Paenibacillus aquistagni

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4778 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X7I3H9|A0A1X7I3H9_9BACL Queuine tRNA-ribosyltransferase OS=Paenibacillus aquistagni OX=1852522 GN=tgt PE=3 SV=1
MM1 pKa = 7.2VNDD4 pKa = 4.01MRR6 pKa = 11.84CPNCNSKK13 pKa = 10.84DD14 pKa = 3.12IGKK17 pKa = 9.7IGSHH21 pKa = 6.05QFYY24 pKa = 10.47CWGCFIEE31 pKa = 4.51LTVNGDD37 pKa = 3.09KK38 pKa = 10.62MSVYY42 pKa = 10.04QVEE45 pKa = 4.22EE46 pKa = 4.6DD47 pKa = 3.68GTLSSLDD54 pKa = 3.75DD55 pKa = 4.63LFFGDD60 pKa = 4.99DD61 pKa = 3.7WLPTDD66 pKa = 3.66VGEE69 pKa = 4.63TQTSTQMM76 pKa = 3.47

Molecular weight: 8.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X7KKA9|A0A1X7KKA9_9BACL Two-component signal transduction system YycFG regulatory protein YycI OS=Paenibacillus aquistagni OX=1852522 GN=HII26_06980 PE=4 SV=1
MM1 pKa = 7.59KK2 pKa = 8.7PTFKK6 pKa = 10.81PNVSKK11 pKa = 10.8RR12 pKa = 11.84KK13 pKa = 8.79KK14 pKa = 8.3VHH16 pKa = 5.4GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.19NGRR28 pKa = 11.84KK29 pKa = 8.78VLAARR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.23GRR39 pKa = 11.84KK40 pKa = 8.56VLSAA44 pKa = 4.05

Molecular weight: 5.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4778

0

4778

1524220

29

6382

319.0

35.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.16 ± 0.034

0.834 ± 0.012

5.112 ± 0.028

7.03 ± 0.043

3.933 ± 0.028

6.893 ± 0.03

2.502 ± 0.023

6.835 ± 0.032

5.322 ± 0.033

10.085 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.157 ± 0.015

3.59 ± 0.025

3.893 ± 0.019

4.341 ± 0.029

4.954 ± 0.031

6.276 ± 0.03

5.236 ± 0.028

6.979 ± 0.03

1.291 ± 0.015

3.576 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski