Circular genetic element sp.

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A385E3D0|A0A385E3D0_9VIRU ATP-dependent helicase Rep OS=Circular genetic element sp. OX=2202954 PE=3 SV=1
MM1 pKa = 6.76QQQEE5 pKa = 4.5TTRR8 pKa = 11.84SRR10 pKa = 11.84NWCFTWNNPSEE21 pKa = 4.43SDD23 pKa = 3.71LVHH26 pKa = 6.43LRR28 pKa = 11.84NILKK32 pKa = 10.13HH33 pKa = 6.1KK34 pKa = 8.57RR35 pKa = 11.84VKK37 pKa = 10.06YY38 pKa = 9.64GVYY41 pKa = 10.32GMEE44 pKa = 4.09VSPTTGTPHH53 pKa = 5.93AQGYY57 pKa = 7.11VQFSQPQTFKK67 pKa = 11.27AMKK70 pKa = 9.65KK71 pKa = 9.68ALPPAHH77 pKa = 6.94IEE79 pKa = 4.05AAKK82 pKa = 8.66GTPKK86 pKa = 10.31QNQEE90 pKa = 4.03YY91 pKa = 7.9CTKK94 pKa = 10.87DD95 pKa = 3.04GDD97 pKa = 4.17SEE99 pKa = 4.65EE100 pKa = 5.16FGTPPKK106 pKa = 10.66GAGSRR111 pKa = 11.84SDD113 pKa = 5.29LEE115 pKa = 4.26ACVDD119 pKa = 5.01LIKK122 pKa = 10.9AGASMQTIAEE132 pKa = 4.21EE133 pKa = 4.91CPCQVVKK140 pKa = 10.63FGRR143 pKa = 11.84GLRR146 pKa = 11.84DD147 pKa = 3.63LKK149 pKa = 11.24LLLAKK154 pKa = 10.07PYY156 pKa = 8.31THH158 pKa = 7.07HH159 pKa = 7.56DD160 pKa = 3.73VRR162 pKa = 11.84GTWIWGPPGTGKK174 pKa = 9.9SHH176 pKa = 6.01TARR179 pKa = 11.84IQFPDD184 pKa = 4.13AFIKK188 pKa = 9.75PQNKK192 pKa = 7.96WWDD195 pKa = 3.63GYY197 pKa = 11.49ANEE200 pKa = 4.81DD201 pKa = 3.93TVILDD206 pKa = 4.73DD207 pKa = 6.59LDD209 pKa = 4.05TQTLGHH215 pKa = 6.06YY216 pKa = 10.3LKK218 pKa = 10.28IWTDD222 pKa = 3.26KK223 pKa = 10.97YY224 pKa = 10.7ATTGEE229 pKa = 4.54TKK231 pKa = 10.63GGTINLQYY239 pKa = 10.71KK240 pKa = 9.89RR241 pKa = 11.84FIITSNYY248 pKa = 8.69TPHH251 pKa = 6.51QLWPDD256 pKa = 3.89DD257 pKa = 4.04PVMCAAIKK265 pKa = 9.74RR266 pKa = 11.84RR267 pKa = 11.84CVMFHH272 pKa = 6.47KK273 pKa = 10.03ATRR276 pKa = 11.84EE277 pKa = 3.88NEE279 pKa = 4.2HH280 pKa = 7.58VIEE283 pKa = 4.26

Molecular weight: 32.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A385E3D0|A0A385E3D0_9VIRU ATP-dependent helicase Rep OS=Circular genetic element sp. OX=2202954 PE=3 SV=1
MM1 pKa = 6.76QQQEE5 pKa = 4.5TTRR8 pKa = 11.84SRR10 pKa = 11.84NWCFTWNNPSEE21 pKa = 4.43SDD23 pKa = 3.71LVHH26 pKa = 6.43LRR28 pKa = 11.84NILKK32 pKa = 10.13HH33 pKa = 6.1KK34 pKa = 8.57RR35 pKa = 11.84VKK37 pKa = 10.06YY38 pKa = 9.64GVYY41 pKa = 10.32GMEE44 pKa = 4.09VSPTTGTPHH53 pKa = 5.93AQGYY57 pKa = 7.11VQFSQPQTFKK67 pKa = 11.27AMKK70 pKa = 9.65KK71 pKa = 9.68ALPPAHH77 pKa = 6.94IEE79 pKa = 4.05AAKK82 pKa = 8.66GTPKK86 pKa = 10.31QNQEE90 pKa = 4.03YY91 pKa = 7.9CTKK94 pKa = 10.87DD95 pKa = 3.04GDD97 pKa = 4.17SEE99 pKa = 4.65EE100 pKa = 5.16FGTPPKK106 pKa = 10.66GAGSRR111 pKa = 11.84SDD113 pKa = 5.29LEE115 pKa = 4.26ACVDD119 pKa = 5.01LIKK122 pKa = 10.9AGASMQTIAEE132 pKa = 4.21EE133 pKa = 4.91CPCQVVKK140 pKa = 10.63FGRR143 pKa = 11.84GLRR146 pKa = 11.84DD147 pKa = 3.63LKK149 pKa = 11.24LLLAKK154 pKa = 10.07PYY156 pKa = 8.31THH158 pKa = 7.07HH159 pKa = 7.56DD160 pKa = 3.73VRR162 pKa = 11.84GTWIWGPPGTGKK174 pKa = 9.9SHH176 pKa = 6.01TARR179 pKa = 11.84IQFPDD184 pKa = 4.13AFIKK188 pKa = 9.75PQNKK192 pKa = 7.96WWDD195 pKa = 3.63GYY197 pKa = 11.49ANEE200 pKa = 4.81DD201 pKa = 3.93TVILDD206 pKa = 4.73DD207 pKa = 6.59LDD209 pKa = 4.05TQTLGHH215 pKa = 6.06YY216 pKa = 10.3LKK218 pKa = 10.28IWTDD222 pKa = 3.26KK223 pKa = 10.97YY224 pKa = 10.7ATTGEE229 pKa = 4.54TKK231 pKa = 10.63GGTINLQYY239 pKa = 10.71KK240 pKa = 9.89RR241 pKa = 11.84FIITSNYY248 pKa = 8.69TPHH251 pKa = 6.51QLWPDD256 pKa = 3.89DD257 pKa = 4.04PVMCAAIKK265 pKa = 9.74RR266 pKa = 11.84RR267 pKa = 11.84CVMFHH272 pKa = 6.47KK273 pKa = 10.03ATRR276 pKa = 11.84EE277 pKa = 3.88NEE279 pKa = 4.2HH280 pKa = 7.58VIEE283 pKa = 4.26

Molecular weight: 32.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1

0

1

283

283

283

283.0

32.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.714 ± 0.0

2.473 ± 0.0

5.654 ± 0.0

5.3 ± 0.0

3.18 ± 0.0

7.42 ± 0.0

3.887 ± 0.0

4.947 ± 0.0

8.127 ± 0.0

6.007 ± 0.0

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.12 ± 0.0

3.534 ± 0.0

6.36 ± 0.0

5.654 ± 0.0

4.594 ± 0.0

3.887 ± 0.0

9.187 ± 0.0

4.594 ± 0.0

2.827 ± 0.0

3.534 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski