Garlic virus E

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Alphaflexiviridae; Allexivirus; Acarallexivirus

Average proteome isoelectric point is 6.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|Q8QXW5|Q8QXW5_9VIRU RNA-directed RNA polymerase OS=Garlic virus E OX=150285 PE=4 SV=1
MM1 pKa = 6.78TTVQKK6 pKa = 11.0LLDD9 pKa = 4.13QISDD13 pKa = 3.75PSARR17 pKa = 11.84AAYY20 pKa = 10.18SQDD23 pKa = 3.42CLSAAKK29 pKa = 8.78TAATKK34 pKa = 11.09AMTIAPYY41 pKa = 10.78AVTPTEE47 pKa = 3.96ALTLEE52 pKa = 4.78RR53 pKa = 11.84IGITTSPFATTSHH66 pKa = 4.89THH68 pKa = 5.93AADD71 pKa = 4.1KK72 pKa = 10.63IIEE75 pKa = 4.28NDD77 pKa = 3.51CLDD80 pKa = 3.67IIGRR84 pKa = 11.84YY85 pKa = 8.46LPKK88 pKa = 10.68EE89 pKa = 4.03PVTLIQLKK97 pKa = 9.75RR98 pKa = 11.84AKK100 pKa = 10.22LHH102 pKa = 5.63LLRR105 pKa = 11.84RR106 pKa = 11.84GPSGDD111 pKa = 2.8IFKK114 pKa = 10.65NYY116 pKa = 9.13CHH118 pKa = 6.56EE119 pKa = 4.62PKK121 pKa = 10.48DD122 pKa = 3.63VLRR125 pKa = 11.84FGLTNPHH132 pKa = 6.48TCPQVDD138 pKa = 3.81TACAVISDD146 pKa = 4.26TLHH149 pKa = 6.45FMSTHH154 pKa = 4.83QVWTLFQNNPKK165 pKa = 10.35LEE167 pKa = 4.08RR168 pKa = 11.84LYY170 pKa = 10.23GTLVLPVEE178 pKa = 4.86ALHH181 pKa = 6.49KK182 pKa = 10.57LPSLFPEE189 pKa = 4.6IYY191 pKa = 9.95KK192 pKa = 10.38LEE194 pKa = 4.14YY195 pKa = 9.81YY196 pKa = 9.52EE197 pKa = 4.72RR198 pKa = 11.84HH199 pKa = 5.31FAYY202 pKa = 9.93MPGGYY207 pKa = 10.15GGGAYY212 pKa = 8.77IHH214 pKa = 7.09SYY216 pKa = 8.85GTLKK220 pKa = 10.24WLSTAQIGPGAYY232 pKa = 9.03PGYY235 pKa = 9.94RR236 pKa = 11.84PPANGRR242 pKa = 11.84RR243 pKa = 11.84PLLDD247 pKa = 3.43HH248 pKa = 7.0LSIEE252 pKa = 4.43KK253 pKa = 10.26IEE255 pKa = 4.33TKK257 pKa = 10.09AAHH260 pKa = 6.81HH261 pKa = 6.24IVMIQRR267 pKa = 11.84RR268 pKa = 11.84RR269 pKa = 11.84PEE271 pKa = 4.54LPWPLPPIWVFHH283 pKa = 6.41ASDD286 pKa = 4.04YY287 pKa = 11.35VKK289 pKa = 10.55LPEE292 pKa = 3.88IFYY295 pKa = 9.44PAEE298 pKa = 4.09ANVQKK303 pKa = 10.61AYY305 pKa = 9.7PHH307 pKa = 5.81TFIKK311 pKa = 10.5RR312 pKa = 11.84LQLYY316 pKa = 8.31CFSVKK321 pKa = 10.2AVSLRR326 pKa = 11.84DD327 pKa = 3.18IFAKK331 pKa = 9.72IRR333 pKa = 11.84QVIPTEE339 pKa = 3.68EE340 pKa = 4.04LARR343 pKa = 11.84FSTADD348 pKa = 4.67LIRR351 pKa = 11.84LANYY355 pKa = 10.44LLFITGMQSASDD367 pKa = 3.9YY368 pKa = 10.64QSPLMEE374 pKa = 4.71SLLGKK379 pKa = 10.15LCLSIRR385 pKa = 11.84ARR387 pKa = 11.84VRR389 pKa = 11.84EE390 pKa = 4.06FFQNILGKK398 pKa = 10.11SSYY401 pKa = 10.85AALLTVTDD409 pKa = 3.44VRR411 pKa = 11.84PIHH414 pKa = 6.0FTTKK418 pKa = 7.78PQRR421 pKa = 11.84RR422 pKa = 11.84GICGEE427 pKa = 3.78QWFDD431 pKa = 3.84NPEE434 pKa = 3.8EE435 pKa = 4.9DD436 pKa = 5.0EE437 pKa = 5.1AGPPNNSLTGYY448 pKa = 6.96PQPSPDD454 pKa = 4.98DD455 pKa = 4.17LDD457 pKa = 4.22LLAEE461 pKa = 4.37FEE463 pKa = 4.31GHH465 pKa = 6.71AMNPKK470 pKa = 8.83TLSNNTRR477 pKa = 11.84GRR479 pKa = 11.84NDD481 pKa = 3.31FPEE484 pKa = 4.99CPVADD489 pKa = 3.91TTAMNEE495 pKa = 3.56EE496 pKa = 4.31DD497 pKa = 3.61TRR499 pKa = 11.84RR500 pKa = 11.84SHH502 pKa = 7.17YY503 pKa = 10.21EE504 pKa = 3.47EE505 pKa = 4.7HH506 pKa = 6.94AKK508 pKa = 10.03QATTTPNTEE517 pKa = 3.98APKK520 pKa = 10.43EE521 pKa = 3.95ALTSDD526 pKa = 3.22QEE528 pKa = 5.06GYY530 pKa = 8.1VTDD533 pKa = 3.77WSDD536 pKa = 3.91DD537 pKa = 3.68EE538 pKa = 5.17SSSSVKK544 pKa = 10.34PPEE547 pKa = 4.34SEE549 pKa = 4.11GAPLEE554 pKa = 4.88DD555 pKa = 5.88DD556 pKa = 5.34DD557 pKa = 6.9LFDD560 pKa = 5.66LPPLEE565 pKa = 5.53DD566 pKa = 3.59CADD569 pKa = 3.77PVEE572 pKa = 5.61ADD574 pKa = 3.55AQEE577 pKa = 4.74HH578 pKa = 6.39PDD580 pKa = 3.8EE581 pKa = 4.88VSVDD585 pKa = 3.97LPSPDD590 pKa = 3.61SDD592 pKa = 3.59HH593 pKa = 6.72AHH595 pKa = 5.56TGEE598 pKa = 4.22RR599 pKa = 11.84TLEE602 pKa = 4.07TPEE605 pKa = 4.89DD606 pKa = 4.22SVTGEE611 pKa = 4.12AQNCDD616 pKa = 3.13GSRR619 pKa = 11.84HH620 pKa = 5.18IDD622 pKa = 3.44EE623 pKa = 4.89PEE625 pKa = 3.74EE626 pKa = 3.92RR627 pKa = 11.84GQPSKK632 pKa = 9.41QTCKK636 pKa = 10.82VEE638 pKa = 4.0LTPLDD643 pKa = 4.09ADD645 pKa = 3.84TVEE648 pKa = 4.06LLKK651 pKa = 11.2LHH653 pKa = 6.74GFTNYY658 pKa = 9.23RR659 pKa = 11.84AQYY662 pKa = 9.98DD663 pKa = 4.29GEE665 pKa = 4.37SQIQPIYY672 pKa = 10.1RR673 pKa = 11.84NPHH676 pKa = 4.15AHH678 pKa = 7.48FLRR681 pKa = 11.84QYY683 pKa = 10.24DD684 pKa = 3.83AQTTTHH690 pKa = 5.3QQQFVDD696 pKa = 3.71MAHH699 pKa = 5.79GLKK702 pKa = 10.05RR703 pKa = 11.84RR704 pKa = 11.84CYY706 pKa = 10.04GYY708 pKa = 10.91KK709 pKa = 9.94VDD711 pKa = 4.93AKK713 pKa = 10.44RR714 pKa = 11.84ATAFMSDD721 pKa = 3.27VKK723 pKa = 11.38NNLTGLVLPKK733 pKa = 10.1LDD735 pKa = 4.65RR736 pKa = 11.84DD737 pKa = 4.19LLSSWVALAEE747 pKa = 4.14NATRR751 pKa = 11.84TVGLIVIHH759 pKa = 6.17GAGGAGKK766 pKa = 10.07SRR768 pKa = 11.84ALQEE772 pKa = 3.92LLRR775 pKa = 11.84NRR777 pKa = 11.84VVDD780 pKa = 4.01ANEE783 pKa = 3.77VNIVVPTINLAADD796 pKa = 3.71WKK798 pKa = 10.58KK799 pKa = 10.77KK800 pKa = 10.55LPALDD805 pKa = 3.76PRR807 pKa = 11.84RR808 pKa = 11.84VMTFEE813 pKa = 4.09KK814 pKa = 10.45ACEE817 pKa = 3.99RR818 pKa = 11.84EE819 pKa = 4.42GKK821 pKa = 10.11SVVIMDD827 pKa = 5.34DD828 pKa = 3.58YY829 pKa = 11.94GKK831 pKa = 10.9LPAGYY836 pKa = 9.92VDD838 pKa = 5.04AYY840 pKa = 11.11LAIKK844 pKa = 10.64SNVEE848 pKa = 4.21LIVLTGDD855 pKa = 3.3QRR857 pKa = 11.84QSVYY861 pKa = 11.02HH862 pKa = 5.98NDD864 pKa = 3.26KK865 pKa = 10.49RR866 pKa = 11.84DD867 pKa = 3.5SQIALLSPNTEE878 pKa = 3.8HH879 pKa = 7.44FKK881 pKa = 10.58RR882 pKa = 11.84YY883 pKa = 9.09CDD885 pKa = 3.63YY886 pKa = 11.06YY887 pKa = 11.31VNATHH892 pKa = 6.72RR893 pKa = 11.84QPRR896 pKa = 11.84RR897 pKa = 11.84LANPIRR903 pKa = 11.84VHH905 pKa = 6.64AEE907 pKa = 3.84RR908 pKa = 11.84EE909 pKa = 3.82VGGAVKK915 pKa = 10.02HH916 pKa = 5.55ATLIPTNAMTLVPAFRR932 pKa = 11.84SQSLLTDD939 pKa = 4.09LGRR942 pKa = 11.84QAMTYY947 pKa = 10.18AGCQGLTLPHH957 pKa = 6.01LTIVLDD963 pKa = 4.36KK964 pKa = 10.04DD965 pKa = 3.68TPLCSDD971 pKa = 3.5EE972 pKa = 4.38VLYY975 pKa = 9.62TALSRR980 pKa = 11.84ASEE983 pKa = 4.34TITFVNTHH991 pKa = 5.86SDD993 pKa = 3.18NKK995 pKa = 10.56EE996 pKa = 3.68FLAKK1000 pKa = 10.27LDD1002 pKa = 3.46ATPYY1006 pKa = 10.68LKK1008 pKa = 9.78TLISGVRR1015 pKa = 11.84EE1016 pKa = 4.11DD1017 pKa = 3.91EE1018 pKa = 4.22QSGGDD1023 pKa = 3.79YY1024 pKa = 10.45QPPEE1028 pKa = 3.88PQVRR1032 pKa = 11.84DD1033 pKa = 3.95GPTKK1037 pKa = 9.4THH1039 pKa = 6.14MPVANNNIQLEE1050 pKa = 4.44EE1051 pKa = 4.88KK1052 pKa = 10.35IEE1054 pKa = 4.04TMEE1057 pKa = 5.18DD1058 pKa = 3.12KK1059 pKa = 10.11DD1060 pKa = 4.63TRR1062 pKa = 11.84EE1063 pKa = 3.87LWSGEE1068 pKa = 3.84EE1069 pKa = 4.18KK1070 pKa = 10.4TNLMQTQDD1078 pKa = 3.55PVVQLFPHH1086 pKa = 5.42QQSKK1090 pKa = 11.5DD1091 pKa = 3.3EE1092 pKa = 4.42ALFKK1096 pKa = 10.16ITIKK1100 pKa = 10.57EE1101 pKa = 4.23RR1102 pKa = 11.84IRR1104 pKa = 11.84TADD1107 pKa = 2.85VGTNKK1112 pKa = 10.11KK1113 pKa = 9.88ALCEE1117 pKa = 3.9TLSAGDD1123 pKa = 4.57LLFEE1127 pKa = 5.24AYY1129 pKa = 10.06AKK1131 pKa = 10.37FMNVPQEE1138 pKa = 4.0TQPFDD1143 pKa = 3.35KK1144 pKa = 10.7RR1145 pKa = 11.84LWAHH1149 pKa = 6.7CKK1151 pKa = 9.57QLALRR1156 pKa = 11.84TYY1158 pKa = 10.57LSKK1161 pKa = 9.75PTTNLQQGAQRR1172 pKa = 11.84QDD1174 pKa = 2.93PDD1176 pKa = 3.87FPDD1179 pKa = 3.11NAIALFNKK1187 pKa = 8.34SQWVKK1192 pKa = 10.35KK1193 pKa = 10.25LEE1195 pKa = 4.06KK1196 pKa = 10.47VGCKK1200 pKa = 9.95FKK1202 pKa = 11.02AGQTISAFKK1211 pKa = 10.55QSTVLLTTTMALYY1224 pKa = 10.13LRR1226 pKa = 11.84KK1227 pKa = 9.51KK1228 pKa = 10.63RR1229 pKa = 11.84EE1230 pKa = 3.53GHH1232 pKa = 5.43QPDD1235 pKa = 3.93NVFVMCEE1242 pKa = 3.82KK1243 pKa = 10.29TPEE1246 pKa = 4.06QFDD1249 pKa = 4.07SFVLTKK1255 pKa = 9.99WDD1257 pKa = 4.4FSRR1260 pKa = 11.84PNYY1263 pKa = 9.07TSDD1266 pKa = 3.01YY1267 pKa = 8.03TQYY1270 pKa = 11.28DD1271 pKa = 3.62QSQDD1275 pKa = 3.09AAFLNFEE1282 pKa = 4.32LRR1284 pKa = 11.84KK1285 pKa = 9.67ARR1287 pKa = 11.84HH1288 pKa = 5.43FGIPNEE1294 pKa = 4.21VVDD1297 pKa = 6.08FYY1299 pKa = 12.1AFIKK1303 pKa = 8.86THH1305 pKa = 6.42AKK1307 pKa = 9.19TFLGNLAIMRR1317 pKa = 11.84LSGEE1321 pKa = 4.31GPTFDD1326 pKa = 6.1ANTEE1330 pKa = 4.05CNIAYY1335 pKa = 8.27DD1336 pKa = 3.69ALRR1339 pKa = 11.84FQVDD1343 pKa = 3.65STVNACYY1350 pKa = 10.3AGDD1353 pKa = 3.77DD1354 pKa = 3.85LVRR1357 pKa = 11.84DD1358 pKa = 4.11KK1359 pKa = 11.41ACEE1362 pKa = 3.73EE1363 pKa = 4.09RR1364 pKa = 11.84PGWKK1368 pKa = 9.61YY1369 pKa = 11.18AEE1371 pKa = 4.31PLFSLKK1377 pKa = 10.56AKK1379 pKa = 10.13PLVTNKK1385 pKa = 9.9PDD1387 pKa = 3.19FCGWRR1392 pKa = 11.84LTKK1395 pKa = 10.67FGIVKK1400 pKa = 10.5SPVQLYY1406 pKa = 10.28QSLQLALRR1414 pKa = 11.84LGKK1417 pKa = 10.19VEE1419 pKa = 4.03EE1420 pKa = 4.42VKK1422 pKa = 10.49RR1423 pKa = 11.84SYY1425 pKa = 11.23AIDD1428 pKa = 3.38YY1429 pKa = 9.93LFAYY1433 pKa = 10.43RR1434 pKa = 11.84LGDD1437 pKa = 3.26QLYY1440 pKa = 10.87DD1441 pKa = 3.48VFDD1444 pKa = 4.15EE1445 pKa = 4.8NEE1447 pKa = 4.12MEE1449 pKa = 4.04KK1450 pKa = 10.33HH1451 pKa = 5.01QLVTRR1456 pKa = 11.84TLIKK1460 pKa = 10.4KK1461 pKa = 9.38GMRR1464 pKa = 11.84PPSSGDD1470 pKa = 3.29HH1471 pKa = 6.27LPVFHH1476 pKa = 6.29VTSDD1480 pKa = 3.09RR1481 pKa = 11.84LIRR1484 pKa = 11.84DD1485 pKa = 4.04PKK1487 pKa = 10.22AKK1489 pKa = 10.21HH1490 pKa = 5.61ISTYY1494 pKa = 10.26EE1495 pKa = 3.88YY1496 pKa = 10.7DD1497 pKa = 3.9STSLPFNIVEE1507 pKa = 4.47DD1508 pKa = 3.72HH1509 pKa = 6.64FAYY1512 pKa = 10.62NPNRR1516 pKa = 11.84ADD1518 pKa = 4.09RR1519 pKa = 11.84DD1520 pKa = 3.97DD1521 pKa = 3.77MNMARR1526 pKa = 11.84DD1527 pKa = 4.03SQSHH1531 pKa = 4.56EE1532 pKa = 3.98TSNASNFPPARR1543 pKa = 11.84EE1544 pKa = 4.08PLLSDD1549 pKa = 4.9LFPQLSSLL1557 pKa = 3.93

Molecular weight:
175.71 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|Q8QXV9|Q8QXV9_9VIRU Coat protein OS=Garlic virus E OX=150285 PE=4 SV=1
MM1 pKa = 7.51HH2 pKa = 7.32PHH4 pKa = 7.33DD5 pKa = 5.95FNLLCCLHH13 pKa = 6.73FSPNHH18 pKa = 5.86LPKK21 pKa = 10.63DD22 pKa = 3.61LQILIFSRR30 pKa = 11.84VTTEE34 pKa = 3.62CKK36 pKa = 9.88LRR38 pKa = 11.84RR39 pKa = 11.84LSEE42 pKa = 4.03NKK44 pKa = 9.53PFRR47 pKa = 11.84GRR49 pKa = 11.84SKK51 pKa = 9.6FAMRR55 pKa = 11.84RR56 pKa = 11.84RR57 pKa = 11.84AKK59 pKa = 10.21RR60 pKa = 11.84YY61 pKa = 8.54NRR63 pKa = 11.84CFDD66 pKa = 3.9CGAVLIDD73 pKa = 3.56DD74 pKa = 4.57HH75 pKa = 5.77QCKK78 pKa = 10.21VLTSRR83 pKa = 11.84AQSDD87 pKa = 4.15VLTVIRR93 pKa = 11.84EE94 pKa = 4.5GPAKK98 pKa = 10.35LYY100 pKa = 10.92AEE102 pKa = 3.9RR103 pKa = 11.84TYY105 pKa = 11.12RR106 pKa = 11.84PNSDD110 pKa = 3.17AARR113 pKa = 11.84LIEE116 pKa = 6.11DD117 pKa = 5.06DD118 pKa = 3.12ILYY121 pKa = 10.29IKK123 pKa = 8.26TLKK126 pKa = 10.55LL127 pKa = 3.3

Molecular weight:
14.89 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

3226

103

1557

403.3

45.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.812 ± 0.434

1.984 ± 0.237

6.2 ± 0.452

5.177 ± 0.63

4.123 ± 0.29

5.053 ± 0.515

2.976 ± 0.305

4.061 ± 0.405

4.681 ± 0.676

9.454 ± 0.806

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.108 ± 0.477

4.96 ± 0.654

6.851 ± 0.322

4.805 ± 0.343

6.107 ± 0.411

7.502 ± 0.949

7.316 ± 0.307

5.084 ± 0.155

0.682 ± 0.12

3.069 ± 0.446

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski