ± 0.036

9.904 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.829 ± 0.018

4.238 ± 0.026

3.57 ± 0.021

3.858 ± 0.027

4.149 ± 0.026

5.977 ± 0.025

5.464 ± 0.02

7.377 ± 0.034

1.062 ± 0.014

3.438 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski