Pteromalus puparum negative-strand RNA virus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Artoviridae; Peropuvirus; Pteromalus puparum peropuvirus

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L5BWP3|A0A1L5BWP3_9MONO Uncharacterized protein OS=Pteromalus puparum negative-strand RNA virus 1 OX=1926633 PE=4 SV=1
MM1 pKa = 7.3PVVVPSNSAMAFTYY15 pKa = 11.11ADD17 pKa = 4.75LNPLLQAAGFPSIPDD32 pKa = 3.33NTTSWRR38 pKa = 11.84FTSYY42 pKa = 11.07KK43 pKa = 10.67DD44 pKa = 3.8LISFRR49 pKa = 11.84ARR51 pKa = 11.84ILPTTIGMMIDD62 pKa = 3.23QATPPRR68 pKa = 11.84VDD70 pKa = 2.89GAQFEE75 pKa = 5.02QNSSTAPPNSYY86 pKa = 10.5LYY88 pKa = 10.69FIATVLKK95 pKa = 9.66RR96 pKa = 11.84TDD98 pKa = 2.84VDD100 pKa = 3.64YY101 pKa = 11.28TGILVAVGLPQFCASVKK118 pKa = 9.5RR119 pKa = 11.84TLLSLQPEE127 pKa = 4.46LEE129 pKa = 3.89ILANEE134 pKa = 4.47DD135 pKa = 3.32EE136 pKa = 4.9LNRR139 pKa = 11.84FPPLTGIRR147 pKa = 11.84QFTSLGATEE156 pKa = 4.55LTCFLASEE164 pKa = 4.2DD165 pKa = 3.82HH166 pKa = 6.62LFQILEE172 pKa = 4.3DD173 pKa = 3.52QTISQEE179 pKa = 4.28SRR181 pKa = 11.84VCTALVAMFPYY192 pKa = 10.34ILPEE196 pKa = 3.95LQTRR200 pKa = 11.84VHH202 pKa = 6.27VVHH205 pKa = 7.17TYY207 pKa = 10.21QGGAQVRR214 pKa = 11.84PEE216 pKa = 3.8ALFGGGAEE224 pKa = 3.94QAVEE228 pKa = 3.75NARR231 pKa = 11.84RR232 pKa = 11.84LTLLAIYY239 pKa = 10.02LLAPAYY245 pKa = 8.36TRR247 pKa = 11.84QDD249 pKa = 3.31KK250 pKa = 10.63LHH252 pKa = 5.78EE253 pKa = 4.41QIARR257 pKa = 11.84RR258 pKa = 11.84TQAAAVQEE266 pKa = 4.18QLEE269 pKa = 4.77HH270 pKa = 6.47YY271 pKa = 8.54DD272 pKa = 3.24ASRR275 pKa = 11.84TRR277 pKa = 11.84TLLDD281 pKa = 3.18SLQWKK286 pKa = 8.52IGSMMPIVDD295 pKa = 4.74DD296 pKa = 3.77VLAMVICRR304 pKa = 11.84TTRR307 pKa = 11.84GTTDD311 pKa = 3.21PNAPLTEE318 pKa = 5.11LISPGEE324 pKa = 3.88LNRR327 pKa = 11.84NQEE330 pKa = 3.99IGRR333 pKa = 11.84VASSLLEE340 pKa = 4.01QARR343 pKa = 11.84LVYY346 pKa = 10.05TNYY349 pKa = 10.14HH350 pKa = 5.08STSIHH355 pKa = 6.57FILPVFDD362 pKa = 4.89PLVTAIGADD371 pKa = 2.81HH372 pKa = 6.27LTFRR376 pKa = 11.84EE377 pKa = 4.12EE378 pKa = 3.34ITRR381 pKa = 11.84FRR383 pKa = 11.84GIRR386 pKa = 11.84TVVSSAPFMGLCAQLPAQYY405 pKa = 10.03HH406 pKa = 5.94IKK408 pKa = 10.3NCARR412 pKa = 11.84LAYY415 pKa = 10.38LGLLYY420 pKa = 10.19HH421 pKa = 6.98DD422 pKa = 5.14RR423 pKa = 11.84SLTTPEE429 pKa = 3.79EE430 pKa = 3.91KK431 pKa = 10.68ASFKK435 pKa = 10.24EE436 pKa = 4.12YY437 pKa = 10.22KK438 pKa = 9.57ISGVRR443 pKa = 11.84EE444 pKa = 4.38HH445 pKa = 7.04IASQADD451 pKa = 3.89RR452 pKa = 11.84NIVEE456 pKa = 4.33NLVDD460 pKa = 3.92ILPSQTVSAIASLVQHH476 pKa = 6.77ISLEE480 pKa = 4.19KK481 pKa = 10.68ASILMDD487 pKa = 3.63SKK489 pKa = 11.42SPEE492 pKa = 3.89EE493 pKa = 3.76QLEE496 pKa = 4.15VLNLLRR502 pKa = 11.84GEE504 pKa = 4.47DD505 pKa = 4.28APGAWAMDD513 pKa = 3.29QMNRR517 pKa = 11.84EE518 pKa = 3.85NATYY522 pKa = 9.24TRR524 pKa = 11.84QLIEE528 pKa = 4.55SGVKK532 pKa = 9.68LLKK535 pKa = 10.46AALEE539 pKa = 4.08EE540 pKa = 4.23QYY542 pKa = 11.14EE543 pKa = 4.18LRR545 pKa = 11.84RR546 pKa = 11.84DD547 pKa = 3.56AAEE550 pKa = 4.02TLPTPDD556 pKa = 2.82AVRR559 pKa = 11.84TRR561 pKa = 11.84RR562 pKa = 11.84AALIEE567 pKa = 3.83WRR569 pKa = 11.84KK570 pKa = 10.48GITNMFSNLTDD581 pKa = 3.09WTEE584 pKa = 4.32ALPSAPRR591 pKa = 11.84SGMEE595 pKa = 4.77AIRR598 pKa = 11.84TTTLARR604 pKa = 11.84LTEE607 pKa = 3.97IMTAIRR613 pKa = 11.84TDD615 pKa = 3.31DD616 pKa = 3.86AMEE619 pKa = 3.79QQ620 pKa = 3.35

Molecular weight: 69.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L5BWP9|A0A1L5BWP9_9MONO GDP polyribonucleotidyltransferase OS=Pteromalus puparum negative-strand RNA virus 1 OX=1926633 PE=4 SV=1
MM1 pKa = 7.46SGKK4 pKa = 10.02KK5 pKa = 9.39DD6 pKa = 3.27AKK8 pKa = 9.41TPRR11 pKa = 11.84SATKK15 pKa = 8.89GTLSKK20 pKa = 11.17AEE22 pKa = 4.29EE23 pKa = 4.27LLQQLAAYY31 pKa = 9.53DD32 pKa = 4.13PLTNNDD38 pKa = 4.15PLSLGKK44 pKa = 10.02KK45 pKa = 8.8RR46 pKa = 11.84PKK48 pKa = 9.91PPKK51 pKa = 10.27CPDD54 pKa = 2.89IQEE57 pKa = 4.89PSDD60 pKa = 3.52SPPYY64 pKa = 9.59KK65 pKa = 10.31RR66 pKa = 11.84HH67 pKa = 5.71DD68 pKa = 3.7TRR70 pKa = 11.84GDD72 pKa = 3.53EE73 pKa = 4.33PDD75 pKa = 3.33EE76 pKa = 4.37TRR78 pKa = 11.84DD79 pKa = 3.92PDD81 pKa = 4.15PSLHH85 pKa = 6.16PCYY88 pKa = 10.43YY89 pKa = 9.93PLGNSSSTASIVEE102 pKa = 4.43VIEE105 pKa = 5.57SDD107 pKa = 4.04SQDD110 pKa = 3.58LNPPEE115 pKa = 4.19EE116 pKa = 4.51PKK118 pKa = 10.77DD119 pKa = 3.83RR120 pKa = 11.84PSTRR124 pKa = 11.84GEE126 pKa = 3.94TSRR129 pKa = 11.84TATRR133 pKa = 11.84EE134 pKa = 3.7QRR136 pKa = 11.84EE137 pKa = 4.08VLSIRR142 pKa = 11.84KK143 pKa = 8.65LADD146 pKa = 3.33TQEE149 pKa = 4.53VISSDD154 pKa = 3.45EE155 pKa = 4.1EE156 pKa = 4.25EE157 pKa = 4.31EE158 pKa = 4.31GRR160 pKa = 11.84EE161 pKa = 3.98SGAVPKK167 pKa = 10.66GFYY170 pKa = 9.31TVPPPVLQYY179 pKa = 9.6ITGEE183 pKa = 4.18SSKK186 pKa = 10.96KK187 pKa = 9.71PGGFQTEE194 pKa = 3.77MDD196 pKa = 3.08RR197 pKa = 11.84WFEE200 pKa = 4.17DD201 pKa = 3.03EE202 pKa = 3.98WGKK205 pKa = 10.34LVNPPPGLLSTHH217 pKa = 6.14YY218 pKa = 9.84LWCLPPSTTNTLAGEE233 pKa = 4.21KK234 pKa = 10.42FKK236 pKa = 11.32VASNNLAISLGRR248 pKa = 11.84SKK250 pKa = 10.23IWGKK254 pKa = 10.51KK255 pKa = 8.98KK256 pKa = 10.18SSKK259 pKa = 8.92GTAGVLVLPFPGVQSNTAEE278 pKa = 4.21KK279 pKa = 10.55AAGDD283 pKa = 4.18RR284 pKa = 11.84LTKK287 pKa = 10.36AAEE290 pKa = 4.09ALQQLAEE297 pKa = 4.4DD298 pKa = 4.77FKK300 pKa = 11.54SAIAILQADD309 pKa = 4.51INRR312 pKa = 11.84QAAASRR318 pKa = 11.84GFITDD323 pKa = 3.42SSQSIRR329 pKa = 11.84ANTLSLIRR337 pKa = 11.84SMEE340 pKa = 3.95EE341 pKa = 3.31TAKK344 pKa = 10.46SVKK347 pKa = 9.33GTTTPVQGMGEE358 pKa = 4.11SRR360 pKa = 11.84RR361 pKa = 11.84DD362 pKa = 3.39SRR364 pKa = 11.84TTTPSLPGPGAGQSSRR380 pKa = 11.84PSTGGLTIDD389 pKa = 3.28PSKK392 pKa = 10.64FKK394 pKa = 10.73RR395 pKa = 11.84IVPQGKK401 pKa = 9.04KK402 pKa = 9.82

Molecular weight: 43.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

3913

151

2166

782.6

87.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.645 ± 0.951

1.508 ± 0.277

4.983 ± 0.309

5.929 ± 0.613

3.271 ± 0.706

5.929 ± 0.568

2.326 ± 0.505

6.21 ± 0.444

4.779 ± 0.694

11.756 ± 0.999

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.249 ± 0.237

3.603 ± 0.25

5.443 ± 0.79

4.651 ± 0.132

5.597 ± 0.489

7.335 ± 0.865

7.105 ± 0.985

5.955 ± 0.573

1.482 ± 0.353

3.246 ± 0.535
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski