Enterobacter cloacae S611

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex; Enterobacter cloacae

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3989 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V5CS76|V5CS76_ENTCL Metallo-beta-lactamase superfamily protein OS=Enterobacter cloacae S611 OX=1399146 GN=EDP2_2532 PE=4 SV=1
MM1 pKa = 7.64SDD3 pKa = 4.45DD4 pKa = 3.53VALPLQFTDD13 pKa = 3.4AAANKK18 pKa = 8.91VKK20 pKa = 10.8SLIADD25 pKa = 3.6EE26 pKa = 4.73EE27 pKa = 4.41NPNLKK32 pKa = 10.23LRR34 pKa = 11.84VYY36 pKa = 9.05ITGGGCSGFQYY47 pKa = 11.03GFTFDD52 pKa = 4.96DD53 pKa = 4.21QINDD57 pKa = 3.16GDD59 pKa = 4.0MTIEE63 pKa = 3.95KK64 pKa = 10.11QGVGLVVDD72 pKa = 4.57PMSLQYY78 pKa = 11.16LVGGSVDD85 pKa = 3.52YY86 pKa = 11.39TEE88 pKa = 4.94GLEE91 pKa = 4.08GSRR94 pKa = 11.84FVVTNPNATSTCGCGSSFSII114 pKa = 5.02

Molecular weight: 12.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V5A5R5|V5A5R5_ENTCL Sensory box protein OS=Enterobacter cloacae S611 OX=1399146 GN=EDP2_2407 PE=4 SV=1
MM1 pKa = 7.91RR2 pKa = 11.84RR3 pKa = 11.84RR4 pKa = 11.84KK5 pKa = 8.39QRR7 pKa = 11.84KK8 pKa = 6.61RR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 7.98PQQTRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84KK21 pKa = 9.02RR22 pKa = 11.84RR23 pKa = 11.84RR24 pKa = 11.84MQPKK28 pKa = 8.45PPPMHH33 pKa = 6.29RR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 9.83PKK38 pKa = 9.54RR39 pKa = 11.84RR40 pKa = 11.84PRR42 pKa = 11.84KK43 pKa = 8.84PPLTHH48 pKa = 6.81RR49 pKa = 11.84RR50 pKa = 11.84KK51 pKa = 9.91PKK53 pKa = 8.93QKK55 pKa = 9.06PRR57 pKa = 11.84KK58 pKa = 7.3RR59 pKa = 11.84QPMRR63 pKa = 11.84RR64 pKa = 11.84RR65 pKa = 11.84KK66 pKa = 9.59RR67 pKa = 11.84RR68 pKa = 11.84RR69 pKa = 11.84KK70 pKa = 9.31RR71 pKa = 11.84LNRR74 pKa = 11.84LLLKK78 pKa = 10.29KK79 pKa = 8.93PQPRR83 pKa = 11.84RR84 pKa = 11.84PPPLKK89 pKa = 10.2KK90 pKa = 10.38LPQRR94 pKa = 11.84RR95 pKa = 11.84PLPQRR100 pKa = 11.84KK101 pKa = 7.06PQRR104 pKa = 11.84RR105 pKa = 11.84RR106 pKa = 11.84KK107 pKa = 8.54PLQRR111 pKa = 11.84KK112 pKa = 7.64KK113 pKa = 9.56QQPRR117 pKa = 11.84RR118 pKa = 11.84KK119 pKa = 9.19RR120 pKa = 11.84LRR122 pKa = 11.84KK123 pKa = 9.63KK124 pKa = 9.74PPLRR128 pKa = 11.84RR129 pKa = 11.84KK130 pKa = 9.79LPPRR134 pKa = 11.84RR135 pKa = 11.84PLPIRR140 pKa = 11.84KK141 pKa = 9.0RR142 pKa = 11.84LPKK145 pKa = 10.11KK146 pKa = 8.72PQQRR150 pKa = 11.84KK151 pKa = 8.46PPPLKK156 pKa = 10.71ASTICLAISARR167 pKa = 11.84VRR169 pKa = 11.84MHH171 pKa = 7.17RR172 pKa = 11.84KK173 pKa = 8.86RR174 pKa = 11.84AAEE177 pKa = 4.13RR178 pKa = 11.84KK179 pKa = 8.38ATMQRR184 pKa = 11.84RR185 pKa = 11.84RR186 pKa = 11.84VRR188 pKa = 11.84VTLRR192 pKa = 11.84TTVHH196 pKa = 7.01PEE198 pKa = 4.28PISVTTQGRR207 pKa = 11.84LKK209 pKa = 10.48RR210 pKa = 11.84RR211 pKa = 11.84LRR213 pKa = 11.84AASMMHH219 pKa = 7.03PPTPGRR225 pKa = 11.84PVRR228 pKa = 11.84YY229 pKa = 9.2VSNWLLMGRR238 pKa = 11.84CC239 pKa = 3.8

Molecular weight: 29.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3989

0

3989

1290228

29

4981

323.4

35.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.278 ± 0.042

1.029 ± 0.015

5.13 ± 0.029

5.707 ± 0.038

3.878 ± 0.03

7.404 ± 0.043

2.292 ± 0.02

5.526 ± 0.033

4.001 ± 0.038

11.081 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.674 ± 0.02

3.628 ± 0.035

4.487 ± 0.035

4.561 ± 0.029

5.862 ± 0.033

5.883 ± 0.037

5.289 ± 0.035

7.071 ± 0.033

1.548 ± 0.02

2.67 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski