Hubei sobemo-like virus 32

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEM3|A0A1L3KEM3_9VIRU C2H2-type domain-containing protein OS=Hubei sobemo-like virus 32 OX=1923219 PE=4 SV=1
MM1 pKa = 6.95VCDD4 pKa = 4.85RR5 pKa = 11.84MVSCKK10 pKa = 7.71WRR12 pKa = 11.84IPSDD16 pKa = 3.39FLTRR20 pKa = 11.84GHH22 pKa = 6.8FDD24 pKa = 2.93RR25 pKa = 11.84VVRR28 pKa = 11.84EE29 pKa = 4.33LEE31 pKa = 4.17WTSSPGYY38 pKa = 8.44PYY40 pKa = 10.53CVRR43 pKa = 11.84HH44 pKa = 5.31PTNAQFFEE52 pKa = 4.63VVDD55 pKa = 4.27GQPSQIALDD64 pKa = 3.94RR65 pKa = 11.84VWYY68 pKa = 8.87MVVQRR73 pKa = 11.84LRR75 pKa = 11.84DD76 pKa = 3.58QTSDD80 pKa = 4.1PIRR83 pKa = 11.84LFIKK87 pKa = 10.6PEE89 pKa = 3.56AHH91 pKa = 7.07KK92 pKa = 10.15MSKK95 pKa = 10.29LLEE98 pKa = 3.8HH99 pKa = 7.75RR100 pKa = 11.84YY101 pKa = 10.07RR102 pKa = 11.84IISSVSVIDD111 pKa = 4.29QIIDD115 pKa = 3.14HH116 pKa = 6.33MLFQEE121 pKa = 4.22FNDD124 pKa = 3.24RR125 pKa = 11.84VVEE128 pKa = 4.12NVHH131 pKa = 5.62YY132 pKa = 10.95VPAKK136 pKa = 10.55VGWTPYY142 pKa = 9.5IGGWKK147 pKa = 9.33MIRR150 pKa = 11.84PHH152 pKa = 7.97GMVSADD158 pKa = 2.96KK159 pKa = 9.85TAWDD163 pKa = 3.46WTVPMWMLDD172 pKa = 2.85WEE174 pKa = 4.49FQVRR178 pKa = 11.84VKK180 pKa = 10.75LCEE183 pKa = 3.83NMTEE187 pKa = 3.83QWLDD191 pKa = 3.37LAAWRR196 pKa = 11.84YY197 pKa = 9.32NKK199 pKa = 10.39LFVDD203 pKa = 4.11VTLVTSGGLLLKK215 pKa = 10.36QRR217 pKa = 11.84LPGAMKK223 pKa = 10.19SGSVNTIVSNSIMQDD238 pKa = 2.88LMHH241 pKa = 7.19CYY243 pKa = 9.64ICNKK247 pKa = 9.84LDD249 pKa = 3.47IPVGHH254 pKa = 7.35IWTLGDD260 pKa = 3.73DD261 pKa = 5.05TIQEE265 pKa = 4.48DD266 pKa = 3.48PGEE269 pKa = 4.19EE270 pKa = 4.05YY271 pKa = 10.79FEE273 pKa = 4.16EE274 pKa = 4.28LKK276 pKa = 10.65KK277 pKa = 11.07LCVIKK282 pKa = 10.52EE283 pKa = 4.05IVRR286 pKa = 11.84GTEE289 pKa = 4.01FAGHH293 pKa = 6.35TFDD296 pKa = 5.61GMHH299 pKa = 6.55VSPNYY304 pKa = 9.58VGKK307 pKa = 9.4HH308 pKa = 5.69AYY310 pKa = 8.5TLLHH314 pKa = 6.03VDD316 pKa = 3.71LRR318 pKa = 11.84HH319 pKa = 6.05AKK321 pKa = 8.79EE322 pKa = 3.54ICMSYY327 pKa = 11.05ALLYY331 pKa = 10.62HH332 pKa = 6.76RR333 pKa = 11.84AKK335 pKa = 10.71PSDD338 pKa = 3.39KK339 pKa = 10.62EE340 pKa = 4.28LIYY343 pKa = 10.19TALRR347 pKa = 11.84SLGEE351 pKa = 4.18TLPSEE356 pKa = 4.47AKK358 pKa = 10.31LDD360 pKa = 3.83LVFDD364 pKa = 4.38GSEE367 pKa = 3.61

Molecular weight: 42.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEM3|A0A1L3KEM3_9VIRU C2H2-type domain-containing protein OS=Hubei sobemo-like virus 32 OX=1923219 PE=4 SV=1
MM1 pKa = 7.65EE2 pKa = 6.03FLRR5 pKa = 11.84QLTWFKK11 pKa = 10.24MAWLIAKK18 pKa = 9.55FYY20 pKa = 9.31MGFVMIMNISTILTFLVTLLVSLTWKK46 pKa = 9.39MIKK49 pKa = 9.94KK50 pKa = 9.57VALVDD55 pKa = 3.77LFYY58 pKa = 11.04EE59 pKa = 4.76GFGSVVDD66 pKa = 3.98GEE68 pKa = 4.78VIHH71 pKa = 6.03TVVEE75 pKa = 4.51AVRR78 pKa = 11.84EE79 pKa = 4.1AHH81 pKa = 6.19EE82 pKa = 4.07ATRR85 pKa = 11.84PVEE88 pKa = 4.1PPSWWEE94 pKa = 3.52EE95 pKa = 4.05AYY97 pKa = 10.66VWLMDD102 pKa = 3.52MAMDD106 pKa = 3.92NFDD109 pKa = 3.72VLRR112 pKa = 11.84ICLVMVAGLLVLLAVKK128 pKa = 10.33RR129 pKa = 11.84LVWRR133 pKa = 11.84RR134 pKa = 11.84AKK136 pKa = 10.53RR137 pKa = 11.84LVYY140 pKa = 9.48TMKK143 pKa = 10.65GFHH146 pKa = 6.86CEE148 pKa = 3.73AMVEE152 pKa = 4.29GSEE155 pKa = 4.45FGSGDD160 pKa = 3.16IPSFQIPMYY169 pKa = 9.8RR170 pKa = 11.84AGIFVSTFLGYY181 pKa = 9.87GVRR184 pKa = 11.84VDD186 pKa = 3.58NTLCVPTHH194 pKa = 5.4VVNLARR200 pKa = 11.84GGQLMADD207 pKa = 3.52TWILPRR213 pKa = 11.84TGILSKK219 pKa = 10.34LHH221 pKa = 6.95PDD223 pKa = 2.83VTYY226 pKa = 10.84YY227 pKa = 10.62YY228 pKa = 10.41LPEE231 pKa = 4.3KK232 pKa = 8.69TWSVLATSKK241 pKa = 10.78PKK243 pKa = 10.47VGPVMAAVVVSCAGPDD259 pKa = 4.01GVSHH263 pKa = 5.93GHH265 pKa = 6.78LRR267 pKa = 11.84QTPDD271 pKa = 3.38LGQVTYY277 pKa = 10.71SGSTLPGFSGAAYY290 pKa = 10.41FNGDD294 pKa = 3.89VLFGMHH300 pKa = 6.49TGVACEE306 pKa = 3.99RR307 pKa = 11.84NIGVAAHH314 pKa = 7.15LLQFEE319 pKa = 4.16ARR321 pKa = 11.84EE322 pKa = 4.1AFGQMEE328 pKa = 4.75YY329 pKa = 10.59KK330 pKa = 10.76GEE332 pKa = 4.15SPLNDD337 pKa = 3.32KK338 pKa = 8.61THH340 pKa = 7.12DD341 pKa = 3.9KK342 pKa = 10.83VLKK345 pKa = 7.08TQNRR349 pKa = 11.84VVTWKK354 pKa = 10.82SSDD357 pKa = 3.22ILEE360 pKa = 4.46KK361 pKa = 10.71AKK363 pKa = 10.76KK364 pKa = 9.4LWGPAGSSWADD375 pKa = 3.19EE376 pKa = 4.36VEE378 pKa = 4.43RR379 pKa = 11.84EE380 pKa = 3.76FGEE383 pKa = 4.38YY384 pKa = 10.08EE385 pKa = 4.03NAADD389 pKa = 4.63PLDD392 pKa = 4.65EE393 pKa = 5.81IISEE397 pKa = 4.56LDD399 pKa = 3.29DD400 pKa = 4.02ASKK403 pKa = 10.99RR404 pKa = 11.84KK405 pKa = 9.51ILAKK409 pKa = 10.6LMNSTEE415 pKa = 4.18KK416 pKa = 10.69VVVMGQAPVTATMEE430 pKa = 4.18WEE432 pKa = 4.64KK433 pKa = 10.73EE434 pKa = 4.11NPMHH438 pKa = 7.41VLMEE442 pKa = 4.28NLVATVNEE450 pKa = 3.88QEE452 pKa = 4.41TIIRR456 pKa = 11.84RR457 pKa = 11.84HH458 pKa = 4.62GAEE461 pKa = 3.89INGLKK466 pKa = 10.63AKK468 pKa = 9.8VANLEE473 pKa = 4.08KK474 pKa = 10.76KK475 pKa = 8.96RR476 pKa = 11.84TFAVNEE482 pKa = 4.11SGLRR486 pKa = 11.84DD487 pKa = 3.87CLKK490 pKa = 10.73SAGDD494 pKa = 3.9KK495 pKa = 10.63LVSAANSIGNAIVKK509 pKa = 9.57VAEE512 pKa = 4.13EE513 pKa = 3.99VSPVRR518 pKa = 11.84EE519 pKa = 4.2GEE521 pKa = 3.74KK522 pKa = 10.92DD523 pKa = 3.49EE524 pKa = 6.39IEE526 pKa = 4.12MQEE529 pKa = 3.76ITDD532 pKa = 3.84SVEE535 pKa = 3.74TAEE538 pKa = 4.28EE539 pKa = 4.05VGKK542 pKa = 10.1NLEE545 pKa = 4.52PDD547 pKa = 3.91DD548 pKa = 4.01SQKK551 pKa = 10.96VYY553 pKa = 11.27NPTTMKK559 pKa = 10.08IKK561 pKa = 10.39CFHH564 pKa = 6.84CEE566 pKa = 3.33RR567 pKa = 11.84TFRR570 pKa = 11.84TMLGLKK576 pKa = 4.86THH578 pKa = 6.37KK579 pKa = 9.47MVKK582 pKa = 9.84HH583 pKa = 5.89EE584 pKa = 4.59SAFKK588 pKa = 10.62SDD590 pKa = 3.19FRR592 pKa = 11.84NPVKK596 pKa = 10.3MQGNAFLGKK605 pKa = 10.06RR606 pKa = 11.84YY607 pKa = 9.26PSRR610 pKa = 11.84NLSRR614 pKa = 11.84TSRR617 pKa = 11.84SSNDD621 pKa = 3.08SNQSMSRR628 pKa = 11.84SANPRR633 pKa = 11.84GAPYY637 pKa = 10.66TMEE640 pKa = 3.91QLKK643 pKa = 10.46KK644 pKa = 10.54VLSEE648 pKa = 4.46LLPTMVGQSSEE659 pKa = 4.08KK660 pKa = 9.4QQNN663 pKa = 3.0

Molecular weight: 74.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1030

367

663

515.0

58.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.408 ± 1.048

1.553 ± 0.321

5.146 ± 0.853

7.184 ± 0.609

3.689 ± 0.075

6.311 ± 0.441

2.913 ± 0.601

4.466 ± 0.643

6.408 ± 0.49

8.932 ± 0.17

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

4.369 ± 0.144

3.495 ± 0.534

4.272 ± 0.184

2.913 ± 0.183

5.146 ± 0.295

6.505 ± 0.261

5.534 ± 0.322

9.417 ± 0.218

2.427 ± 0.431

2.913 ± 0.462
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski