Acidilobus sp. SCGC AC-742_M05

Taxonomy: cellular organisms; Archaea; TACK group; Crenarchaeota; Thermoprotei; Acidilobales; Acidilobaceae; Acidilobus; unclassified Acidilobus

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 281 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T9WKN9|A0A2T9WKN9_9CREN Uncharacterized protein (Fragment) OS=Acidilobus sp. SCGC AC-742_M05 OX=1987489 GN=DDW02_01185 PE=4 SV=1
AA1 pKa = 7.34EE2 pKa = 4.61LVWGGGGNGAPTQFVHH18 pKa = 6.86LNSKK22 pKa = 10.4LGLYY26 pKa = 8.92YY27 pKa = 10.96VNSSGKK33 pKa = 8.02LTPMPSLYY41 pKa = 10.59TFGSDD46 pKa = 3.28TAEE49 pKa = 3.96SAYY52 pKa = 10.5NVHH55 pKa = 7.35DD56 pKa = 3.96SLVNGVPNADD66 pKa = 5.0AGSEE70 pKa = 4.0WLGVLTNDD78 pKa = 3.37FNVYY82 pKa = 10.27LISGG86 pKa = 4.05

Molecular weight: 9.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T9WKG5|A0A2T9WKG5_9CREN 50S ribosomal protein L31e OS=Acidilobus sp. SCGC AC-742_M05 OX=1987489 GN=rpl31e PE=3 SV=1
MM1 pKa = 7.42SRR3 pKa = 11.84HH4 pKa = 5.71KK5 pKa = 10.51PLARR9 pKa = 11.84KK10 pKa = 9.13LRR12 pKa = 11.84LARR15 pKa = 11.84ASKK18 pKa = 10.49SSQPVPAWVIVKK30 pKa = 7.32TLRR33 pKa = 11.84KK34 pKa = 8.6FTFNPKK40 pKa = 8.36RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.36WRR45 pKa = 11.84STKK48 pKa = 10.73LKK50 pKa = 9.86VV51 pKa = 3.04

Molecular weight: 6.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

281

0

281

69221

14

1181

246.3

27.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.207 ± 0.148

0.719 ± 0.062

4.844 ± 0.098

7.597 ± 0.166

2.951 ± 0.082

7.768 ± 0.112

1.404 ± 0.053

5.451 ± 0.101

5.035 ± 0.123

11.091 ± 0.181

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.223 ± 0.062

2.392 ± 0.079

4.905 ± 0.101

2.232 ± 0.079

7.518 ± 0.152

6.39 ± 0.138

4.333 ± 0.091

9.441 ± 0.132

1.159 ± 0.066

3.341 ± 0.096
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski