Sutterella wadsworthensis 2_1_59BFAA

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Sutterellaceae; Sutterella; Sutterella wadsworthensis

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2327 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|K1KID3|K1KID3_9BURK Uncharacterized protein OS=Sutterella wadsworthensis 2_1_59BFAA OX=742823 GN=HMPREF9465_00864 PE=4 SV=1
MM1 pKa = 7.54TISEE5 pKa = 5.04LIEE8 pKa = 3.83LLQEE12 pKa = 3.96IMEE15 pKa = 4.38KK16 pKa = 10.7EE17 pKa = 3.72GDD19 pKa = 3.41IEE21 pKa = 4.61VAYY24 pKa = 9.17TYY26 pKa = 11.39NDD28 pKa = 2.73GGYY31 pKa = 9.69PMMGEE36 pKa = 4.59EE37 pKa = 4.17YY38 pKa = 10.64AGGVEE43 pKa = 4.94VRR45 pKa = 11.84LTPYY49 pKa = 10.56GKK51 pKa = 10.27AVVIWW56 pKa = 3.62

Molecular weight:
6.3 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|K1JFF1|K1JFF1_9BURK Uncharacterized protein (Fragment) OS=Sutterella wadsworthensis 2_1_59BFAA OX=742823 GN=HMPREF9465_02131 PE=4 SV=1
MM1 pKa = 8.02RR2 pKa = 11.84DD3 pKa = 3.18TRR5 pKa = 11.84KK6 pKa = 9.85IEE8 pKa = 4.05ADD10 pKa = 3.48VNNPRR15 pKa = 11.84LRR17 pKa = 11.84RR18 pKa = 11.84MFPRR22 pKa = 11.84GNSMAALLKK31 pKa = 10.87LLLFTLLMHH40 pKa = 6.46TACTSLRR47 pKa = 11.84FTATLDD53 pKa = 4.25AIHH56 pKa = 7.0AGASSFEE63 pKa = 4.19VGLMLASISFGPMFLAIAAGRR84 pKa = 11.84WLDD87 pKa = 3.46RR88 pKa = 11.84SGPRR92 pKa = 11.84APMLASRR99 pKa = 11.84LSVMAAAGCAILLPASDD116 pKa = 5.16LGIAPLFGACVLTGLSFMLTNVVIQRR142 pKa = 11.84LTGDD146 pKa = 3.08VTTPKK151 pKa = 10.15NRR153 pKa = 11.84RR154 pKa = 11.84FAFTALSLTTASSNLAAPVVAGYY177 pKa = 10.38VIEE180 pKa = 4.33RR181 pKa = 11.84LSYY184 pKa = 7.78TAVYY188 pKa = 8.96VWSAVAPLILSVLLFVPAFGIVMKK212 pKa = 10.58ARR214 pKa = 11.84PRR216 pKa = 11.84AVRR219 pKa = 11.84AEE221 pKa = 3.85AGRR224 pKa = 11.84TKK226 pKa = 10.85GSAADD231 pKa = 3.85FLRR234 pKa = 11.84DD235 pKa = 3.3GPMRR239 pKa = 11.84AVLVASVIISIAWEE253 pKa = 4.06VGNLLIPIYY262 pKa = 10.42AAGVGLTPSEE272 pKa = 4.25IGWVLGSFAAATFIVRR288 pKa = 11.84FVTPFLLKK296 pKa = 10.6VVLEE300 pKa = 3.65WHH302 pKa = 6.59MIVLSLLLATAAFAVMPLVTTQGAMMGAAFVLGLGLGASLPNMMSLVYY350 pKa = 10.39LFSPPEE356 pKa = 4.38RR357 pKa = 11.84IGEE360 pKa = 4.38AIGLRR365 pKa = 11.84IMALNLGKK373 pKa = 10.37SVFPVLAGLLGNWIGAGSTIWCLAAFAASGCGYY406 pKa = 10.53AGAAARR412 pKa = 11.84TVLSRR417 pKa = 11.84MKK419 pKa = 10.51ALRR422 pKa = 11.84DD423 pKa = 3.66AKK425 pKa = 10.56IDD427 pKa = 3.6GG428 pKa = 3.95

Molecular weight:
45.08 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2327

0

2327

737528

29

1908

316.9

34.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.211 ± 0.069

1.32 ± 0.022

5.463 ± 0.039

6.541 ± 0.052

3.86 ± 0.041

7.976 ± 0.053

1.932 ± 0.022

5.003 ± 0.035

4.435 ± 0.053

9.661 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.779 ± 0.022

2.99 ± 0.036

4.581 ± 0.037

2.795 ± 0.026

6.848 ± 0.072

5.584 ± 0.039

5.595 ± 0.038

7.787 ± 0.045

1.3 ± 0.02

2.339 ± 0.029

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski