Dyella sp. OK004

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Rhodanobacteraceae; Dyella; unclassified Dyella

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4204 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I6MG55|A0A1I6MG55_9GAMM Pseudouridine synthase OS=Dyella sp. OK004 OX=1855292 GN=SAMN05216570_3140 PE=3 SV=1
MM1 pKa = 7.62TDD3 pKa = 3.48DD4 pKa = 3.5TPKK7 pKa = 11.04YY8 pKa = 7.98MGIYY12 pKa = 9.94RR13 pKa = 11.84GTVCNCVDD21 pKa = 4.24PLNQGRR27 pKa = 11.84IQAFVTDD34 pKa = 3.71VCGDD38 pKa = 3.96TPSSWAMPCLPMTGIQAGIYY58 pKa = 9.28AVPPMDD64 pKa = 4.28AGVWMMFEE72 pKa = 4.97GGDD75 pKa = 3.31ADD77 pKa = 3.91RR78 pKa = 11.84PVWLGGWWGSRR89 pKa = 11.84AEE91 pKa = 4.34VPALAQAGNPLSPSIVLQTLGQNVISISDD120 pKa = 3.76LPGPAGGLLLRR131 pKa = 11.84SGTSMIMVNQAGITITNGQGATILMTGTEE160 pKa = 4.08VMINNGALMVSS171 pKa = 4.11

Molecular weight: 17.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I6KXD4|A0A1I6KXD4_9GAMM Uncharacterized conserved protein DUF885 familyt OS=Dyella sp. OK004 OX=1855292 GN=SAMN05216570_1290 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSKK10 pKa = 10.06LKK12 pKa = 10.38RR13 pKa = 11.84ARR15 pKa = 11.84THH17 pKa = 5.89GFRR20 pKa = 11.84ARR22 pKa = 11.84MATADD27 pKa = 3.2GRR29 pKa = 11.84KK30 pKa = 9.07ILNARR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 9.77GRR40 pKa = 11.84KK41 pKa = 8.92RR42 pKa = 11.84LIPP45 pKa = 4.02

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4204

0

4204

1469863

32

12747

349.6

38.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.177 ± 0.052

0.807 ± 0.012

5.628 ± 0.031

4.916 ± 0.049

3.396 ± 0.023

8.515 ± 0.058

2.467 ± 0.022

4.359 ± 0.028

3.087 ± 0.042

10.666 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.263 ± 0.02

2.968 ± 0.045

5.186 ± 0.029

3.966 ± 0.031

6.782 ± 0.058

5.829 ± 0.039

5.44 ± 0.054

7.349 ± 0.032

1.559 ± 0.019

2.641 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski