endosymbiont TC1 of Trimyema compressum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; unclassified Firmicutes sensu stricto

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1674 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A191HTH6|A0A191HTH6_9FIRM Uncharacterized protein OS=endosymbiont 'TC1' of Trimyema compressum OX=243899 GN=AZF37_02700 PE=4 SV=1
MM1 pKa = 6.86FTSFDD6 pKa = 4.64FIQTYY11 pKa = 7.58SLKK14 pKa = 10.75AKK16 pKa = 10.04EE17 pKa = 4.46SLVDD21 pKa = 3.59AQGVGNATIPTGVKK35 pKa = 9.72FATEE39 pKa = 4.09GQVTFAQQFPNAHH52 pKa = 6.05TADD55 pKa = 3.28VMASYY60 pKa = 10.36FGKK63 pKa = 10.31SVSDD67 pKa = 4.24VIDD70 pKa = 3.57QNILEE75 pKa = 4.29TEE77 pKa = 4.08YY78 pKa = 11.16LYY80 pKa = 11.33LSDD83 pKa = 5.66NMLIDD88 pKa = 3.18ITGIDD93 pKa = 3.98IFTNLQKK100 pKa = 10.97LYY102 pKa = 10.77LDD104 pKa = 4.23SNQLASIPNSIDD116 pKa = 3.08NLSNVQTLSLGYY128 pKa = 9.75NQLTSIPQSIGFF140 pKa = 3.98

Molecular weight: 15.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A191HWY7|A0A191HWY7_9FIRM Uncharacterized protein OS=endosymbiont 'TC1' of Trimyema compressum OX=243899 GN=AZF37_09045 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84KK11 pKa = 8.21QKK13 pKa = 8.12RR14 pKa = 11.84TSGFLVRR21 pKa = 11.84MRR23 pKa = 11.84SSNGKK28 pKa = 9.04NVIKK32 pKa = 10.5RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.26GKK39 pKa = 10.34KK40 pKa = 9.15KK41 pKa = 10.78LSVV44 pKa = 3.13

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1674

0

1674

368452

37

1423

220.1

24.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.211 ± 0.064

1.059 ± 0.022

5.146 ± 0.047

7.374 ± 0.08

4.485 ± 0.059

7.193 ± 0.059

1.591 ± 0.025

8.931 ± 0.069

8.239 ± 0.064

9.929 ± 0.076

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.66 ± 0.028

5.258 ± 0.055

3.264 ± 0.033

2.695 ± 0.028

3.599 ± 0.046

5.938 ± 0.054

5.233 ± 0.046

6.76 ± 0.052

0.676 ± 0.022

3.76 ± 0.044
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski