Clostridiaceae bacterium OM08-6BH

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; unclassified Clostridiaceae

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3400 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A417PSY0|A0A417PSY0_9CLOT Cell division protein FtsW OS=Clostridiaceae bacterium OM08-6BH OX=2292274 GN=DXC26_02850 PE=4 SV=1
MM1 pKa = 7.24KK2 pKa = 10.06RR3 pKa = 11.84KK4 pKa = 9.52LVALSLAVIMTAALTACGEE23 pKa = 4.2NSSSGTEE30 pKa = 4.01TQEE33 pKa = 4.1EE34 pKa = 4.82EE35 pKa = 4.23TTASEE40 pKa = 4.19EE41 pKa = 4.49TEE43 pKa = 4.31GTDD46 pKa = 3.31LQAAIVDD53 pKa = 3.36IDD55 pKa = 4.08EE56 pKa = 4.75APEE59 pKa = 3.96LTGSYY64 pKa = 10.08TIGLVIDD71 pKa = 4.01NNSDD75 pKa = 3.47QFNHH79 pKa = 6.5EE80 pKa = 4.25EE81 pKa = 4.32GEE83 pKa = 4.16AMKK86 pKa = 10.82ALGAAYY92 pKa = 10.17SSADD96 pKa = 3.33VTVTVMDD103 pKa = 3.98GQGDD107 pKa = 3.94VMKK110 pKa = 10.72QNQCVEE116 pKa = 3.98DD117 pKa = 4.76LINKK121 pKa = 9.1GVDD124 pKa = 4.03LILIIPIDD132 pKa = 3.85ADD134 pKa = 3.86GNVPAVNEE142 pKa = 3.99ALEE145 pKa = 4.2AGIPVVALDD154 pKa = 3.7ADD156 pKa = 4.33VNVEE160 pKa = 4.75DD161 pKa = 4.82DD162 pKa = 3.08MKK164 pKa = 10.9YY165 pKa = 10.18YY166 pKa = 10.92VGASDD171 pKa = 3.95YY172 pKa = 11.4NAGKK176 pKa = 9.91AQAEE180 pKa = 4.37YY181 pKa = 10.1LAEE184 pKa = 4.07VLPEE188 pKa = 3.94NANVLVILGQQGMSNAQNRR207 pKa = 11.84RR208 pKa = 11.84DD209 pKa = 3.71GAVEE213 pKa = 3.78TLTEE217 pKa = 4.16LRR219 pKa = 11.84PDD221 pKa = 3.35VTFLAEE227 pKa = 5.09QPGDD231 pKa = 3.38WDD233 pKa = 3.45EE234 pKa = 4.33AKK236 pKa = 10.79CMEE239 pKa = 5.0IVEE242 pKa = 4.86DD243 pKa = 3.37WCQAYY248 pKa = 10.04DD249 pKa = 3.44QFDD252 pKa = 4.61AIICGGDD259 pKa = 3.45SMALGAEE266 pKa = 4.31EE267 pKa = 5.12ALEE270 pKa = 3.99QADD273 pKa = 4.46RR274 pKa = 11.84LDD276 pKa = 3.42GVYY279 pKa = 8.65ITGVDD284 pKa = 4.41CIDD287 pKa = 3.53QVVALMKK294 pKa = 9.92EE295 pKa = 4.44GKK297 pKa = 9.51IAMSAQHH304 pKa = 5.92TADD307 pKa = 3.06MCAEE311 pKa = 4.01KK312 pKa = 10.73GFNYY316 pKa = 10.2ALHH319 pKa = 6.0VLNGEE324 pKa = 4.2NPDD327 pKa = 3.76DD328 pKa = 4.61YY329 pKa = 10.38IWEE332 pKa = 4.17YY333 pKa = 11.16VAVNKK338 pKa = 10.87DD339 pKa = 3.52NVNEE343 pKa = 4.63FYY345 pKa = 10.6PDD347 pKa = 2.98

Molecular weight: 37.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3R6SUC2|A0A3R6SUC2_9CLOT Uncharacterized protein OS=Clostridiaceae bacterium OM08-6BH OX=2292274 GN=DXC26_11380 PE=4 SV=1
MM1 pKa = 7.16RR2 pKa = 11.84TLRR5 pKa = 11.84QKK7 pKa = 10.37MSLARR12 pKa = 11.84DD13 pKa = 3.59QRR15 pKa = 11.84RR16 pKa = 11.84HH17 pKa = 5.31LSQNRR22 pKa = 11.84RR23 pKa = 11.84NQNQRR28 pKa = 11.84SQNRR32 pKa = 11.84RR33 pKa = 11.84NQNQRR38 pKa = 11.84NWNRR42 pKa = 11.84RR43 pKa = 11.84NQNQRR48 pKa = 11.84NRR50 pKa = 11.84NQRR53 pKa = 11.84NQNQRR58 pKa = 11.84NRR60 pKa = 11.84NWRR63 pKa = 11.84RR64 pKa = 2.92

Molecular weight: 8.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3400

0

3400

1057526

25

4441

311.0

34.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.68 ± 0.043

1.53 ± 0.017

5.643 ± 0.036

7.838 ± 0.048

3.942 ± 0.033

7.138 ± 0.044

1.833 ± 0.019

6.861 ± 0.036

6.608 ± 0.034

9.13 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.188 ± 0.027

4.06 ± 0.032

3.426 ± 0.024

3.479 ± 0.026

4.562 ± 0.046

5.634 ± 0.035

5.523 ± 0.052

6.866 ± 0.037

0.954 ± 0.016

4.104 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski