delta proteobacterium NaphS2

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; unclassified Deltaproteobacteria

Average proteome isoelectric point is 7.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6687 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D8F3A8|D8F3A8_9DELT Dehydrogenase FMN-dependent OS=delta proteobacterium NaphS2 OX=88274 GN=NPH_3944 PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 9.05RR3 pKa = 11.84TTSVPPTYY11 pKa = 7.69TTKK14 pKa = 10.95ASNVSTALAGYY25 pKa = 8.72FAGGTGGAQSIDD37 pKa = 3.48TSEE40 pKa = 4.49GSPSVNSWKK49 pKa = 10.77GAGFQKK55 pKa = 10.09DD56 pKa = 3.35TTYY59 pKa = 10.38TANTAPYY66 pKa = 9.39FQITVDD72 pKa = 3.32TSKK75 pKa = 9.7YY76 pKa = 7.29TDD78 pKa = 3.46VIISLRR84 pKa = 11.84SYY86 pKa = 9.73ATANWGTSNVLKK98 pKa = 10.41VWSSADD104 pKa = 3.05GGTSFTATTPATGTLTKK121 pKa = 8.09TTWSGDD127 pKa = 3.4QTFAAAATGAVTTIFRR143 pKa = 11.84INAEE147 pKa = 4.09GANPTNGAAMMLDD160 pKa = 3.9NILVTGCGVPTPPPTITKK178 pKa = 10.06SFSPDD183 pKa = 3.14PTIKK187 pKa = 10.75GGTSTLTFTINNTEE201 pKa = 4.09SGNQALSGVAFTDD214 pKa = 3.54VLPEE218 pKa = 4.06GLFVADD224 pKa = 4.68DD225 pKa = 3.98TSPQCGGTLTTTAATRR241 pKa = 11.84TIALTGGTLAAGGNCTFDD259 pKa = 3.34VSVTGTLEE267 pKa = 3.92GQYY270 pKa = 10.57EE271 pKa = 4.24NVSGFISSNEE281 pKa = 4.23SGTSTHH287 pKa = 6.38YY288 pKa = 10.27ATDD291 pKa = 3.78SLNVIAPSILAKK303 pKa = 10.53SFAPASLLTGGSSTLTFSLTNPNQSGTLSGIGFTDD338 pKa = 3.92TLPAGLTVASGGPTAVCDD356 pKa = 3.91GTLTTTSPDD365 pKa = 3.59SISFSGGSLAANTTCTFDD383 pKa = 3.6VPVSGVTSGSKK394 pKa = 10.89ANTTGIITSTEE405 pKa = 3.93GGNGSSADD413 pKa = 3.31ATLNVSDD420 pKa = 4.39PVALIGLNKK429 pKa = 9.89EE430 pKa = 3.96VSTDD434 pKa = 3.02GTHH437 pKa = 6.02WYY439 pKa = 10.1KK440 pKa = 10.73FVGVPVGGNVYY451 pKa = 10.62YY452 pKa = 10.87RR453 pKa = 11.84FTVFNDD459 pKa = 4.03GEE461 pKa = 4.43ATLSSIDD468 pKa = 3.66VADD471 pKa = 4.28PDD473 pKa = 4.18ISLSSCSFPSSLAAGEE489 pKa = 4.51NPVSCTVGPITAVSGLHH506 pKa = 7.11DD507 pKa = 3.89NTATATGTYY516 pKa = 10.33GSQTPSDD523 pKa = 3.75TSTARR528 pKa = 11.84YY529 pKa = 9.42GSAEE533 pKa = 3.85LTIAKK538 pKa = 9.89SVTEE542 pKa = 3.9NDD544 pKa = 3.07FTTVGDD550 pKa = 3.87VLHH553 pKa = 6.18YY554 pKa = 10.66SYY556 pKa = 11.45LVTNSGSAPLLGPVTVSDD574 pKa = 4.48DD575 pKa = 3.6KK576 pKa = 12.09ANDD579 pKa = 3.77EE580 pKa = 4.35YY581 pKa = 11.02CPPVSTVGDD590 pKa = 3.89LDD592 pKa = 4.23NYY594 pKa = 10.47LDD596 pKa = 4.22PGEE599 pKa = 5.59SITCTAMYY607 pKa = 9.27TIEE610 pKa = 4.46SSDD613 pKa = 3.37IASGSVTNTATATADD628 pKa = 3.34GVTSNVDD635 pKa = 3.29SKK637 pKa = 10.06TVTSEE642 pKa = 4.16SIADD646 pKa = 3.64LSITKK651 pKa = 10.31SSTPDD656 pKa = 3.25PYY658 pKa = 11.52VPGDD662 pKa = 3.4GFTYY666 pKa = 9.96TIVVSNAGPSDD677 pKa = 3.58VTDD680 pKa = 4.04ARR682 pKa = 11.84VQDD685 pKa = 4.41ALPAALSGFTWTCTPNGVGAVCGSGSGTGDD715 pKa = 2.51IDD717 pKa = 4.72AFVTLPSGTSAIFSVSGTVPSGTTGDD743 pKa = 4.03LVNTATVQPPAGITDD758 pKa = 4.29PVPGSNSDD766 pKa = 3.18SDD768 pKa = 4.3TNPSGSAVSDD778 pKa = 3.5LSITKK783 pKa = 10.31SSTPDD788 pKa = 3.25PYY790 pKa = 11.52VPGDD794 pKa = 3.4GFTYY798 pKa = 9.96TIVVSNAGPSDD809 pKa = 3.58VTDD812 pKa = 4.04ARR814 pKa = 11.84VQDD817 pKa = 4.41ALPAALSGFAWTCTSNGGGAVCGSAGGTGDD847 pKa = 2.79IDD849 pKa = 4.33AFVSLPAGTSVNFSVTGTVPSGTTGSLMNTATIQPPSGTTDD890 pKa = 3.07PVTGNNSDD898 pKa = 3.64SDD900 pKa = 4.3TNPGGGPAIPTTNEE914 pKa = 3.27YY915 pKa = 11.35GLIFLAILLVLFSLNHH931 pKa = 6.68LGRR934 pKa = 11.84QRR936 pKa = 11.84RR937 pKa = 11.84II938 pKa = 3.29

Molecular weight: 93.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D8F2J8|D8F2J8_9DELT Uncharacterized protein OS=delta proteobacterium NaphS2 OX=88274 GN=NPH_0585 PE=4 SV=1
MM1 pKa = 7.4NVRR4 pKa = 11.84KK5 pKa = 10.0INAIKK10 pKa = 9.44HH11 pKa = 5.1QKK13 pKa = 9.3NKK15 pKa = 10.14SIPIFRR21 pKa = 11.84SNINAIVRR29 pKa = 11.84NAFVGKK35 pKa = 10.05RR36 pKa = 11.84AFKK39 pKa = 10.74

Molecular weight: 4.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6687

0

6687

1789710

30

2881

267.6

30.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.799 ± 0.03

1.366 ± 0.013

5.315 ± 0.023

6.659 ± 0.034

4.518 ± 0.023

7.426 ± 0.03

2.24 ± 0.015

6.442 ± 0.024

6.361 ± 0.03

9.97 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.83 ± 0.015

3.654 ± 0.02

4.58 ± 0.019

3.178 ± 0.019

6.204 ± 0.03

5.721 ± 0.021

4.85 ± 0.023

6.677 ± 0.025

1.204 ± 0.013

3.002 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski