Xanthomonas phage f20-Xaj

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Pradovirus; Xanthomonas virus f20

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A127AVI6|A0A127AVI6_9CAUD Uncharacterized protein OS=Xanthomonas phage f20-Xaj OX=1784979 PE=4 SV=1
MM1 pKa = 7.6VNITVEE7 pKa = 4.24EE8 pKa = 4.07YY9 pKa = 10.58DD10 pKa = 3.74ALVAAKK16 pKa = 10.55DD17 pKa = 3.77EE18 pKa = 4.47LDD20 pKa = 3.68ALHH23 pKa = 7.0AGGVSSWDD31 pKa = 3.24WYY33 pKa = 10.57DD34 pKa = 4.56QSLQDD39 pKa = 5.3AGFFDD44 pKa = 4.8QEE46 pKa = 4.78DD47 pKa = 4.19EE48 pKa = 4.63EE49 pKa = 4.72EE50 pKa = 4.27DD51 pKa = 3.43

Molecular weight: 5.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A127AVH8|A0A127AVH8_9CAUD Uncharacterized protein OS=Xanthomonas phage f20-Xaj OX=1784979 PE=4 SV=1
MM1 pKa = 7.39KK2 pKa = 10.41RR3 pKa = 11.84LTATQVKK10 pKa = 9.51LVRR13 pKa = 11.84VKK15 pKa = 10.34IAAEE19 pKa = 3.76QGNRR23 pKa = 11.84CALCGGQFGIKK34 pKa = 10.23APLDD38 pKa = 3.57PCLDD42 pKa = 3.58HH43 pKa = 7.33DD44 pKa = 4.46HH45 pKa = 6.57RR46 pKa = 11.84TGAVRR51 pKa = 11.84GVLHH55 pKa = 6.87RR56 pKa = 11.84GCNALLGRR64 pKa = 11.84VEE66 pKa = 4.49NNAPRR71 pKa = 11.84YY72 pKa = 8.79GVRR75 pKa = 11.84DD76 pKa = 3.33VFAFSSGIATYY87 pKa = 10.54LRR89 pKa = 11.84QHH91 pKa = 5.54MTNITGYY98 pKa = 10.24LHH100 pKa = 6.52PTHH103 pKa = 6.24KK104 pKa = 9.81TEE106 pKa = 4.23DD107 pKa = 3.4EE108 pKa = 3.84KK109 pKa = 11.41RR110 pKa = 11.84IARR113 pKa = 11.84NTKK116 pKa = 9.27ARR118 pKa = 11.84KK119 pKa = 7.3TRR121 pKa = 11.84LKK123 pKa = 10.68KK124 pKa = 10.58KK125 pKa = 8.19EE126 pKa = 4.05TLL128 pKa = 3.51

Molecular weight: 14.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

13382

41

1636

252.5

27.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.507 ± 0.673

0.822 ± 0.15

6.187 ± 0.152

5.911 ± 0.255

3.303 ± 0.164

8.287 ± 0.315

1.958 ± 0.204

4.588 ± 0.177

4.954 ± 0.384

8.003 ± 0.182

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.757 ± 0.2

4.274 ± 0.214

4.088 ± 0.26

4.514 ± 0.248

6.113 ± 0.332

5.806 ± 0.274

6.24 ± 0.285

6.688 ± 0.297

1.644 ± 0.109

3.355 ± 0.18
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski