Nocardia donostiensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Nocardiaceae; Nocardia

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4892 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W0ARL6|A0A1W0ARL6_9NOCA Mammalian cell entry protein OS=Nocardia donostiensis OX=1538463 GN=B0T46_07050 PE=4 SV=1
MM1 pKa = 7.8TDD3 pKa = 3.14YY4 pKa = 11.52KK5 pKa = 10.8LYY7 pKa = 11.0QCMQCGFEE15 pKa = 3.99YY16 pKa = 10.83DD17 pKa = 3.73EE18 pKa = 4.64AKK20 pKa = 10.39GWPEE24 pKa = 4.22DD25 pKa = 4.75DD26 pKa = 3.61IAPGTRR32 pKa = 11.84WDD34 pKa = 5.72DD35 pKa = 3.49IPEE38 pKa = 4.29DD39 pKa = 3.99WTCPDD44 pKa = 3.98CGAAKK49 pKa = 10.47ADD51 pKa = 3.6FFMIEE56 pKa = 4.06IEE58 pKa = 4.19RR59 pKa = 11.84SS60 pKa = 3.26

Molecular weight: 7.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W0AWI3|A0A1W0AWI3_9NOCA Uncharacterized protein OS=Nocardia donostiensis OX=1538463 GN=B0T46_25520 PE=4 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVSSRR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 9.76GRR42 pKa = 11.84ASLTAA47 pKa = 4.1

Molecular weight: 5.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4892

0

4892

1613563

31

23708

329.8

35.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.22 ± 0.052

0.744 ± 0.014

6.23 ± 0.032

5.673 ± 0.043

2.869 ± 0.022

8.856 ± 0.038

2.27 ± 0.017

4.113 ± 0.026

1.875 ± 0.03

10.036 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.87 ± 0.019

1.976 ± 0.017

5.986 ± 0.03

3.023 ± 0.024

7.767 ± 0.041

5.198 ± 0.024

6.227 ± 0.028

8.535 ± 0.038

1.44 ± 0.013

2.094 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski