Pseudomonas phage SM1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Samunavirus; Pseudomonas virus SM1

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 129 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0U2UUX5|A0A0U2UUX5_9CAUD Uncharacterized protein OS=Pseudomonas phage SM1 OX=1772332 GN=SM1_0113 PE=4 SV=1
MM1 pKa = 7.52NYY3 pKa = 10.86ANVSNLLEE11 pKa = 3.73VSYY14 pKa = 10.98NGKK17 pKa = 9.26EE18 pKa = 4.02VYY20 pKa = 10.3HH21 pKa = 6.55PIEE24 pKa = 4.22VNTLIFHH31 pKa = 7.21LMDD34 pKa = 3.75TLGVAVEE41 pKa = 4.36SRR43 pKa = 11.84VTLYY47 pKa = 10.87LDD49 pKa = 3.51KK50 pKa = 11.41ASVGWVFGICVDD62 pKa = 3.83EE63 pKa = 4.18EE64 pKa = 4.94AIFDD68 pKa = 3.76LWRR71 pKa = 11.84YY72 pKa = 10.33SEE74 pKa = 4.68TEE76 pKa = 3.85FPPWLEE82 pKa = 3.89IEE84 pKa = 4.27PLL86 pKa = 3.58

Molecular weight: 9.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0U2UUS4|A0A0U2UUS4_9CAUD Crossover junction endodeoxyribonuclease RuvC OS=Pseudomonas phage SM1 OX=1772332 GN=SM1_049 PE=3 SV=1
MM1 pKa = 7.36ATFNDD6 pKa = 3.77VAAGAALSRR15 pKa = 11.84LSRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84ATEE23 pKa = 3.74SEE25 pKa = 4.2VAMPADD31 pKa = 3.32VCLFAWLTAEE41 pKa = 5.16AARR44 pKa = 11.84QNSNPFTISARR55 pKa = 11.84QIQFGFSDD63 pKa = 3.1EE64 pKa = 4.13RR65 pKa = 11.84GRR67 pKa = 11.84VDD69 pKa = 4.58KK70 pKa = 11.46VGLSVNTIALGLEE83 pKa = 3.82RR84 pKa = 11.84LKK86 pKa = 11.18EE87 pKa = 3.77NGFIQVEE94 pKa = 4.25QVSRR98 pKa = 11.84LRR100 pKa = 11.84GGGRR104 pKa = 11.84LMSITIVGSWHH115 pKa = 5.8GAVEE119 pKa = 4.09RR120 pKa = 11.84RR121 pKa = 11.84KK122 pKa = 10.45KK123 pKa = 10.38SAA125 pKa = 3.09

Molecular weight: 13.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

129

0

129

28475

38

1750

220.7

24.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.658 ± 0.436

1.159 ± 0.1

5.791 ± 0.144

7.09 ± 0.256

3.642 ± 0.141

7.41 ± 0.221

1.868 ± 0.139

4.72 ± 0.14

4.439 ± 0.204

8.931 ± 0.203

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.416 ± 0.121

3.631 ± 0.116

4.548 ± 0.223

3.747 ± 0.27

7.28 ± 0.261

6.43 ± 0.18

5.384 ± 0.205

7.322 ± 0.2

1.352 ± 0.097

3.182 ± 0.164
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski