Aminobacter sp. DSM 101952

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Phyllobacteriaceae; Aminobacter; unclassified Aminobacter

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4851 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q6LLH8|A0A0Q6LLH8_9RHIZ Spermidine/putrescine ABC transporter permease OS=Aminobacter sp. DSM 101952 OX=2735891 GN=ASC75_12690 PE=3 SV=1
MM1 pKa = 6.6YY2 pKa = 10.05AAYY5 pKa = 9.92FDD7 pKa = 5.29RR8 pKa = 11.84ISEE11 pKa = 4.52TIAHH15 pKa = 7.09FIGLFDD21 pKa = 3.82TASEE25 pKa = 4.1EE26 pKa = 4.19ARR28 pKa = 11.84LKK30 pKa = 9.74QAYY33 pKa = 10.06DD34 pKa = 3.3EE35 pKa = 4.95FRR37 pKa = 11.84AKK39 pKa = 9.91QQADD43 pKa = 3.29EE44 pKa = 4.95HH45 pKa = 7.83LPEE48 pKa = 4.87HH49 pKa = 5.36QTAALKK55 pKa = 10.7VDD57 pKa = 3.82TSHH60 pKa = 6.65TLEE63 pKa = 5.38DD64 pKa = 4.32FDD66 pKa = 5.87PSLKK70 pKa = 10.44YY71 pKa = 10.64VGVRR75 pKa = 11.84PEE77 pKa = 3.88IEE79 pKa = 4.42QITVWSKK86 pKa = 10.83VAFTPPDD93 pKa = 3.3VAVPDD98 pKa = 4.41GPFLQNYY105 pKa = 8.31AHH107 pKa = 7.43LPQGTVGSGGIVLPQILPPGDD128 pKa = 3.27LAVIVNQEE136 pKa = 3.56IRR138 pKa = 11.84LSDD141 pKa = 3.62DD142 pKa = 3.84DD143 pKa = 4.14YY144 pKa = 11.96VGFGGSGLKK153 pKa = 10.3FNAPPVDD160 pKa = 4.28DD161 pKa = 4.42SPLVEE166 pKa = 4.29MQQAAADD173 pKa = 4.46LSPIDD178 pKa = 5.51DD179 pKa = 3.94LTLPGSTEE187 pKa = 3.64EE188 pKa = 3.99MATFVTTAQARR199 pKa = 11.84FNDD202 pKa = 4.13FDD204 pKa = 6.66ADD206 pKa = 3.64DD207 pKa = 4.58HH208 pKa = 6.84GNANVFCVKK217 pKa = 10.69AEE219 pKa = 4.4TIEE222 pKa = 3.96GTYY225 pKa = 10.68FNGQLIDD232 pKa = 3.67EE233 pKa = 4.71ADD235 pKa = 3.56APKK238 pKa = 10.11MEE240 pKa = 5.64DD241 pKa = 3.31YY242 pKa = 11.29INFLKK247 pKa = 10.87EE248 pKa = 3.52QDD250 pKa = 4.51DD251 pKa = 4.07DD252 pKa = 5.04AKK254 pKa = 11.47GPDD257 pKa = 3.57APLDD261 pKa = 3.48VDD263 pKa = 4.33TPPDD267 pKa = 3.5SSDD270 pKa = 5.32FMDD273 pKa = 3.34GWGDD277 pKa = 3.4GTANPSVEE285 pKa = 3.74ISTGGNTVINNAVVVNDD302 pKa = 3.8WASAEE307 pKa = 3.98VMAVMGDD314 pKa = 4.12HH315 pKa = 6.91ISLNAIIQINGTCDD329 pKa = 3.51ADD331 pKa = 3.9SVGAAVGGWPFDD343 pKa = 4.06PGGGDD348 pKa = 3.25NNLSFNIAMFKK359 pKa = 10.7HH360 pKa = 6.58IDD362 pKa = 3.46TSGGDD367 pKa = 3.47QAPAEE372 pKa = 4.51APTGFPAYY380 pKa = 10.24YY381 pKa = 9.97VVTEE385 pKa = 4.08ISGDD389 pKa = 3.88LIMMNWIEE397 pKa = 4.12QYY399 pKa = 11.74AFVTDD404 pKa = 3.66NDD406 pKa = 3.73VVIASSSGVQSVVSTGDD423 pKa = 3.02NTAFNGLSLNEE434 pKa = 3.71IGNYY438 pKa = 9.02YY439 pKa = 10.61DD440 pKa = 5.58LIIIGGNVYY449 pKa = 10.18DD450 pKa = 4.84ASIIHH455 pKa = 6.28QINLLLDD462 pKa = 3.64NDD464 pKa = 4.18VIGAVSGFEE473 pKa = 4.16TTGEE477 pKa = 4.37GSVSTGDD484 pKa = 3.38NLLWNQASIVNVGNATAEE502 pKa = 4.1ALPDD506 pKa = 4.43GYY508 pKa = 11.52NKK510 pKa = 10.59AAGDD514 pKa = 3.64LAAGNKK520 pKa = 7.82EE521 pKa = 4.21LPDD524 pKa = 5.56SILQDD529 pKa = 3.17AAFAGIGVLRR539 pKa = 11.84VLYY542 pKa = 10.32ISGDD546 pKa = 3.49IYY548 pKa = 10.95NLNYY552 pKa = 9.86IKK554 pKa = 9.57QTTVVGDD561 pKa = 3.66SDD563 pKa = 4.13QVALAMNNLVANPDD577 pKa = 3.88AEE579 pKa = 4.4WTITTGGNQLVNDD592 pKa = 4.06ATIVDD597 pKa = 3.63VDD599 pKa = 4.17GTSKK603 pKa = 10.38IYY605 pKa = 10.93VGGDD609 pKa = 3.21SYY611 pKa = 11.88SDD613 pKa = 3.97EE614 pKa = 4.37ILIQANLVSSEE625 pKa = 4.32PDD627 pKa = 3.08LGAQNPDD634 pKa = 2.94ILVTEE639 pKa = 4.3AVVFLDD645 pKa = 5.53DD646 pKa = 5.33GGDD649 pKa = 3.76DD650 pKa = 3.94TGDD653 pKa = 3.38ASNTNPIAPQPADD666 pKa = 3.53SGSADD671 pKa = 3.45VMQHH675 pKa = 5.04MLGG678 pKa = 3.58

Molecular weight: 71.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q6LAC6|A0A0Q6LAC6_9RHIZ Amidase OS=Aminobacter sp. DSM 101952 OX=2735891 GN=ASC75_12330 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.11GGRR28 pKa = 11.84GVVAARR34 pKa = 11.84RR35 pKa = 11.84NRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4851

0

4851

1488852

29

3861

306.9

33.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.44 ± 0.052

0.787 ± 0.012

5.761 ± 0.037

5.73 ± 0.033

3.877 ± 0.026

8.667 ± 0.038

2.002 ± 0.017

5.347 ± 0.025

3.651 ± 0.031

9.848 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.588 ± 0.015

2.672 ± 0.023

4.928 ± 0.025

3.009 ± 0.02

6.816 ± 0.039

5.536 ± 0.023

5.192 ± 0.029

7.621 ± 0.026

1.307 ± 0.018

2.221 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski